ID   RPOB2_CHLRE             Reviewed;         626 AA.
AC   Q8HTL7; B7U1I1;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta C-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta C-terminal section;
DE            Short=RNA polymerase subunit beta C-terminal section;
GN   Name=rpoB2;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1617738; DOI=10.1007/bf00351659;
RA   Fong S.E., Surzycki S.J.;
RT   "Chloroplast RNA polymerase genes of Chlamydomonas reinhardtii exhibit an
RT   unusual structure and arrangement.";
RL   Curr. Genet. 21:485-497(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
RN   [3]
RP   IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In C.reinhardtii the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; AY160684; AAN41267.1; -; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50128.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00942.1; -; Genomic_DNA.
DR   PIR; S26873; S26873.
DR   RefSeq; NP_958397.1; NC_005353.1.
DR   AlphaFoldDB; Q8HTL7; -.
DR   SMR; Q8HTL7; -.
DR   STRING; 3055.DAA00942; -.
DR   PaxDb; Q8HTL7; -.
DR   PRIDE; Q8HTL7; -.
DR   GeneID; 2716992; -.
DR   KEGG; cre:ChreCp041; -.
DR   eggNOG; KOG0214; Eukaryota.
DR   HOGENOM; CLU_000524_4_2_1; -.
DR   InParanoid; Q8HTL7; -.
DR   OrthoDB; 7046at2759; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..626
FT                   /note="DNA-directed RNA polymerase subunit beta C-terminal
FT                   section"
FT                   /id="PRO_0000048017"
FT   REGION          287..307
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   626 AA;  70430 MW;  3E54964DB9C49271 CRC64;
     MTYSILTESI YNFKKKSSLD MQFLSPKNFQ IENFTKIHDQ TLPLKPFKTN RTAVAVVPPL
     PIFSPELYKR QNKLKKTQNA TIFSMGTSAF KNQVKYNLET YHRSNQDTCL IHKPAVKEGD
     WVEVGDLLAD SASSIGGELA IGHNIIVAYM PWEGYNYEDA ILINERLVYE DIYTSIHIER
     YEVTTKETKL GFEQITREIP DISENEIKHL DKTGIAKIGS WVEEGDILVG KITPFNIKTL
     TPQQKLLYKI FDKQLSTTKD SSLRAPKGIK ANVININILA RQKIQINTKS KNTGKGSKPP
     RASKAQNTMV SQPSYIHIYL AEKRKMQVGD KMAGRHGNKG IVSRILPRQD MPFLPDGAAV
     DIVLNPLGVP SRMNVGQIYE CLLGLAGRYL GEHYKIPPFD EMYGADASRS FVLSKLYEAR
     KKTGLKWLLD PNHPGKIRLF DGRNSECFDQ TVTVGIAYVL KLVHMVDDKM HARSTGPYSL
     VTQQPLRGRS KQGGQRLGEM EVWAIEGYGA AFVLSEMLTI KSDDMTGRQN LWKNLIENKE
     ISLGSPESFK VLICELQALC LDIGLFRKNK ETSLPYFKMP GEESKTNVNA NLTGVQSSKK
     LEANSQTVKN SSMPNLVEID NLLNLA