RPOB2_NOCFA
ID RPOB2_NOCFA Reviewed; 1163 AA.
AC Q5YPE0;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=DNA-directed RNA polymerase subunit beta 2 {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta 2 {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta 2 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta 2 {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB2 {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=NFA_50990;
OS Nocardia farcinica (strain IFM 10152).
OC Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX NCBI_TaxID=247156;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IFM 10152;
RX PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA Shiba T., Hattori M.;
RT "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AP006618; BAD59951.1; -; Genomic_DNA.
DR RefSeq; WP_011211633.1; NC_006361.1.
DR AlphaFoldDB; Q5YPE0; -.
DR SMR; Q5YPE0; -.
DR STRING; 247156.NFA_50990; -.
DR PRIDE; Q5YPE0; -.
DR EnsemblBacteria; BAD59951; BAD59951; NFA_50990.
DR GeneID; 61135673; -.
DR KEGG; nfa:NFA_50990; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_11; -.
DR OMA; FMTWEGY; -.
DR BioCyc; NFAR247156:NFA_RS25265-MON; -.
DR Proteomes; UP000006820; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1163
FT /note="DNA-directed RNA polymerase subunit beta 2"
FT /id="PRO_0000224085"
SQ SEQUENCE 1163 AA; 128607 MW; 400DA656CDE80B41 CRC64;
MLEGRILAVS TQTKAVAGIP GAPKRVSFAK IREPLEVPGL LDLQTESFAW LIGSPEWRER
AAARGDVGLV GGLEEVLEEL SPIEDFSGSM SLSFSDPRFE EVKASIDECK EKDMTYAAPL
FVTAEFINNN TGEIKSQTVF MGDFPMMTDK GTFIINGTER VVVSQLVRSP GVYFDHSIDK
GTEKDLHSVR VIPSRGAWLE FDVDKRDTVG VRIDRKRRQP VTVLLKALGW TTEEIAERFG
FSEIMMSTLE KDNTAGQDEA LLDIYRKLRP GEPPTKESAQ TLLENLFFKE KRYDLARVGR
YKINKKLGIH VGEPVTGSVL TKEDIVTTIE YLVRLHAGDK TMTAPGGVEV PVEVDDIDHF
GNRRLRTVGE LIQNQIRVGL SRMERVVRER MTTQDVEAIT PQTLINIRPV VAAIKEFFGT
SQLSQFMDQN NPLSGLTHKR RLSALGPGGL SRERAGLEVR DVHPSHYGRM CPIETPEGPN
IGLIGSLSVY ARVNPFGFIE TPYRKVVDGR VTDEVVYLTA DEEDRHVRAQ ANSPVGPDGR
FLEDRVLCRR GNEEMEYVAA TEVDFMDVSP RQMVSVATAM IPFLEHDDAN RALMGANMQR
QAVPLIRSEA PIVGTGMELR AAVDAGDVVV NEKAGVVEEV SADYVTVMAD DGTRKSYRMR
KFNRSNQGTC SNQRPIVDEG QRVEAGQVLA DGPCTENGEM ALGKNLLVAI MPWEGHNYED
AIILSQRLVE QDVLTSIHIE EHEIDARDTK LGAEEITRDI PNVSDEVLAD LDERGIVRIG
AEVRDGDILV GKVTPKGETE LTPEERLLRA IFGEKAREVR DTSLKVPHGE SGKVIGIRVF
SREDDDDLPP GVNELVRVYV AQKRKIQDGD KLAGRHGNKG VIGKILPTED MPFLPDGTPV
DIILNTHGVP RRMNIGQILE THLGWIGKAG WKVEGNPEWA KDLPEEMWEA PADSNIATPV
FDGAREEELT GLLGSTLPNR DGERMVDDNG KAVLFDGRSG EPFPYPVAVG YMYILKLHHL
VDDKIHARST GPYSMITQQP LGGKAQFGGQ RFGEMECWAM QAYGAAYTLQ ELLTIKSDDV
VGRVKVYEAI VKGENIPEPG IPESFKVLLK ELQSLCLNVE VLSSDGAAIE LREGEDEDLE
RAAANLGINL SRNEAATVDD LAN
