ID   RPOB2_PLETE             Reviewed;         880 AA.
AC   A6YGE0;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta C-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta C-terminal section;
DE            Short=RNA polymerase subunit beta C-terminal section;
GN   Name=rpoB2; Synonyms=rpoBb;
OS   Pleurastrum terricola (Filamentous green alga) (Leptosira terrestris).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Pleurastraceae; Pleurastrum.
OX   NCBI_TaxID=34116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCAP 463/2 / UTEX 333;
RX   PubMed=17610731; DOI=10.1186/1471-2164-8-213;
RA   de Cambiaire J.-C., Otis C., Turmel M., Lemieux C.;
RT   "The chloroplast genome sequence of the green alga Leptosira terrestris:
RT   multiple losses of the inverted repeat and extensive genome rearrangements
RT   within the Trebouxiophyceae.";
RL   BMC Genomics 8:213-213(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In L.terrestris the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; EF506945; ABO69352.1; -; Genomic_DNA.
DR   RefSeq; YP_001382217.1; NC_009681.1.
DR   AlphaFoldDB; A6YGE0; -.
DR   GeneID; 5383754; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 2.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 2.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..880
FT                   /note="DNA-directed RNA polymerase subunit beta C-terminal
FT                   section"
FT                   /id="PRO_0000308258"
SQ   SEQUENCE   880 AA;  101672 MW;  C67813FAFAADA36A CRC64;
     MNFQFYFLIK HNFISCKLET PLQLLLTTYV NLLKHKKNII PLNVDKARDV YRAHENSYFL
     DHYIRSNQAT CITQRPSVKL GEWVQKGDFL CDTTASSAGE LALGKNILVA YMPWHGYNFE
     DAILVNETLI IYDIYTSLHL EKFEIEIENT NWGPEIIKLS DWMFSDKRDS LYGKELFTQE
     TTKQTYVKEK QITIGVLKQA FLRRKGKLLI NRRFISKIHK YEFLTNQRFV VPQFRYQQTL
     FQQINKNPKK QLISRSIIEN SNQLSIYNKP KIRFTNNVIS SKLFKPLIIF SQVTLLNILS
     QCHIDKSQYQ PTSELLSSKL VAKEQIHELL FKQPKKTGVK IKLKHGTSHT TDFYNKDLLS
     NTKMRAGGEN HRPGESKIYL GSKASVKFIN MNFQEKKTNG YKKISLYNNK TSSQNNVNKK
     SFFLFLNKSI LFLNKPRPKY KSTRPVNVSL NKSAFIYRRS FRTLFPYNFK NMRLSCEKLF
     VNNNNWLINY IKSLDVVIGG EIYRPGNKLQ INLEYLNFKY GFVQKNNYDH LDNDGIIKLG
     TWVKPGDIIV SKLRPIGPHN PTPLEKLVAA ILKKKFDDYK NAAFYTPKDV YGRIVGIEIL
     EPKNLPSDVE YSGIGRVEFY LVDKRRIFVG DKMAGRHGNK GIISNILPKQ DMPYLPDGTP
     VDMVLNPLGV PSRMNVGQVF ETLLGLAGKY LHQHFKVTPF DEVYGPEASR SLVYSKLYEA
     RLKTDQNWIF DPKSPGKTRI FDGQTGANFD QAVTAGYAYM LKLIHLVDHK IHARSTGPYA
     LVTQQPVRGR SRAGGQRLGE MEFWALEAFG ASYNLQEMMT IKSDDILGRT QVFDSILNSK
     PIQNSHPESF RVFINELRSL GVNFQSQILH QYNPNIKTKI