ID   RPOB2_STIHE             Reviewed;        1080 AA.
AC   Q06SF4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta C-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta C-terminal section;
DE            Short=RNA polymerase subunit beta C-terminal section;
GN   Name=rpoB2; Synonyms=rpoBb;
OS   Stigeoclonium helveticum (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   OCC clade; Chaetophorales; Chaetophoraceae; Stigeoclonium.
OX   NCBI_TaxID=55999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 441;
RX   PubMed=16944205; DOI=10.1007/s00438-006-0156-2;
RA   Belanger A.-S., Brouard J.-S., Charlebois P., Otis C., Lemieux C.,
RA   Turmel M.;
RT   "Distinctive architecture of the chloroplast genome in the chlorophycean
RT   green alga Stigeoclonium helveticum.";
RL   Mol. Genet. Genomics 276:464-477(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In S.helveticum the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ630521; ABF60213.1; -; Genomic_DNA.
DR   RefSeq; YP_764412.1; NC_008372.1.
DR   AlphaFoldDB; Q06SF4; -.
DR   GeneID; 4308441; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.10.28.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR036844; Hint_dom_sf.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 2.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   SUPFAM; SSF51294; SSF51294; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1080
FT                   /note="DNA-directed RNA polymerase subunit beta C-terminal
FT                   section"
FT                   /id="PRO_0000308262"
SQ   SEQUENCE   1080 AA;  123464 MW;  E3B5D9BABC3C2FE4 CRC64;
     MSTSQSLNII PIEYSLQTYQ RSNQDTSMLH RPVVKEGEWV QAGDLLSDCA SSIGGEFSIG
     QNILIAYLPW EGYNYEDAIL ISERLVYDDL YTSIHIERYD ISTEKNPYGI EKITKDIVLL
     KDTTELNHLD KNGIAQLGAW LKEGDILVGK ITPTESKKEV ARYVQLYNDI LGKKINYAIR
     DSSLRVPRGL EAKVIRVKTF PERKNETKNW ENKIGLKSKA LAFSVQHFLS KNQSNKKRTL
     SLKSKWEKET SLSSFSSLAW QKKSPLFFVF KNGIFEKLKN FPKKKKKNER VFALKKKSSK
     KFRFFELQKK FRFFFRNFFF FKAPKKKSPL FFFFKSETFD KERNFQKMSP NQSKQNWVKI
     SIKKEWQKVG SFGKKKASKG KISFFPSFPQ ASLVGKASFF SPSRFEKKRD ASNFLKSSCI
     SSVHIYLAEK RKVQVGDKMA GRHGNKGIIS QILPRQDMPY LPDGTPIDMA LNPLGVPSRM
     NVGQIYECLL GLAGKHLGEQ YRIQPFDEAF GPEASRSFVF SKLYSAKTKT GQSWLFQPTN
     PGKLKLFDGR TGNCFDQAIT TGYSYMIKLV HLVDEKIHCL TVDHEVLTTK GWIPLNKVKT
     SHFVATLKKN GQLVYQNPTN IYHYPEFKGE LYHIKNVNLD LLVTLNHRMY VKNGIIEATS
     SVDYQLIPAK DIVGQHKKYC KTAFWDKENY QFILPSVISN SIVIPEKTMN MEAWLQFFGI
     WIAEGWALTN TISNNNVTNF NQSSSSPYVV QISIKKKKVL EILNNVIPIL GYSFNYYDNN
     ITICDKQLWA YLRPLSLGNP YRKLPIWVWD LSQDQARVLL LAMITVFKNG TNSKWEKAAS
     LSSRLSLASV FPEFLKKRID KGLSYYTSSV ELADDISRLA LHAGWSGNNY LLKKKGSISS
     FDGKQIICQF DIWRISIIQS KNQPAVNHGY HSKGKEEVLP YQGAVYCLSV PNEIFYVRRN
     GLSVWTGNSR SSGPYSLITQ QPLKGRSKHG GQRLGEMEVW AIEAYGAAFT LLELLTIKSD
     DVTGRLTIWD YVLYKKPLYI GTPASFKVLI CELQALCLDI GIYKADKSNI LKQINVSSMG