ID   RPOB2_TETOB             Reviewed;         565 AA.
AC   Q1KVX5;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta C-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta C-terminal section;
DE            Short=RNA polymerase subunit beta C-terminal section;
GN   Name=rpoB2; Synonyms=rpoBb;
OS   Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX   NCBI_TaxID=3088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 393;
RX   PubMed=16638149; DOI=10.1186/1471-2148-6-37;
RA   de Cambiaire J.-C., Otis C., Lemieux C., Turmel M.;
RT   "The complete chloroplast genome sequence of the chlorophycean green alga
RT   Scenedesmus obliquus reveals a compact gene organization and a biased
RT   distribution of genes on the two DNA strands.";
RL   BMC Evol. Biol. 6:37-37(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In S.obliquus the gene for this protein is split in two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ396875; ABD48231.1; -; Genomic_DNA.
DR   RefSeq; YP_635949.1; NC_008101.1.
DR   AlphaFoldDB; Q1KVX5; -.
DR   SMR; Q1KVX5; -.
DR   GeneID; 4099816; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..565
FT                   /note="DNA-directed RNA polymerase subunit beta C-terminal
FT                   section"
FT                   /id="PRO_0000308260"
SQ   SEQUENCE   565 AA;  64666 MW;  5105CACA5A6EC040 CRC64;
     MKKKSFCLNF RANKKKKNFV LSKKNIFGKT QKFKICVPFF TYTENKINTH ISPFDSFSCL
     FASQSIESLQ NQKFSKYSLQ KYQRSNQDTL IVQRPMVREG DWVQSGDLLA DCASSLGGEL
     CLGKNIFIAY MPWEGYNYED AILISQRLVT EDVYTSVHIE SYEVETKNTK LGKEQITNKI
     PDIPEKEKNH LDERGIVKLG TFVHEGQILV GKVTPIQKKY RSGYEKLLYT ILEKELIPMR
     DSSLRTPKGL KAKVVEIKIL NFSWNFAPTE KTLPSPIQQK KKNSKILLKK QRKPERVQVY
     LAEKRKIQVG DKMAGRHGNK GIVSKILPIQ DMPFLPDGTP LDMVLNPLGV PSRMNVGQIY
     ECLLGLASKY LNHYYRIQAF DEIYGPHASR SFTFAKLYEA RLKTNQKWLF NPCYPGKMQI
     FDGQTGEPYD SPVTVGIAYM LKLVHLVDDK IHARSTGPYS LVTQQPLRGR SKYGGQRLGE
     MEVWAIEAYG AAFILLEMLT IKSDDISGRM TLWSNILLNM PIYIGTPESF KVLICELQAL
     CLDMGLFQLN KKGFLTQIEH LMQLP