RPOB_ACET2
ID RPOB_ACET2 Reviewed; 1250 AA.
AC A3DIZ4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Cthe_2724;
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000568; ABN53923.1; -; Genomic_DNA.
DR RefSeq; WP_020457900.1; NC_009012.1.
DR AlphaFoldDB; A3DIZ4; -.
DR SMR; A3DIZ4; -.
DR STRING; 203119.Cthe_2724; -.
DR EnsemblBacteria; ABN53923; ABN53923; Cthe_2724.
DR KEGG; cth:Cthe_2724; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_9; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1250
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300303"
FT REGION 1215..1250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1250 AA; 139956 MW; 720D2122326EAFDA CRC64;
MVHPVKLGRN VRMSYSKIDE VIDMPNLIEI QKNSYEQFLK EGFKEVFKDV NPITDYTGNL
ILEFVDYSLD EPPKYSVDEC KERDATYAAP LKVKVRLINK ETGEVKEQEI FMGDFPLMTE
TGTFIINGAE RVIVSQLVRS PGIYYAMKID KAGKQLFSNT VIPNRGAWLE YETDSNDVLS
VRIDRTRKLP LTVLVRALGY GTDLEITELF GEDERILATI QKDSTKTEEE GLLEIYKRLR
PGEPPTVESA KALLHGLFFD PKRYDLAKPG RFKFNKKLSI AARIHGFIAG ENIKDPDTGE
IIVAEGETIS REKAETIQNA GVNTVILRVD GKNVKVIGND MVDIKRYVDF DPKEIGINEK
VKRDVLMEIL EEYKGKGDDA IKKALQERID DLIPKHITKE DIISSISYII GLSYGIGSTD
DIDHLGNRRL RSVGELLQNQ FRIGLSRMER VVRERMTIQD LDVVTPQALI NIRPVAAAIK
EFFGSSQLSQ FMDQTNPLAE LTHKRRLSAL GPGGLSRERA GFEVRDVHHS HYGRMCPIET
PEGPNIGLIG SLSTYARVNE YGFIETPYRK VSKEEPGKVT NEIVYLTADE EDEYIIAQAN
EPLDEEGRFI SNKVVCRYKE EFIEVDPSKI DFMDVSPKQI VSVATSMIPF LENDDANRAL
MGANMQRQAV PLIKTESPIV GTGIEYRAAR DSGVVILAKN PGVVEKVTAN EIIIRTKDGK
RDTYKLLKYM RSNQGTCINQ RPIVKKGEEV EAGDVIADGP STDNGEIALG KNVLVGFMTW
EGYNYEDAIL ISERLVKDDV FTSIHIEEYE AEARDTKLGP EDITREIPNV SEDALKDLNS
EGIIRIGAEV RAGDILVGKV TPKGETELTA EERLLRAIFG EKAREVRDTS LRVPHGESGI
VVDVKIFTRE NGDELAPGVN KLVRVYVAQK RKISVGDKMA GRHGNKGVIS RILPVEDMPF
LPDGTPLDIV LNPLGVPSRM NIGQVLEVHL GYAAKALGWK VATPVFDGAT EEDIVQTLRK
AGLAEDGKSI LYDGRTGEPF ENRVTVGYMY MLKLAHLVDD KIHARSTGPY SLVTQQPLGG
KAQFGGQRFG EMEVWALEAY GAAYTLQEIL TVKSDDVVGR VKTYEAIVKG ENVPEPGIPE
CFKVLIKELQ SLCLDVKVYS EEQEEIAIKE SVEDDLEELN VNIEGREDEV NFNEFNDIGE
EITDEDLEVE DFDLQDLNDD DINPDDTIDA ELDDNLFDDD FDDTFDDDDL
