RPOB_ACHLI
ID RPOB_ACHLI Reviewed; 1271 AA.
AC A9NEL7;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=ACL_0171;
OS Acholeplasma laidlawii (strain PG-8A).
OC Bacteria; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae;
OC Acholeplasma.
OX NCBI_TaxID=441768;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PG-8A;
RX PubMed=21784942; DOI=10.1128/jb.05059-11;
RA Lazarev V.N., Levitskii S.A., Basovskii Y.I., Chukin M.M., Akopian T.A.,
RA Vereshchagin V.V., Kostrjukova E.S., Kovaleva G.Y., Kazanov M.D.,
RA Malko D.B., Vitreschak A.G., Sernova N.V., Gelfand M.S., Demina I.A.,
RA Serebryakova M.V., Galyamina M.A., Vtyurin N.N., Rogov S.I., Alexeev D.G.,
RA Ladygina V.G., Govorun V.M.;
RT "Complete genome and proteome of Acholeplasma laidlawii.";
RL J. Bacteriol. 193:4943-4953(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000896; ABX80797.1; -; Genomic_DNA.
DR RefSeq; WP_012242128.1; NC_010163.1.
DR AlphaFoldDB; A9NEL7; -.
DR SMR; A9NEL7; -.
DR STRING; 441768.ACL_0171; -.
DR PRIDE; A9NEL7; -.
DR EnsemblBacteria; ABX80797; ABX80797; ACL_0171.
DR GeneID; 66293168; -.
DR KEGG; acl:ACL_0171; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_14; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000008558; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 3.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1271
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000086360"
SQ SEQUENCE 1271 AA; 141439 MW; 9B7806A44C6FB33F CRC64;
MSYKTVKYGK KAERRDYSKM LHQVELPNLI EIQTKSFEEF VETGIDELLK DISPIEGHNG
DLKLYFEESS LSEPKYSTIE TKIRDLNYSR QLSARVKLEN AITGEVREST VLMTDLPMMT
PSGTFIINGA ERVVVSQIVR SSGVYYTSEL DKKINQIKYS AQVIPTRGAW IEFEQGSKEI
LYAKLDRSKK VPLTTFIRAL GFTTKKDIEE TFGRSSLITN TFEKDETKSP NEAVIDLYSK
LRQGEKVPAD AAREFIRMRL FDARRYDLAK VGRYKFIKKL DVLARAEGTY LANDIILDGE
VLVAKDTHLT KEVIQILAQN RDAFRKQLIT KENNLQNETA DEILATTLPE GGNTLYAKEN
VVNLKTGEVL VKKNEAITEE VLTNLRKNRH SIDEKVIKYF LTEDVYKKES LRQGVISEIL
DVYVYDDAGD KSNVIRIIGS DQREDRIFVA VSDIVASISY FLNLYDGLGN VDDIDHLGNR
RLRLIGELLK NQFRIGLARA EKNIKDKMST TNFNDATPAN VINMTPLVGA IKTFFGSSQL
SQFMDQINPL AELTQKRRVS ALGTGGIARD RAGVEVRDVH NSHYGRLCPI ETPEGPSIGL
ISSLATYAKV DEYGFIKTPF LKVVQNGNET SVTKEVIYLT ADEESDHVIA SAATPMDEHG
LFIESRVIAR RNGETGIYDA NEITAMDVSP KQVVSVATSS IPFLEHDDAS RALMGANMQR
QAVPLLQPTS PIVGTGVEYR AAKDSGAVVI AKNSGYVTYV DGKKIIITPE PTDTIKSGSK
TLYKVGGEFD YGIAKKLYET KSVQNDQYDL VQFFRSNQDT LILQKPIVMQ GEYVDAGDVI
ADGPSTENGE LALGRNVTVA FMTWEGYNYE DAVIMSEDLV KHDVYTSIHI DEYEIETRDL
KQANGKEEIT REIPNVGQEA IKFLDERGII VPGSEVKEGD ILVGKITPKG VFEPTPSEKL
IHAVIGDKAK EYRDSSLRVP HGGGGVVQSV QYFSKKNGDQ LPTGVNEQIR VYIAKKRKIT
EGDKMAGRHG NKGVISKILP REDMPYLADG TYVDIMLNPL GVPSRMNIGQ ILEIHLGISA
QKLGIKVASP VFDGVTNDEL GAIMEEAGIA PDGKTTLYDG RTGEPYNNRI SVGVMYMIKL
SHMVDDKLHA RNVGPYTLVT QQPMGGKAQN GGQRFGEMEV WALYAYGAAH TLQEMLTIKS
DDMIGRNKVY YAITHGQEIP KASIPESFRV LTRELQALGL YVELIDANKG ENEAIKSLVD
NSSKGFNKGR F
