ID   RPOB_ACOAM              Reviewed;        1083 AA.
AC   A9LYH7;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Acorus americanus (Sweetflag) (Acorus calamus var. americanus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Acoraceae; Acorus.
OX   NCBI_TaxID=263995;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Peery R.M., Chumley T.W., Kuehl J.V., Boore J.L., Raubeson L.A.;
RT   "The complete chloroplast genome of Acorus americanus.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; EU273602; ABX38736.1; -; Genomic_DNA.
DR   RefSeq; YP_001586174.1; NC_010093.1.
DR   AlphaFoldDB; A9LYH7; -.
DR   SMR; A9LYH7; -.
DR   GeneID; 5777712; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1083
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329197"
SQ   SEQUENCE   1083 AA;  122370 MW;  8F22C50244A37BC8 CRC64;
     MGFLFSINRK LKMPRDGNEG MFTIPGFSQI QFEGFCRFID QGLMEEFHKF PKIEDTDQEI
     EFQLFVERYQ LVEPLIKERD AVYESLTYSS ELYVPAGLIW KTGRDMQEQT IFIGNIPLMN
     SLGTFIVNGI YRIVINQILQ SPGIYYRSEL DHNGISVYTS TIISDWGGRS ELEIDRKSRI
     WARVSRKQKI SILVLSSAMG SNLREILDNV CYPEIFLSFL NDREKKKIGS KENAILEFYQ
     QFACVGGDPV FSESLCKELQ KKFFQQRCEL GRIGRRNMNR RLNLDIPQSN TFLLPRDVLA
     AADHLIGMKF GMGTLDDMNH LKNKRIRSVA DLLQDQFGLA LVRLENAVRG TICGAIRHKL
     ILTPQNLVSS TSLTTTYESF FGLHPLSQVL DRTNPLTQIV HGRKLSYLGP GGLTGRTASF
     RIRDIHPSHY GRICPIDTSE GINVGLIGSL AIHARIGHWG SIESPFYEVY QRSKETKMVF
     LSPSRDEYYT VATGNSLALN RGGIQEEQIV PARYRQEFLT IAWEQIHLRS IFPFQYFSIG
     ASLIPFIEHN DANRALMSSN MQRQAVPLSR SEKCIVGTGL ERQAALDSGV SAIAECEGKI
     IHTDTHKIVL SGHGDTISIP LVMYQRSNKN TCMHQNPQVR RGKCIKKGQI LADGAATVGG
     ELALGKNVLV AYMPWEGYNF EDAVLISERL VYEDIYTSFH IRKYEIQTHV TSQGPERITH
     EIPHLEAHLL RNLDRNGIVA LGSWVETGDI LVGKLTPQTA NESSYAPEDR LLRAILGIQV
     STAKETCLKL PIGGRGRVID VRWIQKKGGS SYNPETIRVY ISQKREIKVG DKVAGRHGNK
     GIISKILSRQ DMPYLQDGTP VDMVFNPLGV PSRMNVGQIF ECSLGLAGDL LDRHYRIAPF
     DERYEQEASR KLVFSELYEA SKQTANPWVF EPEYPGKSRI FDGRTGDPFE QPVLIGKSYI
     LKLIHQVDDK IHGRSSGHYA LVTQQPLRGR AKQGGQRVGE MEVWALEGFG VAHILQEMLT
     YKSDHIRARQ EVLGTTIIGG TIPTPEDAPE SFRLLVRELR SLALELNHFL VSEKNFQINR
     KEA