RPOB_ADICA
ID RPOB_ADICA Reviewed; 1071 AA.
AC Q85FM7;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Adiantum capillus-veneris (Maidenhair fern).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Polypodiopsida; Polypodiidae; Polypodiales; Pteridineae; Pteridaceae;
OC Vittarioideae; Adiantum.
OX NCBI_TaxID=13818;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12755170; DOI=10.1093/dnares/10.2.59;
RA Wolf P.G., Rowe C.A., Sinclair R.B., Hasebe M.;
RT "Complete nucleotide sequence of the chloroplast genome from a
RT leptosporangiate fern, Adiantum capillus-veneris L.";
RL DNA Res. 10:59-65(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND RNA EDITING.
RC TISSUE=Frond;
RX PubMed=15363849; DOI=10.1016/j.gene.2004.06.018;
RA Wolf P.G., Rowe C.A., Hasebe M.;
RT "High levels of RNA editing in a vascular plant chloroplast genome:
RT analysis of transcripts from the fern Adiantum capillus-veneris.";
RL Gene 339:89-97(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- RNA EDITING: Modified_positions=43 {ECO:0000269|PubMed:15363849}, 72
CC {ECO:0000269|PubMed:15363849}, 77 {ECO:0000269|PubMed:15363849}, 142
CC {ECO:0000269|PubMed:15363849}, 215 {ECO:0000269|PubMed:15363849}, 240
CC {ECO:0000269|PubMed:15363849}, 285 {ECO:0000269|PubMed:15363849}, 321
CC {ECO:0000269|PubMed:15363849}, 330 {ECO:0000269|PubMed:15363849}, 540
CC {ECO:0000269|PubMed:15363849}, 729 {ECO:0000269|PubMed:15363849}, 759
CC {ECO:0000269|PubMed:15363849}, 787 {ECO:0000269|PubMed:15363849}, 879
CC {ECO:0000269|PubMed:15363849}, 905 {ECO:0000269|PubMed:15363849}, 994
CC {ECO:0000269|PubMed:15363849}, 1049 {ECO:0000269|PubMed:15363849}, 1060
CC {ECO:0000269|PubMed:15363849}; Note=The nonsense codons at positions
CC 43, 77, 240 and 330 are modified to sense codons.;
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY178864; AAP29384.3; -; Genomic_DNA.
DR RefSeq; NP_848052.1; NC_004766.1.
DR AlphaFoldDB; Q85FM7; -.
DR SMR; Q85FM7; -.
DR GeneID; 807460; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 3.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW RNA editing; Transcription; Transferase.
FT CHAIN 1..1071
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000048008"
SQ SEQUENCE 1071 AA; 120827 MW; 9C8F46D4975950DB CRC64;
MPVLPELAQI QFKGFSRFVH QKLLKELENF PKIEDTDKEV EFRSIGNQYQ LTEPSLEERD
AAYQCITYSA DLYVPAQLIC KEDQIVQEED VRLGSIPWMN SGGTFIINGI ARVLVSQILR
SPGIYYNRES DQRGILAYTS TVISDQGGRF KLEIDGRNRI WVRISKKKKI SIFILLVAMG
LCKRDILQNS RYPRIFSNMF KRQKESIESP EDAIVELYKH LYSATDADVF FSESIFRELQ
KRFFQHRCEL GRIGRRNLNI KLTLDVPETE SFLLPQDILA AADHLVEISY GEGKLDDIDH
LKNRRVRSVA DLLQEQLKLA LNRLENLIRQ NLSRAVRRKR AITPRGLVTP VPVIAAFKEF
FGSHPLSQFL DQTNPLSEMV HKRRLSSVGP GGLTRRTASF QARDIHFSHY GRICPIETSE
GMNAGLISSL AIQAGVNTSG SLGSPYLEMS DSSGEEQLVN LSPAEDDYYR VASENLLLSE
WRGSEKEIIP VRYQQEFLSV LWEQVDFRSI HPLHHFSIGA SLIPFIEHND ANRALMGSTM
QRQAVPLIKP ERCIVGTGLE SQVALDSGSV AISEQDGKVC YIDGNRIELS LIHEAKKNKL
AQPTTIEELK IFERSNNNTC MHQKPAVSLG ELLRGGEIVA DGAATVRGEL ALGKNILVAY
MPWEGYNFED AVLISERLIY EDIFTSLHIE KHEVEICATN QGPERVTKQI SQLDGYLLRH
LDDDGLVELG SWVEAGDVLV GKLTPQEGAS SSRVPEGRLL QAIFGIQLVN ARESCLRVPI
GGRGRVIDVR WVYPEDDATN DLEVIHIYIL QRRKIQVGDK IAGRHGNKGI VSKIVPRQDM
PYLQNGTPVD MILSPLGVPS RMNVGQIFEC LLGLAGEFLE THYRVVPFDE RYEREASRKL
VFAELHEAGA RTNNPWLFEP SHPGKSRLID GRTGDPFGQS ITTGKAYIMK LIHQVDDKIH
ARSSGPYALV TQQPLKGKSR RGGQRIGEME VWALEGFGVS YTLQEMLTTK SDHIQARYKA
LSAIMTGKPV CKPKTVPESF RLLVRELRCM GLNLERNFIS EEDLGREFEN I