RPOB_AERHH
ID RPOB_AERHH Reviewed; 1342 AA.
AC A0KQA5;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=AHA_4028;
OS Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=380703;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC
RC 2358 / NCIMB 9240 / NCTC 8049;
RX PubMed=16980456; DOI=10.1128/jb.00621-06;
RA Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H.,
RA Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL J. Bacteriol. 188:8272-8282(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK39764.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000462; ABK39764.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_010635847.1; NC_008570.1.
DR RefSeq; YP_858456.1; NC_008570.1.
DR AlphaFoldDB; A0KQA5; -.
DR SMR; A0KQA5; -.
DR STRING; 380703.AHA_4028; -.
DR PRIDE; A0KQA5; -.
DR EnsemblBacteria; ABK39764; ABK39764; AHA_4028.
DR GeneID; 64360556; -.
DR KEGG; aha:AHA_4028; -.
DR PATRIC; fig|380703.7.peg.3988; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_6; -.
DR Proteomes; UP000000756; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1342
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300273"
SQ SEQUENCE 1342 AA; 150227 MW; C80B77351EA0E5DA CRC64;
MVYSYTEKKR IRKDFGKRDQ VLDTPYLLSI QLDSFKQFIE ADPEGEYGLE AAFRSVFPIT
SYSGSAELQY VSYRLGEPVF DVKECQIRGV TYSAPLRVKL RMVLYDREAA AGTVKDIKEQ
EVYMGEIPLM TENGTFVING TERVIVSQLH RSPGVFFDHD KGKTHSSGKV LYNARVIPYR
GSWLDFEFDA KDNLFVRIDR RRKLPASIIL RALDFSSEQI LATFFETIGF EVKDGKLMMD
LVPERLRGET ATFDIVANGA VVVETGRRVT ARHIRQLEKD SVTQIEVPVE YVVGKVAAKD
YAHPQTGEMV VTANQALSLE AVANLSQAGF KHFEVLFTNE LDHGAYMSET LRIDSSSSRL
EALVEIYRMM RPGEPPTREA AEQLFENLFF SSERYDLSTV GRMKFNRRLA REDETGVGTL
TKDDIVDVMK RLIDIRNGND EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS
LGDLDTLMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR
ERAGFEVRDV HPTHYGRLCP IETPEGPNIG LINSLSVYSR TNEYGFLETP YRKVIDGVIT
DEVDYLSAIE EGKYVIAQAN AATTEDGRLK DELIPCRHKG ESTFMNADQI QYMDVSPQQI
VSVAAALIPF LEHDDANRAL MGSNMQRQAV PTLRADKPLV GTGMERAVAV DSGVTVVAKR
GGMIDYVDAS RIVIKVNEDE LLPGEAGIDI YSLTKYTRSN QNTCINQRPC VMLGEPVMAG
DVLADGPSTD LGELALGQNL RVAFMPWNGY NFEDSILVNE RVVQEDRLTT IHIQELACIS
RDTKLGPEEI TADIPNVGEA ALSKLDESGI VYVGAEVKGG DILVGKVTPK GETQLTPEEK
LLRAIFGEKA SDVKDSSLRV PNGVYGTVVD VQVFTRDGVE KDKRAKEIEE MQLKEAKKDL
TEEFKILEDG IFGRSRNLLL AAGYSEDRLN KLDRSKWFEL AIEDEAKQIE LEQIAEQHVE
LKAEFDKKFE NKRRKIIQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIC
PVEDMPHDEY GRPVDIVLNP LGVPSRMNIG QILEVHLGLA AKGIGEKIDR MVKEQRELHE
MRDFLQQVYD LGEKDTQQVN VAELSDDDVR TLVGNLRKGL PVATPVFDGA KEREIKALLK
LADLPESGQI ALFDGRTGNA FERKVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG
GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYKNIVD GDHRMEPGMP
ESFNVLLKEI RSLGINIELD EE
