RPOB_AERS4
ID RPOB_AERS4 Reviewed; 1342 AA.
AC A4SHU9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=ASA_0283;
OS Aeromonas salmonicida (strain A449).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=382245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A449;
RX PubMed=18801193; DOI=10.1186/1471-2164-9-427;
RA Reith M.E., Singh R.K., Curtis B., Boyd J.M., Bouevitch A., Kimball J.,
RA Munholland J., Murphy C., Sarty D., Williams J., Nash J.H., Johnson S.C.,
RA Brown L.L.;
RT "The genome of Aeromonas salmonicida subsp. salmonicida A449: insights into
RT the evolution of a fish pathogen.";
RL BMC Genomics 9:427-427(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABO88471.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000644; ABO88471.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_005318568.1; NC_009348.1.
DR AlphaFoldDB; A4SHU9; -.
DR SMR; A4SHU9; -.
DR STRING; 382245.ASA_0283; -.
DR EnsemblBacteria; ABO88471; ABO88471; ASA_0283.
DR KEGG; asa:ASA_0283; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_6; -.
DR Proteomes; UP000000225; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1342
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300274"
SQ SEQUENCE 1342 AA; 150252 MW; 200AF1BC1ECAB2D8 CRC64;
MVYSYTEKKR IRKDFGKRDQ VLDTPYLLSI QLDSFKQFIE ADPEGEYGLE AAFRSVFPIT
SYSGSAELQY VSYRLGEPVF DVKECQIRGV TYSAPLRVKL RMVLYDREAA AGTVKDIKEQ
EVYMGEIPLM TENGTFVING TERVIVSQLH RSPGVFFDHD KGKTHSSGKV LYNARVIPYR
GSWLDFEFDA KDNLFVRIDR RRKLPASIIL RALDFSSEEI LANFFETIGF EVKDGKLMMD
LVPERLRGET ATFDIVANGA VVVETGRRVT ARHIRQLEKD AVTQIEVPVE YVVGKVAAKN
YTHPQTGEMV VTANQALSLE AVANLSQAGF KHFEVLFTNE LDHGAYMSET LRIDSSSSRL
EALVEIYRMM RPGEPPTREA AEQLFENLFF SSERYDLSTV GRMKFNRRLS REDETGAGTL
TKDDIVEVMK RLIDIRNGND EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVKERLS
LGDLDTLMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR
ERAGFEVRDV HPTHYGRLCP IETPEGPNIG LINSLSVYSR TNEYGFLETP YRKVIDGVIT
DEVDYLSAIE EGKYVIAQAN AATTEDGRLK DELIPCRHKG ESTFMNADQI QYMDVSPQQI
VSVAAALIPF LEHDDANRAL MGSNMQRQAV PTLRADKPLV GTGMERAVAV DSGVTVVAKR
GGMIDYVDAS RIVIKVNEDE LLPGEAGIDI YSLTKYTRSN QNTCINQRPC VMLGEPVMAG
DVVADGPSTD LGELALGQNL RVAFMPWNGY NFEDSILVNE RVVQEDRLTT IHIQELACIS
RDTKLGPEEI TADIPNVGEA ALSKLDESGI VYVGAEVKGG DILVGKVTPK GETQLTPEEK
LLRAIFGEKA SDVKDSSLRV PNGVYGTVVD VQVFTRDGVE KDKRAKEIEE MQLKEAKKDL
TEEFKILEDG IFGRSRNLLL AAGYSEDRLN KLDRTKWFEL AIEDEAKQIE LEQIAEQHIE
LKADFDKKFE NKRRKIIQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIC
PVEDMPHDEY GRPVDIVLNP LGVPSRMNIG QILEVHLGLA AKGIGEKIDR MIKEQRALHE
MRDFLQQVYD LGEKDTKQVD IAELSDDDVR TLVGNLRKGL PVATPVFDGA KEHEIKALLK
LADLPESGQI SLFDGRTGNV FERKVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG
GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG RTKMYKNIVD GDHRMEPGMP
ESFNVLLKEI RSLGINIELD EE
