ID   RPOB_AGRFC              Reviewed;        1378 AA.
AC   Q8UE08;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-JAN-2004, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Atu1956;
GN   ORFNames=AGR_C_3569;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE007869; AAK87716.2; -; Genomic_DNA.
DR   PIR; AB2817; AB2817.
DR   PIR; C97595; C97595.
DR   RefSeq; NP_354931.2; NC_003062.2.
DR   RefSeq; WP_006316439.1; NC_003062.2.
DR   AlphaFoldDB; Q8UE08; -.
DR   SMR; Q8UE08; -.
DR   STRING; 176299.Atu1956; -.
DR   EnsemblBacteria; AAK87716; AAK87716; Atu1956.
DR   GeneID; 66222170; -.
DR   KEGG; atu:Atu1956; -.
DR   PATRIC; fig|176299.10.peg.1968; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   PhylomeDB; Q8UE08; -.
DR   BioCyc; AGRO:ATU1956-MON; -.
DR   Proteomes; UP000000813; Chromosome circular.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1378
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047850"
SQ   SEQUENCE   1378 AA;  153645 MW;  4C1D8C8DCFE4993F CRC64;
     MAQTLSFNGR RRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVDEPKGGR PDEGLNAVFK
     SVFPITDFSG ASMLEFVSYE FEAPKFDVEE CRQRDLTYAA PLKVTLRLIV FDIDEDTGAK
     SIKDIKEQSV YMGDMPLMTN NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KSHSSGKLLF
     AARVIPYRGS WLDIEFDAKD IVYARIDRRR KLPVTSLLMA LGMDGEEILS TFYTKATYER
     SGDGWRIPFQ PEALKNAKVI TDMIDADTGE VVVEGGKKLT PRLIRQLVDK GLKALKATDE
     DLYGNYLAED IVNYSTGEIY LEAGDEIDEK TLGLILQSGF DEIPVLNIDH VNVGAYIRNT
     LSADKNQNRQ EALFDIYRVM RPGEPPTMDS AEAMFNSLFF DAERYDLSAV GRVKMNMRLD
     LDAEDTVRTL RKEDILAVVK MLVELRDGKG EIDDIDNLGN RRVRSVGELM ENQYRLGLLR
     MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQVNP LSEITHKRRL
     SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP
     YRKIIDGKVT TDVIYLSAME EAKYYVAQAN AELDGEGAFT EEFVVCRHSG EVMLAPRDNI
     NLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLLRAEAPFV GTGMEPIVAR
     DSGAAIAARR GGVVDQVDAT RIVIRATEDL DAGKSGVDIY RLQKFQRSNQ NTCVNQRPLV
     SVGDAISKGD IIADGPSTDL GDLALGRNAL VAFMPWNGYN YEDSILMSER IVSDDVFTSI
     HIEEFEVMAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVQPGD ILVGKITPKG
     ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTFGTIVEV RVFNRHGVEK DERAMAIERE
     EIERLAKDRD DEQAILDRNV YGRLIDMLRG HVSIAGPKGF KKGVELSNAV VSEYPRSQWW
     MFAVEDEKAQ SELEALRGQY DESKSRLEQR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR
     KIQPGDKMAG RHGNKGVVSR IVPVEDMPFL EDGTHVDICL NPLGVPSRMN VGQILETHLA
     WACAGMGKKI GEMLEEYRKT MDISELRSEL TEIYASEAND EVQRFDDDSL VKLAEEAKRG
     VSIATPVFDG AHEPDVAAML KKAGLHESGQ SVLYDGRTGE PFDRKVTVGY MYMIKLNHLV
     DDKIHARSIG PYSLVTQQPL GGKAQFGGQR FGEMEVWALE AYGAAYTLQE MLTVKSDDVA
     GRTKVYEAIV RGDDTFEAGI PESFNVLVKE MRSLGLSVEL ENSKIENQSE DQLPDAAE