ID   RPOB_AGRVS              Reviewed;        1379 AA.
AC   B9JVM8;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Avi_1826;
OS   Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain
OS   S4)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=311402;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S4 / ATCC BAA-846;
RX   PubMed=19251847; DOI=10.1128/jb.01779-08;
RA   Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K.,
RA   Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L.,
RA   Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A.,
RA   Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M.,
RA   Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N.,
RA   Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W.;
RT   "Genome sequences of three Agrobacterium biovars help elucidate the
RT   evolution of multichromosome genomes in bacteria.";
RL   J. Bacteriol. 191:2501-2511(2009).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000633; ACM36308.1; -; Genomic_DNA.
DR   RefSeq; WP_015915731.1; NC_011989.1.
DR   AlphaFoldDB; B9JVM8; -.
DR   SMR; B9JVM8; -.
DR   STRING; 311402.Avi_1826; -.
DR   PRIDE; B9JVM8; -.
DR   EnsemblBacteria; ACM36308; ACM36308; Avi_1826.
DR   KEGG; avi:Avi_1826; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001596; Chromosome 1.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1379
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000165783"
SQ   SEQUENCE   1379 AA;  153824 MW;  D3BA6A065F1A3F13 CRC64;
     MAQTLSFNGR RRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVDEPEGGR PDEGLQTVFK
     SVFPITDFSG ASMLEFVSYE FEPPKFDVDE CRQRDLTYAA PLKVTLRLIV FDIDEDTGAR
     SIKDIKEQSV YMGDMPLMTN NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KSHSSGKLLF
     AARVIPYRGS WLDIEFDAKD IVHARIDRRR KIPVTSLLMA LGMDGEEILS TFYSKSDYKR
     DGKGWRVPFQ AETLKGSKTL VDMVDADSGE VVVEAGKKLT PRLLRQLQDK GLKALKATDE
     DLYGNYLAED IVNFATGEIY LEAGDEIDEK TLPIILDAGF EEIPVLGIDH INVGAYIRNT
     LAADKNENRQ DALFDIYRVM RPGEPPTMDS AEAMFNSLFF DSERYDLSAV GRVKMNMRLD
     LEVADTVRVL RKEDILAVVK MLVELRDGKG EIDDIDNLGN RRVRSVGELM ENQYRLGLLR
     MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQVNP LSEITHKRRL
     SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP
     YRKIIDGVVT KDVIYLSAME EAKYYVAQAN AELNAEGKFV EEFVVCRHAG EVMLSPRDTI
     NLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLLRAEAPFV GTGMEPIVAR
     DSGAAIGARR GGVVDQVDAT RIVIRATEDL DAGKSGVDIY RLQKFQRSNQ NTCVNQRPLV
     AVGDILNKGD IIADGPSTDL GDLALGRNAL VAFMPWNGYN YEDSILMSER IVSEDVFTSI
     HIEEFEVMAR DTKLGPEEIT RDIPNVSEEA LRNLDEAGIV YIGAEVQPGD ILVGKITPKG
     ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTFGTIVEV RVFNRHGVEK DERAMAIERE
     EIERLAKDRD DEQAILDRNV YGRLLDTLRG QVSIAGPKGF KKGVELNNAV ISEYPRSQWW
     MFAVEDEKVQ SELEALRGQY DESKSRLEQR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR
     KIQPGDKMAG RHGNKGVVSR IVPVEDMPFL EDGTHVDIVL NPLGVPSRMN VGQILETHLA
     WACAGMGRKI GQMLEDYQKH LDITELRTEL VDLYASESHD EVARFDDESL LRLADQAKSG
     LSIATPVFDG AHESDVSEML TRAGLHTSGQ SVLYDGRTGE TFDRKVTVGY MYMIKLNHLV
     DDKIHARSIG PYSLVTQQPL GGKAQFGGQR FGEMEVWALE AYGAAYTLQE MLTVKSDDVA
     GRTKVYEAIV RGDDTFEAGI PESFNVLVKE MRSLGLSVEL ENSKLETADT VNPLPDAAE