ID   RPOB_ALIF1              Reviewed;        1342 AA.
AC   Q5E238; Q5E237;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 3.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=VF_2414;
GN   ORFNames=VF2413;
OS   Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=312309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700601 / ES114;
RX   PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA   Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA   Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA   Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT   "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT   pathogenic congeners.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=18366731; DOI=10.1186/1471-2164-9-138;
RA   Mandel M.J., Stabb E.V., Ruby E.G.;
RT   "Comparative genomics-based investigation of resequencing targets in Vibrio
RT   fischeri: focus on point miscalls and artefactual expansions.";
RL   BMC Genomics 9:138-138(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000020; AAW86909.2; -; Genomic_DNA.
DR   RefSeq; WP_011262791.1; NC_006840.2.
DR   RefSeq; YP_205797.2; NC_006840.2.
DR   AlphaFoldDB; Q5E238; -.
DR   SMR; Q5E238; -.
DR   STRING; 312309.VF_2414; -.
DR   PRIDE; Q5E238; -.
DR   EnsemblBacteria; AAW86909; AAW86909; VF_2414.
DR   KEGG; vfi:VF_2414; -.
DR   PATRIC; fig|312309.11.peg.2447; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000537; Chromosome I.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1342
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224119"
SQ   SEQUENCE   1342 AA;  149982 MW;  AD1604C9AF6C5BA1 CRC64;
     MVYSYTEKKR IRKDFGKRPQ VLDIPYLLSI QLDSFTKFIE QDPEGQYGLE AAFRSVFPIQ
     SYNGNSKLEY VSYNLREPEF DVKECQIRGV TYSAPLRVKL RLVVYDKDAP ANTVKDIKEQ
     EVYMGEIPLM TDNGTFVING TERVIVSQLH RSPGVFFDSD KGKTHSSGKV LYNARVIPYR
     GSWLDFEFDP KDNLFVRIDR RRKLPATIIL RALNKTTEEI LDLFFDKVVF EVKDQTLMME
     LIPERLRGET ATFDIEANGK VYVEAGRRIT ARHIRQLTKD DITHIEVPVD YIVGKVASHD
     YVNEDTGEII IAANQEFSLE DLANLSQAGY KKIEVLFTND LDHGAYISDT LRADSTVDRL
     SALVEIYRMM RPGEPPTKEA AEALFESLFF SEERYDLSTV GRMKFNSSIG RDNDEGAGVL
     DETDIIEVMR KLIDIRNGIG EVDDIDHLGN RRIRSVGEMA ENQFRVGLVR VERAVRERLS
     LGDLDAIMPQ DLINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEVTHKRRI SALGPGGLTR
     ERAGFEVRDV HATHYGRLCP IETPEGPNIG LINSLSAFAQ CNEYGFLETP YRRVVDGQVT
     DQVDYLSAIE EGTFVIAQAN AVLTEEGTFA DELIIARQKG ESGLHPREHI QYMDVATNQV
     VSVAASLIPF LEHDDANRAL MGANMQRQAV PTLKADKPLV GTGIERNVAV DSGVTAVAKR
     GGVVQSVDAS RIVIKVNEDE LVPGEAGIDI YNLTKYTRSN QNTCINQRPT VLPGEPVTRG
     DVLADGPSTD LGELALGQNM RIAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELSCVA
     RDTKLGSEEI TADIPNVGEA ALSKLDESGI VYIGAEVKGG DILVGKVTPK GETQLTPEEK
     LLRAIFGEKA SDVKDSSLRV PNSVSGTIID VQVFTRDGVE KDKRALEIEQ MQLKEAKKDI
     TEEFQILEGG LLARVRTLLV AAGVSEVKLD AMDRKQWLEI TLDDEAQQNQ LEQLAEQYDE
     LKAEFDKKFE TKRRKITQGD DLAPGVLKIV KVYLAVKRRI QPGDKMAGRH GNKGVISKIN
     PVEDMPYDEK GQPVDIVLNP LGVPSRMNIG QILEVHMGLA AKGVGDKINQ MLKEQQELHK
     FRNFLQKVYD LGETRQEVDI AALSDDEVRT LIKNLRGGLP IATPIFDGAP EASIKELLKL
     VDLPESGQLK LFDGRTGDAF ERPVTVGYMY MLKLNHLVDD KMHARSTGSY SLVTQQPLGG
     KAQFGGQRFG EMEVWALEAY GAAYTLQEML TVKSDDVNGR TKMYKNIVDG DHRMEPGMPE
     SFNVLLKEIR SLGINIELED EE