ID   RPOB_ALKEH              Reviewed;        1355 AA.
AC   Q0ABI2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Mlg_0451;
OS   Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Alkalilimnicola.
OX   NCBI_TaxID=187272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1101 / DSM 17681 / MLHE-1;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S.,
RA   Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B.,
RA   Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P.;
RT   "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000453; ABI55805.1; -; Genomic_DNA.
DR   RefSeq; WP_011628201.1; NC_008340.1.
DR   AlphaFoldDB; Q0ABI2; -.
DR   SMR; Q0ABI2; -.
DR   PRIDE; Q0ABI2; -.
DR   EnsemblBacteria; ABI55805; ABI55805; Mlg_0451.
DR   KEGG; aeh:Mlg_0451; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001962; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1355
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300276"
SQ   SEQUENCE   1355 AA;  150619 MW;  1B8471DD89C7FC86 CRC64;
     MAYSFTEKKR IRKDFGKLPN ILEVPYLLAT QIDSYRQFLQ EERAAESRED VGLHGAFRSV
     FPITSHSGTA ALEYVSYRIG DPGFDVKECQ SRGLTYAAPL RMLVRLVIYD KDGQSVKDIK
     EQEVYMGELP LMTRNGTFVI NGTERVIVSQ LHRSPGVFFD HDKGKTHSSG KLLFSARVIP
     YRGSWLDFEF DPKDNIYVRI DRRRKLPATI LLRALGFDTQ QILDLFFDKN AFHFRDDGSV
     VLDLVPERLR GETAAFDIKA NGQVLVEAGR RVTARHIREL GKAGVDTLEV PAEYLEGKIV
     AHDVVDTGTG EIICEANTEL TAELVQAIQD AGVKQLDTLF VNDLDRGPYM SQTLAIDPTR
     TQLEALVEIY RMMRPGEPPT KEAAENLFQN LFFSPDRYDL SPVGRMKFNL RVGRDEVTGP
     GVLDNDDILA VLRELINIRN GDGQVDDIDH LGNRRVRCVG EMAENVFRVG LVRVERAVRE
     RLSLAESEGL MPQELINAKP VAAAIKEFFG SSQLSQFMDQ NNPLSEVTHK RRVSALGPGG
     LTRERAGFEV RDVHPTHYGR VCPIETPEGP NIGLINSLAT YARTNRYGFL ETPYRRVENG
     VVTDKVDYLS AIEEARYVIA QANARVGEDG RLMDELISIR HGNEFGMATP DKVQYMDVSP
     KQIVSVAASL VPFLEHDDAN RALMGSNMQR QAVPTLRADK PLVGTGMERA VARDSGVIVT
     AERGGVVEQV DAGRIVVRVN DDETEAGEPG VDIYNLVKYL RSNQNTCMNQ KPLVRPGDVV
     ARGDALADGP STDMGELALG QNMLVAFMPW NGYNFEDSIL ISERVVKEDR YTTIHIEELT
     CVARDTKLGT EEITADIPNV SESALNRLDE AGIVHIGAEV KAGDILVGKV TPKGETQLTP
     EEKLLRAIFG EKASDVKDTS LRVPTGMDGK VIDVQVFTRD GVEKDSRALA IEQAELAAIR
     KDLRDQERIF EDDAFSRLER ALVGQVAEGG PSGLARGAEV TADYLANLDR KRWFEIRMRD
     EEANRQLEAV RAQLEAQRDS LERHFEEKKG KITAGDDLAP GVVKMVKVYL AVRRRLQPGD
     KMAGRHGNKG VISMIVPEED MPFTEDGEPV DVVLSPLGVP SRMNVGQVLE THLGWAARGL
     GKRIGEMLEQ HTKMQELRAF LDQVYNASGQ KVDLDSLDDE EIKGLAFNLR EGVPMATPVF
     DGAQEPEIKN MLELAGLPES GQATLYDGRT GEAFDRPVTI GYMYMLKLNH LVDDKVHARS
     TGPYSLVTQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL QEMLTVKSDD VAGRTKMYKN
     IVDGDHRMEA GMPESFNVLV KEIRSLGINI ELEQD