ID   RPOB_ALKHC              Reviewed;        1180 AA.
AC   Q9Z9M2;
DT   21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BH0126;
OS   Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS   / JCM 9153 / C-125) (Bacillus halodurans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=272558;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX   PubMed=10192928; DOI=10.1271/bbb.63.452;
RA   Takami H., Takaki Y., Nakasone K., Hirama C., Inoue A., Horikoshi K.;
RT   "Sequence analysis of a 32-kb region including the major ribosomal protein
RT   gene clusters from alkaliphilic Bacillus sp. strain C-125.";
RL   Biosci. Biotechnol. Biochem. 63:452-455(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX   PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA   Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA   Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT   "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT   and genomic sequence comparison with Bacillus subtilis.";
RL   Nucleic Acids Res. 28:4317-4331(2000).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AB017508; BAA75263.1; -; Genomic_DNA.
DR   EMBL; BA000004; BAB03845.1; -; Genomic_DNA.
DR   PIR; T44375; T44375.
DR   RefSeq; WP_010896309.1; NC_002570.2.
DR   AlphaFoldDB; Q9Z9M2; -.
DR   SMR; Q9Z9M2; -.
DR   STRING; 272558.10172738; -.
DR   EnsemblBacteria; BAB03845; BAB03845; BAB03845.
DR   KEGG; bha:BH0126; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001258; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1180
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047859"
FT   REGION          1154..1180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1180 AA;  132638 MW;  00E1517AE4D07970 CRC64;
     MTGQLIQYGR HRQRRSYARI NEVLELPNLI EIQTASYQWF LDEGLREMFQ DISPIQDFTG
     NLVLEFIDYS LGEPKYPVDE SKERDVTYAA PLRVKVRLIN KETGEVKEQE VFMGDFPLMT
     DTGTFIINGA ERVIVSQLVR SPSVYYSEKI DKNGKKGYTA TVIPNRGAWL ELETDAKDIV
     YVRIDRTRKI PVTVLLRALG FGSDQEIIDL LGEDEYLRNT LEKDNTDSAE KALLEIYERL
     RPGEPPTVEN AKSLLDSRFF DPKRYDLANV GRYKVNKKLH IKNRLFNQRL AETLIDPETG
     EVIAEEGSII DRRTLDRILP YLENNVGFRT VRMSGGVVEE DEVRLQSVKI YAPDDQDGEH
     VIRVIGNGLV EKEIKHITPA DIIASINYFF NLLHSVGGTD DIDHLGNRRL RSVGELLQNQ
     FRIGLSRMER VVRERMSIQD PNSITPQALI NIRPVIASIK EFFGSSQLSQ FMDQTNPLAE
     LTHKRRLSAL GPGGLTRERA GFEVRDVHYS HYGRMCPIET PEGPNIGLIN SLSTYAKVNE
     FGFMETPYRR VDPETGKVTA QIDYLTADEE DNYVVAQANM KLAEDGSFID ENIIARFRGE
     NIVVSRDRVD YMDVSPKQVV SAATSCIPFL ENDDSNRALM GANMQRQAVP LLVPEAPIVG
     TGMEHVSAKD SGAAIVSKHR GIVERVTAKE IWVRRLEEVD GKEIKGDLDK YRLQKFIRSN
     QGTSYNQRPI VKEGDVVEKR EILADGPSME KGEMALGRNV LVGFMTWEGY NYEDAIILSE
     RLVKDDVYTS IHIEEYESEA RDTKLGPEEI TRDIPNVGED ALRNLDERGI IRVGAEVKDG
     DILVGKVTPK GVTELTAEER LLHAIFGEKA REVRDTSLRA PHGGDGIVLD VKIFNREDGD
     ELPPGVNQLV RVYIVQKRKI HEGDKMAGRH GNKGVISRIL PEEDMPYLPD GTPIDIMLNP
     LGVPSRMNIG QVLELHLGMA ARRLGLHVAS PVFDGASEED VWATLEEAGM ARDGKTILYD
     GRTGEPFDNR VSVGIMYMIK LAHMVDDKLH ARSTGPYSLV TQQPLGGKAQ FGGQRFGEME
     VWALEAYGAA YTLQEILTVK SDDVVGRVKT YEAIVKGENV PEPGVPESFK VLIKELQSLG
     MDVKMLSSTE EEIEMKELDD EDEQASDKLN LNIDSTESNV