ID   RPOB_ALKMQ              Reviewed;        1246 AA.
AC   A6TWJ0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Amet_4486;
OS   Alkaliphilus metalliredigens (strain QYMF).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Alkaliphilus.
OX   NCBI_TaxID=293826;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=QYMF;
RX   PubMed=27811105; DOI=10.1128/genomea.01226-16;
RA   Hwang C., Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina Del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F.,
RA   Land M.L., Hauser L., Kyrpides N., Mikhailova N., Ye Q., Zhou J.,
RA   Richardson P., Fields M.W.;
RT   "Complete genome sequence of Alkaliphilus metalliredigens strain QYMF, an
RT   alkaliphilic and metal-reducing bacterium isolated from borax-contaminated
RT   leachate ponds.";
RL   Genome Announc. 4:0-0(2016).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000724; ABR50558.1; -; Genomic_DNA.
DR   RefSeq; WP_012065449.1; NC_009633.1.
DR   AlphaFoldDB; A6TWJ0; -.
DR   SMR; A6TWJ0; -.
DR   STRING; 293826.Amet_4486; -.
DR   EnsemblBacteria; ABR50558; ABR50558; Amet_4486.
DR   KEGG; amt:Amet_4486; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001572; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1246
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329161"
FT   REGION          1171..1246
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1171..1189
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1202..1239
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1246 AA;  139729 MW;  461C9FE5A9E0B0E9 CRC64;
     MPHPVQLGKK VRMSYSQINE VLDMPNLIEL QKEAYDWFLD EGLREVFNDI SPIQDYTGNL
     ILEFVDYSLD ENPKYDVGES KERDATYAAP LKVKVRLINK ETGEVKEQEV FMGDFPLMTD
     TGTFIINGAE RVIVSQLVRS PGVYYNRQFD KTGKQLYSAT VIPNRGAWLE YETDSNDVVS
     VRVDRTRKQP ATVLLRALGY GTDQQIKDLL GEDERILATL EKDNTKTAEE GLLEIYKKLR
     PGEPPTIESA TSLINTLFFD AKRYDLAKVG RYKFSKKLSL ANRIMSHKAA ENVSDPQTGE
     ILVEEGTKID REISLLIQNS GINEVHVYSE ENKKIKVIGN HFVDIKTHID FDIQDLKLRE
     RVYYPVLREI LDTFDSEAEI KEALKERKRE LIPKHILKSD IVASINYAFN LAHEIGNVDD
     IDHLGNRRLR SVGELLQNQF RIGLSRMERV VKERMTIQDV DLVTPQALIN IRPVAASIKE
     FFGSSQLSQF MDQTNPLAEL THKRRLSALG PGGLSRERAG FEVRDVHHSH YGRMCPIETP
     EGPNIGLINS LSSYARINEY GFIESPYRKV DKKRDVVTTD IEYLTADEED LFIIAQANEP
     LDEEGKFGNK RVTSRTKFGG IDVVPFDEVD YMDVSPKQVV SVATAMIPFL ENDDANRALM
     GSNMQRQAVP LLITDAPIIG TGMEYKAAKD SGVVVVARNS GIVDYVASNE IVVKLEDGQK
     DRYKLLKFKR SNQGTCINQK PIVSKGERIE AGDVIADGPS TDRGEIALGR NCLVGFMAWE
     GYNFEDAILI NEKLVKEDAL TTIHIEEYES EARDTKLGPE EITRDIPNVG EDSLKDLDER
     GVIRIGAEVE SGDILVGKVT PKGETELTAE ERLLRAIFGE KAREVRDTSL KVPHGENGII
     VDIKVFTREN GDELPPGVNE LVRVYIAKKK KINVGDKMAG RHGNKGVISR ILPQEDMPFL
     EDGTPLEIVL NPLGVPSRMN IGQILEVHLG LAAKALGWKV ATPVFDGANE FDIMDALEES
     GYPRGGKLKL QDGRTGEAFD NPVTVGYMYM LKLHHLVDDK IHARSTGPYS LVTQQPLGGK
     AQFGGQRFGE MEVWALEAYG AAHTLQEILT VKSDDVVGRV KTYECIVKGE NIPEPGVPES
     FKVLIKELQS LCLDVKVLTD DDHELEIKET IDDDAGEMSL EHGDFDYGLE EPTVPEGSHI
     TDEEEKDEDN DSEEALITEE DFEPTSVETE YAEDDDEFDG YNDFKA