RPOB_ALKOO
ID RPOB_ALKOO Reviewed; 1241 AA.
AC A8MLD2;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 76.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Clos_0484;
OS Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain
OS OhILAs)).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Alkaliphilus.
OX NCBI_TaxID=350688;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OhILAs;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E.,
RA Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.;
RT "Complete genome of Alkaliphilus oremlandii OhILAs.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000853; ABW18046.1; -; Genomic_DNA.
DR AlphaFoldDB; A8MLD2; -.
DR SMR; A8MLD2; -.
DR STRING; 350688.Clos_0484; -.
DR PRIDE; A8MLD2; -.
DR EnsemblBacteria; ABW18046; ABW18046; Clos_0484.
DR KEGG; aoe:Clos_0484; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000000269; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1241
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000329162"
FT REGION 1201..1224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1241 AA; 139082 MW; 084A0BCF28EE7F7D CRC64;
MMPHPVQLGK KTRMSYSQID EVLEMPNLID LQKDSYQWFL DEGLKEVFDD VSPIEDYTGN
LILEFVDYSL DENPKYNIED SKERDVTYAA PLKVKVRLIN KTTGEVKEQE VFMGDFPLMT
DTGTFIINGA ERVIVNQLVR SPGVYYSREF DKTGKQLFSA TVIPNRGAWL EYETDSNDIV
SVRIDRTRKQ PVTVLLRALG YGSDAQIKEL LGEDERLLAT LEKDSTKTTD EALLEIYKKL
RPGEPPTVEN AKSLLRTLFF DPKRYDLAKV GRYKFNKKLS LANRILGKKA AANIVDPSTG
EILVEEGTKI DRETSIMVQD SGINEVFIYA EDNKVIKVLG NHFVNINKHI PFNIEELKIS
DKVYYPVLKE ILDTFSQEED IRDALKERAK ELSPKHIIIA DIIASISYEF NLAHDTGNID
DIDHLGNRRS RSVGELLQNQ FRIGLSRMER VVKERMTIQD VDLATPQALI NIRPVAAAIK
EFFGSSQLSQ FMDQNNPLSE LTHKRRLSAL GPGGLSRERA GFEVRDVHNS HYGRMCPIET
PEGPNIGLIN TLSTYARVNE YGFIESPYRK VDKKRGVVTN VIEYLTADEE DLLVIAQANE
PLDEEGRFVN KRIACRMQFG AIDIAPANEV DYMDVSPKQV VSVATAMIPF LENDDANRAL
MGANMQRQAV PLLITDAPII GTGMEYQSAK DSGVVILAKH DGVIDRLSSN EIVIKRDKDG
QKDRYKLLKF KRSNQGTCIN QRPIVSKGER VKAGDVIADG PSTNQGEIAL GRNCLIGFMT
WEGYNYEDAI LINEKLVKED SLTSIHIEEY EAEARDTKLG PEEITRDIPN VGEEALKDLD
ERGIIRIGAE VQSGDILVGK VTPKGETELT AEERLLRAIF GEKAREVRDT SLKVPHGESG
IIVDIKVFSR ENGDELPPGV NELVRAYIAQ KRKINVGDKM AGRHGNKGVI SRVLPAEDMP
FLADGTPLEI VLNPLGVPSR MNIGQVLEVH LGLAAKSLGW NVATPVFDGA NEHDIMDALE
MAGFSRSGKV KLYDGRTGEP FDNDVTVGYM YMLKLHHLVD DKIHARSTGP YSLVTQQPLG
GKAQFGGQRF GEMEVWALEA YGAAHTLQEI LTVKSDDVVG RVKTYECIVK GENIPEPGVP
ESFKVLIKEL QSLCLDVKVL TEEDSEIEIK ESVEDDSGEL SIEYGDLTYE ADGELEIIEP
AEEEQDTDVD YITEDDFESP DPEIDFDEKY LEDDFNSYDD F
