ID   RPOB_ANADF              Reviewed;        1416 AA.
AC   A7HCH7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=Anae109_2221;
OS   Anaeromyxobacter sp. (strain Fw109-5).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter;
OC   unclassified Anaeromyxobacter.
OX   NCBI_TaxID=404589;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fw109-5;
RX   PubMed=25614562; DOI=10.1128/genomea.01449-14;
RA   Hwang C., Copeland A., Lucas S., Lapidus A., Barry K., Glavina Del Rio T.,
RA   Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D.C.,
RA   Detter J.C., Han C.S., Schmutz J., Larimer F.W., Land M.L., Hauser L.J.,
RA   Kyrpides N., Lykidis A., Richardson P., Belieav A., Sanford R.A.,
RA   Loeffler F.E., Fields M.W.;
RT   "Complete genome sequence of Anaeromyxobacter sp. Fw109-5, an anaerobic,
RT   metal-reducing bacterium isolated from a contaminated subsurface
RT   environment.";
RL   Genome Announc. 3:0-0(2015).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000769; ABS26423.1; -; Genomic_DNA.
DR   RefSeq; WP_012097005.1; NC_009675.1.
DR   AlphaFoldDB; A7HCH7; -.
DR   SMR; A7HCH7; -.
DR   STRING; 404589.Anae109_2221; -.
DR   PRIDE; A7HCH7; -.
DR   EnsemblBacteria; ABS26423; ABS26423; Anae109_2221.
DR   KEGG; afw:Anae109_2221; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_7; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000006382; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1416
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000051960"
FT   REGION          1388..1416
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1416 AA;  158370 MW;  7310606D0344715E CRC64;
     MATTIQNNFR IRRNFGKINK IAEIPNLIAI QKSSYDKFLQ ADVPPEKRED TGLQGVFKSV
     FPIKDFNETS SLEFVSYHLE KPKYDVDECH QRGMTYSAPI KVVVRLVVWD KDEETGAQSI
     RDVKEQEVYF GEIPLMTENG TFIINGTERV VVSQLHRSPG AFFDHDKGKS HSSGKLLFNA
     RIIPYRGSWI DFEFDHKDIL YVRIDRRRKL PATVLLRALG GVPDTAKKTP IEFHGSAEEI
     LKYYYDTETI RIEGKGKFEK DLNPDLLKGQ RATRDIRDPK TNEIVVKKNR KFTESAIKKL
     VAAKMKSLPI EPEEVYTKIS AEDVVDEQTG EVLLEVNEEV SEEKLEELRK RGIGEFKVLF
     IDNLNVLPSL RDTLMQDKIS TPEEAIMEIY RRLRPGDPPT PETATNLFVN LFFNAERYDL
     SKVGRLKLNY KFGIEEPLDN TVVTKRDILE VVRFLIDLKN GTPNKDVDDI DHLGNRRVRA
     VGELLENQYR IGLVRMERAI KERMSLQEIE TLMPHDLINA KPVTAVIKEF FGSSQLSQFM
     DQTNPLSEVT HKRRLSALGP GGLTRERAGF EVRDVHSTHY GRICPIETPE GPNIGLIASL
     STYARVNEYG FVETPYRKVE KGRVTDEVSF YSALEEEKHI IAQANAPVDK KGVFTGNIVS
     CRKEGEYINA RPDEVDLMDV SPNQLVSVAA SLVPFLENDD ANRALMGSNM QRQAVPLLRT
     RAPLVGTGIE GIVARDSGVC VVAKRDGVIQ SVDAARIVVK ADVPTSATDV ANEVDIYNLI
     KYQRSNQNTC INQKPIVKPG ERVKKGDVIA DGPATEMGEL ALGQNVVVAF MPWQGYNFED
     SILLSERFLK DDVFTSVHIE EFECVARDTK LGKEEITRDI PNVGEEALKD LDESGIIRIG
     AEVKPGDILV GKITPKGETQ LSPEEKLLRA IFGEKAGDVR DSSLRVPPGV SGTVINAKVF
     SRKGVEKDER AKAIEEMEEA KLLKDQNDEI RIIQDSAFQK IRRLLLGKEV SGRLVDDKGN
     QLLKRGDVLD DALLDTVPQR YWGEIAVEGD VQDLVRKIVE NFEEQKELVK LLFGEKIGRL
     KKGDELPPGV IKMVKVYVAI KRKLAVGDKM AGRHGNKGVV SRVLPEEDLP YLEDGRPVDI
     VLNPLGVPSR MNVGQILETH LGWAAKNVGW RLQEMIEKNF GPDQLRKKLK KAFEGVEGSD
     RLGQLIDELP AQELPKLATK LKEGMHVATP VFDGAHEDEM KALLGEGSAT MQSILFDGRT
     GEPFDQDVTV GVMYMLKLHH LVDEKIHARS IGPYSLVTQQ PLGGKAQFGG QRLGEMEVWA
     MEAYGAAYSL QEFLTVKSDD VVGRTRMYEA IVKGENTLES GLPESFNVLI KELQSLALDV
     ELLETPEAKA AREQAEGELG GPLGTPRGAA AEKNTA