RPOB_ANAMF
ID RPOB_ANAMF Reviewed; 1382 AA.
AC Q8KWX4; B9KHW1;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=AMF_194;
OS Anaplasma marginale (strain Florida).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Anaplasmataceae; Anaplasma.
OX NCBI_TaxID=320483;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12710612; DOI=10.1099/ijs.0.02411-0;
RA Taillardat-Bisch A.V., Raoult D., Drancourt M.;
RT "RNA polymerase beta-subunit-based phylogeny of Ehrlichia spp., Anaplasma
RT spp., Neorickettsia spp. and Wolbachia pipientis.";
RL Int. J. Syst. Evol. Microbiol. 53:455-458(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Florida;
RX PubMed=19134224; DOI=10.1186/1471-2164-10-16;
RA Dark M.J., Herndon D.R., Kappmeyer L.S., Gonzales M.P., Nordeen E.,
RA Palmer G.H., Knowles D.P. Jr., Brayton K.A.;
RT "Conservation in the face of diversity: multistrain analysis of an
RT intracellular bacterium.";
RL BMC Genomics 10:16-16(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM73633.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACM49073.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF389472; AAM73633.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP001079; ACM49073.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_010267184.1; NC_012026.1.
DR AlphaFoldDB; Q8KWX4; -.
DR SMR; Q8KWX4; -.
DR STRING; 320483.AMF_194; -.
DR EnsemblBacteria; ACM49073; ACM49073; AMF_194.
DR GeneID; 7398648; -.
DR KEGG; amf:AMF_194; -.
DR PATRIC; fig|320483.3.peg.221; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_5; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000007307; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1382
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047852"
FT CONFLICT 6..7
FT /note="GS -> D (in Ref. 1; AAM73633)"
FT /evidence="ECO:0000305"
FT CONFLICT 18..24
FT /note="DAVPRLS -> VFFVGSL (in Ref. 1; AAM73633)"
FT /evidence="ECO:0000305"
FT CONFLICT 29..34
FT /note="IDIRDS -> YNSRF (in Ref. 1; AAM73633)"
FT /evidence="ECO:0000305"
FT CONFLICT 76
FT /note="R -> K (in Ref. 1; AAM73633)"
FT /evidence="ECO:0000305"
FT CONFLICT 807
FT /note="V -> L (in Ref. 1; AAM73633)"
FT /evidence="ECO:0000305"
FT CONFLICT 869..884
FT /note="PLGPEKIMRSVPDVNE -> LRSREDNAVCLMY (in Ref. 1;
FT AAM73633)"
FT /evidence="ECO:0000305"
FT CONFLICT 894..914
FT /note="GIVNIGAEVSASSILVGKVTP -> DRDIVPRYLQVVSGGQGYA (in
FT Ref. 1; AAM73633)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1382 AA; 154508 MW; A66AFAD1E3DC619D CRC64;
MSSAGGSSGP GYVLNDFDAV PRLSYAKSID IRDSLTDLIR IQRDSYDAFI GIDRDGSSGI
QSIFEAMFPI RDLLGRAVLE FVGYNIGEPQ YDEYECIKRG ITFSVPIRIT LRFVVWKVQE
VSFKEVKYVV DEDTSERSVK YMKEQEVSIG DLPMMTSYGT FIINGIERVI VSQMHRSPGV
FFDSDKGKTY SSGKLIYSAR VIPYRGSWLD FEFDIKDIIY FRIDKKRKLP VSYLLKALGM
SNNDILDAFY DKVIYTRCDK GWRVPFIVDR FKGVRLSYDL MDVDGSVLVK ANTRITLRIA
KKLYADGLRE YMVPFSGITG MFVAADLVDP SSGAVIVSAG ETITSEHIVK LELFDISEIA
FLNIDFLTVG PYVLNTLFLD KNMTQEDALF EIYRVLRSGE SPNLDAVKSF FKGLFFESER
YDLSVVGRIK LNSHLGLDVD ENTTVLTKED IVQVVKKLVL LRDGEGVVDD IDHLGNRRVR
SVGEFIENQF RIGILRLERM IMDYMSSVNF DNAMPCDFVN PKVLATVLKD FFSSSQLSQF
MDQTNPLSEV THKRRLSALG PGGLTRERAG FEVRDVHPTH YGRICPIETP EGQNIGLISS
LAIYAKINKY GFIESPYRKV ENRVVTDKVE YLLAMQEGDY YIADAGAAID ENNRFVDDML
YCRHGGNFVM VKSEDVDYVD VSPKQIVSVA ASLIPFLENN DANRALMGSN MQRQAVPLLK
SEAPLVGTGM EFVVAAGSGA VVLAKRDGIV HRVDGSYIVI RAFDANKDEC LGVDIYRLRK
FQRSNHNTCI NQRPVVKLGD YVKANDVIAD GSAIDRGELA LGKNVLVAFM SWQGYNFEDS
IVISSDVVKR DVFTSIHIEE FECVVRDTPL GPEKIMRSVP DVNEESLSHL DDVGIVNIGA
EVSASSILVG KVTPRPPVSL PPETKLLVTI FGEKVFDCVD SSLYLPPDVE GTVIDVHVFV
RRGVEENDRS LLIKQSEISS FVKERDYEID VVSEYFHDEL RKLLRNAGVK VKGYSDLDSF
FAEASDDTLW STGLADAKVA AKVKDMRERF DSIVGEAHRK FEQKVDKLNY GYDLPQGVLT
IVKVFVAVKH NLQPGDKMAG RHGNKGVISR IVPAEDMPHL EDGTPVDIIL NSLGVPSRMN
IGQILETHLG WAAVNLGKKI GRILDKGGPS MIADLRDFLD KIYDGQKLKS DIASMSSEAL
LVFANRLRKG VPMAAPVFEG PKDAQISKLL ELAEVDPSGQ VYLYDGRLGK KFDRKITVGY
IYMLKLHHLV DDKIHARSVG PYGLVTQQPL GGKSHFGGQR FGEMECWALQ AYGAAYTLQE
MLTVKSDDIV GRVKIYESII KGDSNFECGI PESFNVMVKE LRSLCLNVVL KQDKEFTSGE
VE
