RPOB_AROAE
ID RPOB_AROAE Reviewed; 1377 AA.
AC Q5P339;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=AZOSEA21500;
GN ORFNames=ebA3818;
OS Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC Rhodocyclaceae; Aromatoleum.
OX NCBI_TaxID=76114;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EbN1;
RX PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA Reinhardt R.;
RT "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT bacterium, strain EbN1.";
RL Arch. Microbiol. 183:27-36(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CR555306; CAI08275.1; -; Genomic_DNA.
DR RefSeq; WP_011237966.1; NC_006513.1.
DR AlphaFoldDB; Q5P339; -.
DR SMR; Q5P339; -.
DR STRING; 76114.ebA3818; -.
DR EnsemblBacteria; CAI08275; CAI08275; ebA3818.
DR KEGG; eba:ebA3818; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000006552; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1377
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224024"
SQ SEQUENCE 1377 AA; 152839 MW; 14331EC8A655DE9F CRC64;
MAYSYTEKKR IRKSFAKRAA VLNVPFLLAT QIESFASFLQ ADTPPPSRLN HGLQAAFTSI
FPISSHSGNA RLEFVQYMLG EPAFDVKECQ QRGLTFASPL RARVRLVILD RDAPKETVKE
VKEQEVYMGE IPLMTTTGSF VINGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDALYF RVDRRRKMPA TILLRAIGMG PEEILATFHD FDAFHLRGNE
AAFELVPDRL RGDVAKFDIT DGAGNVIVAR DKRITAKHIR ELDQAGVKMI SVPDEFLLGR
IIARNIVDAE TGEIVARAND EITEDLLDKL RDAGVKDLET LYVNDLDRGA YISQTLRIDE
TADQWAARVA IYRMMRPGEP PTEDAVEALF QGLFYSEERY DLSSVGRMKF NRRAYPEKIE
DKAPSWLKRF YETVGPRGEE GPATLSNEDI LAVIGVLVEL RNGRGEIDDI DHLGNRRVRS
VGELAENQFR AGLVRVERAV KERLSQAESD NLMPHDLINA KPISAAIKEF FGSSQLSQFM
DQTNPLSEIT HKRRVSALGP GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL
AVYAQTNRHG FLETPYRKVV DSKVTDQIDF LSAIEEGQYV IAQANAEIDA DGMLEGDLVS
CRHKGEFALS TADQVQYMDV APGQIVSVAA SLIPFLEHDD ANRALMGANM QRQAVPCLRP
EKPLVGTGIE RTVAVDSGTA VQAMRGGIVD YVDANRIVVR VNDDETLAGE VGVDIYNMIK
YTRSNQNTNI NQRPVVKVGD HIGKGDVIAD GASTDLGELA LGQNMLVAFM PWNGYNFEDS
ILISERVVAE DRFTSIHIEE LTVVARDTKL GPEEITRDIA SLGEAQLSRL DESGIVYIGA
EVDAGDVLVG KVTPKGETQL TPEEKLLRAI FGEKASDVKD TSLRVSSGMN GTVIDVQVFT
REGIERDKRA QSIIDDMLRS FKTDLADQMR IVERDAFARI RRLIVGQKAN GGPKKLLKGT
AITGEYLDSL EFYHWFDIRM ADEELAAQLE AIREGLEKTR KDFDQAFEIK KKKLTQGDEL
PPGVQKMVKV YLAVKRRLQP GDKMAGRHGN KGVVSRIVPI EDMPHMADGT PVDIVLNPLG
VPSRMNIGQI LETHLGWASK ALGNKIGTML RANAAAAEVR ELLEHIYNDN GRPEDMGSLK
DDEVIELASN LSKGVPFATP VFDGAKEEEI EAMFELAGLQ PGGQVTLYDG RTGDAFDRQV
TVGYKHVLKL HHLVDDKMHA RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY
TLQEMLTVKS DDVTGRTKVY ESIVKGEHKI DAGMPESFNV LVKEIRSLAI DIDLDTY
