RPOB_AYWBP
ID RPOB_AYWBP Reviewed; 1273 AA.
AC Q2NJ15;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=AYWB_461;
OS Aster yellows witches'-broom phytoplasma (strain AYWB).
OC Bacteria; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae;
OC Candidatus Phytoplasma; Candidatus Phytoplasma asteris.
OX NCBI_TaxID=322098;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AYWB;
RX PubMed=16672622; DOI=10.1128/jb.188.10.3682-3696.2006;
RA Bai X., Zhang J., Ewing A., Miller S.A., Jancso Radek A., Shevchenko D.V.,
RA Tsukerman K., Walunas T., Lapidus A., Campbell J.W., Hogenhout S.A.;
RT "Living with genome instability: the adaptation of phytoplasmas to diverse
RT environments of their insect and plant hosts.";
RL J. Bacteriol. 188:3682-3696(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000061; ABC65578.1; -; Genomic_DNA.
DR RefSeq; WP_011412742.1; NC_007716.1.
DR AlphaFoldDB; Q2NJ15; -.
DR SMR; Q2NJ15; -.
DR STRING; 322098.AYWB_461; -.
DR PRIDE; Q2NJ15; -.
DR EnsemblBacteria; ABC65578; ABC65578; AYWB_461.
DR KEGG; ayw:AYWB_461; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_14; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR PhylomeDB; Q2NJ15; -.
DR BRENDA; 2.7.7.6; 14809.
DR Proteomes; UP000001934; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1273
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000237297"
SQ SEQUENCE 1273 AA; 143615 MW; 10B25904389979B7 CRC64;
MGYHNVKYGK KAQRRNYSKT IYDVDLPNLI EIQNQSFDWF LKHGIKELLQ DFCPIESYNG
DLKIHFDDYF LTPPKYSIEE TKIKDISYVA QLFVKTTLEN VLTGETKQSN ILLTELPLMT
STGTFIINGT ERVVVSQIVR SASVYFAGNF DIKLNRTIYS GQVIPSRGAW IEYEEGSKEI
LYAKLDRSKK IPLSNFIYAL GFDSKEAIEN VFGKSAILDS VFDKETDMDS DNALVELYSK
IRQGEKVPVD TARDFIRTRL FDQKKYDLAK VGRYKFNKKL DVLTRAENTY LACDFVNPEN
QEIMIAQDEL LTKEKIAILK QNRHFLLQEI FDAKHNLENE TDEEILAYKK YPQKNELFTK
TNIINSRTGE VLVAKDTLVN DDIINHLRHN IHTLDEKVSK FFLGTKDIYQ KEADRQGVFN
EILEVYTSKD ESGKLYNKVK LIGNDQRETK KHITLSDVIA SINYYLNLYE NVGTVDDIDH
LGNRRLRLIG ELLKNQFRIG LTRAEKNIKD MISTSKFGEV NGPGDLVNFT FLMSVIKTFF
TNSRLSQFMD QINPLAELTQ KRRVSALGIG GINRDRAGIE VRDVHNSHYG RLCPIETPEG
PSIGLITSLS TYAKVNKYGF IQTPFFKVLQ QDGKTTLSRD IDYLTADQEK EEIIASAGFV
LDANNSFQDK KIIARSNGET GIFERNQITY ADVSPKQIVS VATSSIPFLE HNDASRALMG
ANMQRQAVPL LIPESPIVGT GVEYRAAKDS GCLIIARESG FVTYVDAQKI IITKKPNQTV
SLNGKTLYDT TQEFTYTQAK VLYENNHKEY QAEYTLINFA KSNQDTLVLQ KPIVVLGEHI
NEGDILVSGP STSQGELALG RNVTVAFMTW EGYNYEDAII MSEELVKHDV YTSIHIDKYE
VQTRELKKGS GQEEITREVP NVGADAIKNL DERGIIIPGS EVKEGDILVG KITPQGNIEP
SPSEKLIQIV IGEKAREYKD SSLRVPFGEG GIVQSVHYFS RKNGDVLPAG VNENIRVFIA
KKRKINEGDK MAGRHGNKGV ISRILPKEDL PYMADGTPID IMLNPLGVPS RMNIGQILEI
HLGMAAKKLG IKVATPVFDG VNDYDLKEIM KEANLEPDGK MVLYDGRTGE PYDSRISVGV
MYMVKLSHMV DDKLHARNVG PYTLVTQQPM GGKVQNGGQR FGEMEVWALY AYGAAHTLQE
ILTVKSDDIV GRNKTYSAIV QGTQLPKPSI PESFRVFIKE LQSLGLYVEL IKTDTKENEV
NKSLIDYKKE GYN
