RPOB_AZOSB
ID RPOB_AZOSB Reviewed; 1377 AA.
AC A1KB34;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=azo3424;
OS Azoarcus sp. (strain BH72).
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Azoarcus.
OX NCBI_TaxID=418699;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BH72;
RX PubMed=17057704; DOI=10.1038/nbt1243;
RA Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT strain BH72.";
RL Nat. Biotechnol. 24:1385-1391(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AM406670; CAL96040.1; -; Genomic_DNA.
DR RefSeq; WP_011767147.1; NC_008702.1.
DR AlphaFoldDB; A1KB34; -.
DR SMR; A1KB34; -.
DR STRING; 62928.azo3424; -.
DR PRIDE; A1KB34; -.
DR EnsemblBacteria; CAL96040; CAL96040; azo3424.
DR KEGG; azo:azo3424; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_4; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002588; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1377
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300279"
SQ SEQUENCE 1377 AA; 152990 MW; C98A90812338D5E2 CRC64;
MAYSYTEKKR IRKSFAKRAA VLDAPFLLAT QIESFASFLQ AETPPESRYN QGLQAAFTSI
FPISSHSGNA RLEFVQYMLG EPAFDVKECQ QRGLTFASPL RARVRLVIMD REAPKETIKE
VKEQEVYMGE IPLMTTTGSF VINGTERVIV SQLHRSPGVF FEHDRGKTHS SGKLLFSARI
IPYRGSWLDF EFDPKDYLYF RVDRRRKMPV TILLRAIGMT PEEILETFHD FDAFHLSAEG
AQFELVPDRL RGEVARFDIV DPTGKVIVAR DKRITAKHIR ELDQAGIKMI GVPDDFVLGR
IIARNVVDPE TGELLARANE EITEDLLGKL RDAGVRDLVT LYVNDLDRGA YISSTLRIDE
TADQWAARVA IYRMMRPGEP PTEEAVEALF QGLFYAPERY DLSTVGRMKF NRRAYPEKID
DKAPGWLKRF YDKVGPHGEE GEGVLANEDI LAVIGVLVEL RNGRGEIDDI DHLGNRRVRS
VGELAENQFR AGLVRVERAV KERLSQAESD NLMPHDLINA KPISAAIKEF FGSSQLSQFM
DQTNPLSEIT HKRRVSALGP GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL
AVYARTNRHG FLETPYRKVT DGKVTDQIDF LSAIEEGQYV IAQANAEIGG DGALEGDLVS
CRHKGEFMLA TADQVQYMDV APGQIVSVAA SLIPFLEHDD ANRALMGANM QRQAVPCLRP
EKPLVGTGIE RTVAVDSGTA VQAMRGGVVD YVDAQRVVVR VNDNEALAGE VGVDIYNMIK
YTRSNQNTNI NQRPVVRVGD LIAKGDVIAD GASTDLGELA LGQNMLVAFM PWNGYNFEDS
ILISERVVAD DRFTSIHVEE LTVVARDTKL GPEEITRDIA SLGEAQLSRL DESGIVYIGA
EVEAGDVLVG KVTPKGETQL TPEEKLLRAI FGEKASDVKD TSLRVPSGMT GTVIDVQVFT
REGIERDKRA QSIIDDQLRS FKTDLADQMR IVERDAFARI QRMIVGQKAN GGPKKLAKGA
AITAEYLETL EFYHWFDIRM ADEELAIQLE AVREGLEKTR KDFEQAFEIK KKKLTQGDEL
PPDVQKMVKV YLAVKRRLQP GDKMAGRHGN KGVVSKIVPV EDMPYMEDGT PVDIVLNPLG
VPSRMNIGQI LETHLGWAAK GLGQKIDRLL RANAAAGEVR GLLEEIYNGS GKPEDFSEFT
DGDVIELATN LKKGVPFATP VFDGAKEEEI AKMLELAGLP VGGQVTLFDG RTGEAFERKV
TVGYKHVLKL HHLVDDKMHA RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV WALEAYGAAY
TLQEMLTVKS DDVTGRTKVY ENIVKGEHKI DSGMPESFNV LVKEIRSLAI DIDLDRE
