ID   RPOB_AZOVD              Reviewed;        1358 AA.
AC   C1DKK5;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Avin_06170;
OS   Azotobacter vinelandii (strain DJ / ATCC BAA-1303).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=322710;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DJ / ATCC BAA-1303;
RX   PubMed=19429624; DOI=10.1128/jb.00504-09;
RA   Setubal J.C., Dos Santos P., Goldman B.S., Ertesvaag H., Espin G.,
RA   Rubio L.M., Valla S., Almeida N.F., Balasubramanian D., Cromes L.,
RA   Curatti L., Du Z., Godsy E., Goodner B., Hellner-Burris K., Hernandez J.A.,
RA   Houmiel K., Imperial J., Kennedy C., Larson T.J., Latreille P., Ligon L.S.,
RA   Lu J., Maerk M., Miller N.M., Norton S., O'Carroll I.P., Paulsen I.,
RA   Raulfs E.C., Roemer R., Rosser J., Segura D., Slater S., Stricklin S.L.,
RA   Studholme D.J., Sun J., Viana C.J., Wallin E., Wang B., Wheeler C., Zhu H.,
RA   Dean D.R., Dixon R., Wood D.;
RT   "Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized
RT   to support diverse anaerobic metabolic processes.";
RL   J. Bacteriol. 191:4534-4545(2009).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001157; ACO76868.1; -; Genomic_DNA.
DR   RefSeq; WP_012699296.1; NC_012560.1.
DR   AlphaFoldDB; C1DKK5; -.
DR   SMR; C1DKK5; -.
DR   STRING; 322710.Avin_06170; -.
DR   EnsemblBacteria; ACO76868; ACO76868; Avin_06170.
DR   KEGG; avn:Avin_06170; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000002424; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1358
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000214470"
SQ   SEQUENCE   1358 AA;  150932 MW;  C0B36D4C870C4F1C CRC64;
     MAYSYTEKKR IRKDFSKLPD VMDVPYLLAI QLDSYREFLQ AGVSKDKLRD VGLHAAFKSV
     FPIISYSGNA ALEYVGYRLG EPAFDVKECV LRGVTFAVPL RVKVRLIIFD KESSNKAIKD
     IKEQEVYMGE IPLMTENGTF IVNGTERVIV SQLHRSPGVF FDHDRGKTHS SGKLLYSARI
     IPYRGSWLDF EFDPKDAVFV RIDRRRKLPA SVLLRALGYS TEEVLDIFYD TNVFHVTEQG
     LSLELVPQRL RGEIAVFDIK DASGKVIVEQ GRRITARHIN QLEKAGIKEL DVPLEYVLGR
     TSAKVIVHPS TGEIIAECNS ELTQDLLVKI AKAQVVRIET LYTNDIDCGP FISDTLKIDS
     TTNQLEALVE IYRMMRPGEP PTKDAAETLF NNLFFSAERY DLSAVGRMKF NRRIGREEIE
     GAGVLSREDI VDVLKTLVDI RNGKGIVDDI DHLGNRRVRC VGEMAENQFR VGLVRVERAV
     KERLSMAESE GLMPQDLINA KPVAAAVKEF FGSSQLSQFM DQNNPLSEIT HKRRVSALGP
     GGLTRERAGF EVRDVHPTHY GRVCPIETPE GPNIGLINSL AAYARTNQYG FLESPYRVVK
     EGQVTDEIVF LSAIEEADHV IAQASATLDE NGRLIDELVA VRHLNEFTVK APEDVTLMDV
     SPRQVVSVAA SLIPFLEHDD ANRALMGSNM QRQAVPTLRA DKPLVGTGME RNVARDSGVC
     VVARRGGVID SVDASRIVVR VNNDEVETGE AGVDIYNLTK YTRSNQNTCI NQRPLVSKGD
     QVARGDIMAD GPSTDMGELA LGQNMRVAFM PWNGFNFEDS ICLSERVVQE DRFTTIHIQE
     LTCVARDTKL GPEEITADIP NVGEAALNKL DEAGIVYVGA EVMAGDILVG KVTPKGETQL
     TPEEKLLRAI FGEKASDVKD TSLRVPTGTK GTVIDVQVFT RDGVERDSRA LAIERMQLDE
     IRKDLNEEFR IVEGATFERL RAALVGAVCE GGAGLKKGTE IDDGILDGLE RGQWFKLRMA
     DDALNEQLEK AQAYLADRRQ LLDDKFEDKK RKLQQGDDLA PGVLKIVKVY LAIKRRIQPG
     DKMAGRHGNK GVVSVIMPVE DMPYDANGTP VDIVLNPLGV PSRMNVGQIL ETHLGLAAKG
     LGEKINRMLE EQRKVAELRQ FLGEVYNEIG GRQKEDLDSL SDSEILDLAS NLRGGVPMAT
     PVFDGARETE IKAMLKLADL PESGQMRLFD GRTGNQFERP TTVGYMYMLK LNHLVDDKMH
     ARSTGSYSLV TQQPLGGKAQ FGGQRFGEME VWALEAYGAA YTLQEMLTVK SDDVNGRTKM
     YKNIVDGDHR MEAGMPESFN VLIKEIRSLG IDIELETE