ID   RPOB_BACCR              Reviewed;        1177 AA.
AC   Q81J48;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BC_0122;
OS   Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC
OS   15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=226900;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373
RC   / NCTC 2599 / NRRL B-3711;
RX   PubMed=12721630; DOI=10.1038/nature01582;
RA   Ivanova N., Sorokin A., Anderson I., Galleron N., Candelon B., Kapatral V.,
RA   Bhattacharyya A., Reznik G., Mikhailova N., Lapidus A., Chu L., Mazur M.,
RA   Goltsman E., Larsen N., D'Souza M., Walunas T., Grechkin Y., Pusch G.,
RA   Haselkorn R., Fonstein M., Ehrlich S.D., Overbeek R., Kyrpides N.C.;
RT   "Genome sequence of Bacillus cereus and comparative analysis with Bacillus
RT   anthracis.";
RL   Nature 423:87-91(2003).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE016877; AAP07204.1; -; Genomic_DNA.
DR   RefSeq; NP_830003.1; NC_004722.1.
DR   RefSeq; WP_000147553.1; NZ_CP034551.1.
DR   AlphaFoldDB; Q81J48; -.
DR   SMR; Q81J48; -.
DR   STRING; 226900.BC_0122; -.
DR   MetOSite; Q81J48; -.
DR   PRIDE; Q81J48; -.
DR   EnsemblBacteria; AAP07204; AAP07204; BC_0122.
DR   GeneID; 67504809; -.
DR   KEGG; bce:BC0122; -.
DR   PATRIC; fig|226900.8.peg.124; -.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000001417; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1177
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047858"
FT   REGION          1147..1177
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1158..1177
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1177 AA;  131865 MW;  E9F2CE6C14F2BFB9 CRC64;
     MTGQLVQYGR HRQRRSYARI SEVLELPNLI EIQTSSYQWF LDEGLREMFQ DISPIEDFTG
     NLSLEFIDYS LGEPKYSVDE CKERDVTYAA PLRVKVRLIN KETGEVKEQD VFMGDFPLMT
     ETGTFVINGA ERVIVSQLVR SPSVYYSGKV DKNGKRGFTA TVIPNRGAWL EYETDAKDVV
     YVRIDRTRKL PVTVLLRALG FGSDQEITEL LGDNEYLSNT LEKDNTDSTE KALLEIYERL
     RPGEPPTVEN AKSLLVSRFF DPKRYDLANV GRYKINKKLH IKNRLFNQRL AETLVDPETG
     EILAAEGTIL DRRTLDRILP YLEKNIGFKT AKPMGGVVEG DVELQSIKIY APESEGERVI
     NVIGNANITR DVKHITPGDI LASISYFFNL LYKVGDTDDI DHLGNRRLRS VGELLQNQFR
     IGLSRMERVV RERMSIQDTN AITPQALINI RPVIASIKEF FGSSQLSQFM DQTNPLAELT
     HKRRLSALGP GGLTRERAGF EVRDVHYSHY GRMCPIETPE GPNIGLINSL SSFAKVNEFG
     FIETPYRRVD PETGLVTGHV DYLTADEEDN YVVAQANMKL SDEGEFLSED IVARFRGENI
     VTNRERIDYM DVSPKQVVSA ATACIPFLEN DDSNRALMGA NMQRQAVPLM NPESPIVGTG
     MEYVSAKDSG AAVICKHPGV VERVEAREVW VRRYVEVDGQ TVKGDLDRYK MQKFIRSNQG
     TCYNQRPIVS VGNEVVKGEI LADGPSMELG ELALGRNVLV GFMTWDGYNY EDAIIMSERL
     VKDDVYTSIH IEEYESEARD TKLGPEEITR DIPNVGEDAL RNLDERGIIR VGAEVKDGDL
     LVGKVTPKGV TELTAEERLL HAIFGEKARE VRDTSLRVPH GGGGIILDVK VFNREDGDEL
     PPGVNQLVRA YIVQKRKISE GDKMAGRHGN KGVISRILPE EDMPYLPDGT PIDIMLNPLG
     VPSRMNIGQV LELHLGMAAR YLGIHIATPV FDGAREEDVW GTIEEAGMAN DAKTILYDGR
     TGEPFDNRVS VGVMYMIKLA HMVDDKLHAR STGPYSLVTQ QPLGGKAQFG GQRFGEMEVW
     ALEAYGAAYT LQEILTVKSD DVVGRVKTYE AIVKGENVPE PGVPESFKVL IKELQSLGMD
     VKMMSSDDTE IEMRDTEDDD DHQSADKLNV EVETTKE