ID   RPOB_BACFN              Reviewed;        1270 AA.
AC   Q5L897;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BF4015;
OS   Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019
OS   / NCTC 9343 / Onslow).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=272559;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow;
RX   PubMed=15746427; DOI=10.1126/science.1107008;
RA   Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V.,
RA   Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A.,
RA   Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A.,
RA   Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J.,
RA   Barrell B.G., Parkhill J.;
RT   "Extensive DNA inversions in the B. fragilis genome control variable gene
RT   expression.";
RL   Science 307:1463-1465(2005).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CR626927; CAH09691.1; -; Genomic_DNA.
DR   RefSeq; WP_005791521.1; NC_003228.3.
DR   AlphaFoldDB; Q5L897; -.
DR   SMR; Q5L897; -.
DR   STRING; 272559.BF9343_3910; -.
DR   EnsemblBacteria; CAH09691; CAH09691; BF9343_3910.
DR   GeneID; 66331185; -.
DR   KEGG; bfs:BF9343_3910; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_10; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000006731; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 3.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1270
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000224031"
SQ   SEQUENCE   1270 AA;  142454 MW;  3B625FF19595460D CRC64;
     MSSNTVNQRV NFASTKNPLE YPDFLEVQLK SFQDFLQLDT PPEKRKKEGL YKVFAENFPI
     ADTRNNFVLE FLDYYIDPPR YTIDDCIERG LTYSVPLKAK LKLYCTDPDH EDFDTVIQDV
     FLGPIPYMTD KATFVINGAE RVVVSQLHRS PGVFFGQSVH ANGTKLYSAR IIPFKGSWIE
     FATDINNVMY AYIDRKKKLP VTTLLRAIGF ENDKDILEIF NLAEDVKVNK TNLKKVVGRK
     LAARVLKTWI EDFVDEDTGE VVSIERNEVI IDRETVIEPE HIDEIIDSGV QNILIHKEEP
     NQSDYSIIYN TLQKDPSNSE KEAVLYIYRQ LRNADPADDA SAREVINNLF FSEKRYDLGD
     VGRYRINKKL NLTTDMDVRV LTKEDIIEII KYLIELINSK ADVDDIDHLS NRRVRTVGEQ
     LSNQFAVGLA RMSRTIRERM NVRDNEVFTP IDLINAKTIS SVINSFFGTN ALSQFMDQTN
     PLAEITHKRR MSALGPGGLS RERAGFEVRD VHYTHYGRLC PIETPEGPNI GLISSLCVFA
     KINDLGFIET PYRKVDNGKV DLSENGLVYL TAEEEEAKII AQGNAPLNDD GTFIRNKVKS
     RQDADYPVVE PSEVELMDVA PQQIASIAAS LIPFLEHDDA NRALMGSNMM RQAVPLLRSE
     APIVGTGIER QLVRDSRTQI AAEGDGVIDF VDATTIRILY DRTEDEEFVS FEPALKEYRI
     PKFRKTNQNM TIDLRPTCNK GDRVTKGQIL TEGYSTENGE LALGKNLLVA YMPWKGYNYE
     DAIVLNERVV REDLLTSVHV EEYSLEVRET KRGMEELTSD IPNVSEEATK DLDENGIVRV
     GARIQPGDIL IGKITPKGES DPSPEEKLLR AIFGDKAGDV KDASLKASPS LKGVIIDKKL
     FSRVIKNRSS KLADKALLPK IDDEFESKVA DLKRILVKKL MVLTEGKVSQ GVKDYLGAEV
     IAKGSKFSAS DFDSLDFTAI QLSDWTNDDH ANGMIRDLIL NFIKKYKELD AELKRKKFAI
     TIGDELPAGI IQMAKVYIAK KRKIGVGDKM AGRHGNKGIV SRVVRQEDMP FLEDGTPVDI
     VLNPLGVPSR MNIGQIFEAV LGRAGKNLGV KFATPIFDGA TLDDLNEWTD KAGLPRYCKT
     YLCDGGTGER FDQPATVGVT YMLKLGHMVE DKMHARSIGP YSLITQQPLG GKAQFGGQRF
     GEMEVWALEG FGASHILQEI LTIKSDDVVG RSKAYEAIVK GEPMPQPGIP ESLNVLLHEL
     RGLGLSINLE