RPOB_BACLD
ID RPOB_BACLD Reviewed; 1193 AA.
AC Q65PB5; Q62ZQ4;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN OrderedLocusNames=BLi00125, BL02798;
OS Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 /
OS NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=279010;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX PubMed=15383718; DOI=10.1159/000079829;
RA Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P.,
RA Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.;
RT "The complete genome sequence of Bacillus licheniformis DSM13, an organism
RT with great industrial potential.";
RL J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B.,
RA Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A.,
RA Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M.;
RT "Complete genome sequence of the industrial bacterium Bacillus
RT licheniformis and comparisons with closely related Bacillus species.";
RL Genome Biol. 5:R77.1-R77.12(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AE017333; AAU39099.1; -; Genomic_DNA.
DR EMBL; CP000002; AAU21754.1; -; Genomic_DNA.
DR RefSeq; WP_011197480.1; NC_006322.1.
DR AlphaFoldDB; Q65PB5; -.
DR SMR; Q65PB5; -.
DR STRING; 279010.BL02798; -.
DR PRIDE; Q65PB5; -.
DR EnsemblBacteria; AAU21754; AAU21754; BL02798.
DR KEGG; bld:BLi00125; -.
DR KEGG; bli:BL02798; -.
DR PATRIC; fig|279010.13.peg.115; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR BioCyc; BLIC279010:BLI_RS00630-MON; -.
DR Proteomes; UP000000606; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1193
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224028"
FT REGION 1153..1193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1193 AA; 133692 MW; 843CECE45C505D5F CRC64;
MTGQLVQYGR HRQRRSYARI SEVLELPNLI EIQTSSYQWF LDEGLREMFQ DISPIEDFTG
NLSLEFIDYS LGEPKYPVEE SKERDVTYSA PLRVKVRLIN KETGEVKDQD VFMGDFPIMT
DTGTFIINGA ERVIVSQLVR SPSVYFSGKV DKNGKKGFTA TVIPNRGAWL EYETDAKDVV
YVRIDRTRKL PVTVLLRALG FGSDQEIIDL IGENEYLRNT LDKDNTENTD KALLEIYERL
RPGEPPTVEN AKSLLDSRFF DPKRYDLASV GRYKINKKLH IKNRLFNQRL AETLVDPETG
EILAEKGAIL DRRTLDKVLP YLENGIGFKK LYPNGGVVED EVTLQSIKIY APTDQEGEQT
INVIGNAYIE EGVKNITPSD IIASISYFFN LLHGVGDTDD IDHLGNRRLR SVGELLQNQF
RIGLSRMERV VRERMSIQDT NTITPQQLIN IRPVIASIKE FFGSSQLSQF MDQTNPLAEL
THKRRLSALG PGGLTRERAG MEVRDVHYSH YGRMCPIETP EGPNIGLINS LSSFAKVNRF
GFIETPYRRV DPETGKVTPR IDYLTADEED NYVVAQANAR LNDDGSFVDD SIVARFRGEN
TVVPKDRVDY MDVSPKQVVS AATACIPFLE NDDSNRALMG ANMQRQAVPL MQPESPIVGT
GMEYVSAKDS GAAVICRHPG IVERVEAKNI WVRRYEEVDG QKVKGNLDKY SLLKFVRSNQ
GTCYNQRPIV SVGDEVEKGE ILADGPSMEK GELALGRNVM VGFMTWDGYN YEDAIIMSER
LVKDDVYTSI HIEEYESEAR DTKLGPEEIT RDIPNVGEDA LRNLDERGII RVGAEVKDGD
LLVGKVTPKG VTELTAEERL LHAIFGEKAR EVRDTSLRVP HGGGGIILDV KVFNREDGDE
LPPGVNQLVR VYIVQKRKIS EGDKMAGRHG NKGVISKILP EEDMPYLPDG TPIDIMLNPL
GVPSRMNIGQ VLELHLGMAA RRLGLHVASP VFDGAREEDV WETLEEAGMS RDAKTVLYDG
RTGEPFDNRV SVGIMYMIKL AHMVDDKLHA RSTGPYSLVT QQPLGGKAQF GGQRFGEMEV
WALEAYGAAY TLQEILTVKS DDVVGRVKTY EAIVKGDNVP EPGVPESFKV LIKELQSLGM
DVKILSSDEE EIEMRDLEDD EDAKQNEGLS LPNDEESEEL VSADAERDVV TKE
