RPOB_BACMK
ID RPOB_BACMK Reviewed; 1177 AA.
AC A9VP69;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN OrderedLocusNames=BcerKBAB4_0097;
OS Bacillus mycoides (strain KBAB4) (Bacillus weihenstephanensis).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=315730;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KBAB4;
RX PubMed=17434157; DOI=10.1016/j.cbi.2007.03.003;
RA Lapidus A., Goltsman E., Auger S., Galleron N., Segurens B., Dossat C.,
RA Land M.L., Broussolle V., Brillard J., Guinebretiere M.-H., Sanchis V.,
RA Nguen-the C., Lereclus D., Richardson P., Wincker P., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "Extending the Bacillus cereus group genomics to putative food-borne
RT pathogens of different toxicity.";
RL Chem. Biol. Interact. 171:236-249(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000903; ABY41366.1; -; Genomic_DNA.
DR RefSeq; WP_002113316.1; NC_010184.1.
DR AlphaFoldDB; A9VP69; -.
DR SMR; A9VP69; -.
DR STRING; 315730.BcerKBAB4_0097; -.
DR EnsemblBacteria; ABY41366; ABY41366; BcerKBAB4_0097.
DR GeneID; 66264829; -.
DR KEGG; bwe:BcerKBAB4_0097; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_1_9; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000002154; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1177
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000141660"
FT REGION 1154..1177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1158..1177
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1177 AA; 131782 MW; 8EF87F466B2A23FB CRC64;
MTGQLVQYGR HRQRRSYARI SEVLELPNLI EIQTSSYQWF LDEGLREMFQ DISPIEDFTG
NLSLEFIDYS LGEPKYSVEE CKERDVTYAA PLRVKVRLIN KETGEVKEQD VFMGDFPLMT
ETGTFVINGA ERVIVSQLVR SPSVYYSGKV DKNGKRGFTA TVIPNRGAWL EYETDAKDVV
YVRIDRTRKL PVTVLLRALG FGSDQEITEL LGDNEYLSNT LEKDNTDSTE KALLEIYERL
RPGEPPTVEN AKSLLVSRFF DPKRYDLANV GRYKINKKLH IKNRLFNQRL AETLVDPETG
EILAAEGTVL DRRTLDRILP YLEKNIGFKT AKPMGGVVAG DVELQSIKIY APESEGERSI
NVIGNANITR DIKHITPGDI LASISYFFNL LYKVGDTDDI DHLGNRRLRS VGELLQNQFR
IGLSRMERVV RERMSIQDTN AITPQALINI RPVIASIKEF FGSSQLSQFM DQTNPLAELT
HKRRLSALGP GGLTRERAGF EVRDVHYSHY GRMCPIETPE GPNIGLINSL SSFAKVNEFG
FIETPYRRVD PETGLVTGQV DYLTADEEDN YVVAQANMKL SEVGEFLDED IVARFRGENI
VTNKERIDYM DVSPKQVVSA ATACIPFLEN DDSNRALMGA NMQRQAVPLI NPESPIVGTG
MEYVSAKDSG AAVICKQPGV VERVEAREVW VRRYVDVDGQ TVKGDLDRYK MQKFIRSNQG
TCYNQRPIVS VGDQVVKGEI LADGPSMELG ELALGRNVLV GFMTWDGYNY EDAIIMSERL
VKDDVYTSIH IEEYESEARD TKLGPEEITR DIPNVGEDAL RNLDERGIIR VGAEVKDGDL
LVGKVTPKGV TELTAEERLL HAIFGEKARE VRDTSLRVPH GGGGIILDVK VFNREDGDEL
PPGVNQLVRA YIVQKRKISE GDKMAGRHGN KGVISRILPE EDMPYLPDGT PIDIMLNPLG
VPSRMNIGQV LELHLGMAAR YLGIHIATPV FDGAREEDVW GTIEEAGMAN DAKTILYDGR
TGEPFDNRVS VGVMYMIKLA HMVDDKLHAR STGPYSLVTQ QPLGGKAQFG GQRFGEMEVW
ALEAYGAAYT LQEILTVKSD DVIGRVKTYE AIVKGENVPE PGVPESFKVL IKELQSLGMD
VKMMSINDTE IEMRDTEDDD DHQSADKLNV EVETTKE
