ID   RPOB_BACP2              Reviewed;        1196 AA.
AC   A8F976;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BPUM_0093;
OS   Bacillus pumilus (strain SAFR-032).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=315750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032;
RX   PubMed=17895969; DOI=10.1371/journal.pone.0000928;
RA   Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D.,
RA   Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H.,
RA   Lee S., Nazareth L., Blyth P., Holder M., Buhay C., Tirumalai M.R., Liu Y.,
RA   Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P.,
RA   Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D.,
RA   Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F.,
RA   Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K.,
RA   Weinstock G.M.;
RT   "Paradoxical DNA repair and peroxide resistance gene conservation in
RT   Bacillus pumilus SAFR-032.";
RL   PLoS ONE 2:E928-E928(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000813; ABV60793.1; -; Genomic_DNA.
DR   AlphaFoldDB; A8F976; -.
DR   SMR; A8F976; -.
DR   STRING; 315750.BPUM_0093; -.
DR   EnsemblBacteria; ABV60793; ABV60793; BPUM_0093.
DR   KEGG; bpu:BPUM_0093; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_1_9; -.
DR   OMA; FMTWEGY; -.
DR   Proteomes; UP000001355; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1196
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329166"
FT   REGION          1155..1196
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1161..1175
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1196 AA;  133964 MW;  BBD516E9334353A4 CRC64;
     MNQLTGQLVQ YGRHRQRRSY ARISEVLELP NLIEIQTSSY QWFLDEGLRE MFQDISPIED
     FTGNLSLEFI DYSLGDPKYP VAESKERDVT YSAPLRVKVR LINKETGEVK DQDVFMGDFP
     IMTDTGTFII NGAERVIVSQ LVRSPSVYFS GKVDKNGKKG FTATVIPNRG AWLEYETDAK
     DVVYVRIDRT RKLPVTVLLR ALGFSSDQEI LDLIGENEYL RNTLEKDNTE NADKALLEIY
     ERLRPGEPPT VENAKSLLDS RFFDPKRYDL ANVGRYKINK KLHIKNRLFN QKLAETLVDP
     ETGEILAEKG QILDRRVLDK VLPYLENGIG FRKLYPNGGV VEDEVELQSI KIYAPSDQEG
     EQVINVIGNA YVEEAVKNIT PSDIIASISY FFNLLHGVGD TDDIDHLGNR RLRSVGELLQ
     NQFRIGLSRM ERVVRERMSI QDTNTITPQQ LINIRPVIAS IKEFFGSSQL SQFMDQTNPL
     AELTHKRRLS ALGPGGLTRE RAGMEVRDVH YSHYGRMCPI ETPEGPNIGL INSLSSFAKV
     NRFGFIETPY RRVDPETGKV TPRIDYLTAD EEDNYVVAQA NALLADDGSF IDDNIIARFR
     GENTVVPRNR VDYMDVSPKQ VVSAATACIP FLENDDSNRA LMGANMQRQA VPLMQPESPI
     VGTGMEYVSG KDSGAAVICR YPGVVERVEA KNIWVRRYED VDGQQVKGNL DKYSLLKFVR
     SNQGTCYNQR PIVSVGDEVV KGEILADGPS MEKGELALGR NVMVGFMTWD GYNYEDAIIM
     SERLVKDDVY TSIHIEEYES EARDTKLGPE EITRDIPNVG EDALRNLDER GIIRIGAEVK
     DGDLLVGKVT PKGVTELTAE ERLLHAIFGE KAREVRDTSL RVPHGGGGII HDVKVFNRED
     GDELPPGVNQ LVRVYIVQKR KISEGDKMAG RHGNKGVISK ILPEEDMPYL PDGTPIDIML
     NPLGVPSRMN IGQVLELHMG MAARYLGIHI ASPVFDGARE EDVWETLEEA GMSRDAKTVL
     YDGRTGEPFD NRVSVGIMYM IKLAHMVDDK LHARSTGPYS LVTQQPLGGK AQFGGQRFGE
     MEVWALEAYG AAYTLQEILT VKSDDVVGRV KTYEAIVKGD NVPEPGVPES FKVLIKELQS
     LGMDVKILSG DEEEIEMRDL EDDEETKKAD GLALSNDEDA ADLAPVDLER DAVTKE