RPOB_BACTN
ID RPOB_BACTN Reviewed; 1270 AA.
AC Q8A469;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BT_2734;
OS Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=226186;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC VPI-5482 / E50;
RX PubMed=12663928; DOI=10.1126/science.1080029;
RA Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA Hooper L.V., Gordon J.I.;
RT "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL Science 299:2074-2076(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AE015928; AAO77840.1; -; Genomic_DNA.
DR RefSeq; NP_811646.1; NC_004663.1.
DR RefSeq; WP_008762029.1; NZ_UYXG01000001.1.
DR AlphaFoldDB; Q8A469; -.
DR SMR; Q8A469; -.
DR STRING; 226186.BT_2734; -.
DR PaxDb; Q8A469; -.
DR PRIDE; Q8A469; -.
DR EnsemblBacteria; AAO77840; AAO77840; BT_2734.
DR GeneID; 45362450; -.
DR GeneID; 60923905; -.
DR KEGG; bth:BT_2734; -.
DR PATRIC; fig|226186.12.peg.2777; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_10; -.
DR InParanoid; Q8A469; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000001414; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1270
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047861"
SQ SEQUENCE 1270 AA; 142488 MW; C947CF309AF52A00 CRC64;
MSSNTVNQRV NFASTKNPLE YPDFLEVQLK SFQDFLQLDT PPEKRKNEGL YKVFAENFPI
ADTRNNFVLE FLDYYIDPPR YTIDDCIERG LTYSVPLKAK LKLYCTDPDH EDFDTVIQDV
FLGPIPYMTD KATFVINGAE RVVVSQLHRS PGVFFGQSVH ANGTKLYSAR IIPFKGSWIE
FATDINNVMY AYIDRKKKLP VTTLLRAIGF ENDKDILEIF NLAEDVKVNK TNLKRVLGRK
LAARVLKTWI EDFVDEDTGE VVSIERNEVI IDRETVLEEV HIDEILESGV QNILLHKDEP
NQSDFSIIYN TLQKDPSNSE KEAVLYIYRQ LRNADPADDA SAREVINNLF FSEKRYDLGD
VGRYRINKKL NLTTDMDVRV LTKEDIIEII KYLIELINSK ADVDDIDHLS NRRVRTVGEQ
LSNQFAVGLA RMSRTIRERM NVRDNEVFTP IDLINAKTIS SVINSFFGTN ALSQFMDQTN
PLAEITHKRR MSALGPGGLS RERAGFEVRD VHYTHYGRLC PIETPEGPNI GLISSLCVFA
KINELGFIET PYRKVENGKV DLSDNGLVYL TAEEEEEKII AQGNAPLNDD GTFVRNKVKS
RQDADFPVVE PSEVDLMDVS PQQIASIAAS LIPFLEHDDA NRALMGSNMM RQAVPLLRSE
APIVGTGIER QLVRDSRTQI TAEGDGVVDF VDATTIRILY DRTEDEEFVS FEPALKEYRI
PKFRKTNQNM TIDLRPICDK GQRVKKGDIL TEGYSTEKGE LALGKNLLVA YMPWKGYNYE
DAIVLNERVV REDLLTSVHV EEYSLEVRET KRGMEELTSD IPNVSEEATK DLDENGIVRI
GARIEPGDIM IGKITPKGES DPSPEEKLLR AIFGDKAGDV KDASLKASPS LKGVVIDKKL
FSRVIKNRSS KLADKALLPK IDDEFESKVA DLKRILVKKL MTLTEGKVSQ GVKDYLGAEV
IAKGSKFSAS DFDSLDFTSI QLSNWTSDDH TNGMVRDLVM NFIKKYKELD AELKRKKFAI
TIGDELPAGI IQMAKVYIAK KRKIGVGDKM AGRHGNKGIV SRVVRQEDMP FLADGTPVDI
VLNPLGVPSR MNIGQIFEAV LGRAGKTLGV KFATPIFDGA TMDDLDQWTD KAGLPRYCKT
YLCDGGTGEQ FDQAATVGVT YMLKLGHMVE DKMHARSIGP YSLITQQPLG GKAQFGGQRF
GEMEVWALEG FGAAHILQEI LTIKSDDVVG RSKAYEAIVK GEPMPQPGIP ESLNVLLHEL
RGLGLSINLE
