RPOB_BDEBA
ID RPOB_BDEBA Reviewed; 1412 AA.
AC Q6MJ09;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Bd2984;
OS Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 /
OS HD100).
OC Bacteria; Proteobacteria; Oligoflexia; Bdellovibrionales;
OC Bdellovibrionaceae; Bdellovibrio.
OX NCBI_TaxID=264462;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100;
RX PubMed=14752164; DOI=10.1126/science.1093027;
RA Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C.,
RA Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E.,
RA Schuster S.C.;
RT "A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a
RT genomic perspective.";
RL Science 303:689-692(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAE80754.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BX842654; CAE80754.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; Q6MJ09; -.
DR SMR; Q6MJ09; -.
DR STRING; 264462.Bd2984; -.
DR PRIDE; Q6MJ09; -.
DR EnsemblBacteria; CAE80754; CAE80754; Bd2984.
DR KEGG; bba:Bd2984; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_7; -.
DR Proteomes; UP000008080; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1412
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000224032"
SQ SEQUENCE 1412 AA; 157078 MW; C87817DDDB3F84FD CRC64;
MSDFLITEST LEKTPVTASN IRVRKSFAKN KQVIDIPNLI ELQKSSYEAF IQKDMDPDRR
GDAGLNGVFK SVFPITDFNN TASLEFVSYT LEPAKYDVDE CRQRGMTFAA PIKVTLRLIV
FDVDEETEAR SIRDVKEQEV YLGEIPLMTA NGSFIINGTE RVVVSQLHRS PGVFFDHDGG
KNNASGKLIY SARVIPYRGS WLDAEFDQKD LIHVRIDRRR KFPVTILLKA LGYNSEQLLE
YFYDLDEVYV KGGKLFRKLD IERMSGQRAL TDIVDPKTGE ALVKAGRRIT RAIVKKIKDL
DITELDVLPE DLEGKVLAKP LIDESTGEII ADANAELNAA IIKRSIEAGI ESFFMIFFDG
LTVGPYLRNT LLVDKVSNKE ESLVEIYKRL RPGEPPTLEA ATTFFGRLFF DPETYDLSEV
GRIKINHRFG ISMDECPPSH RTLTHKDILS TIKTLIDLKN GRGVIDDIDH LGNRRVRSVG
ELLENQYRIG LVRMERAIRE RMSLQDVETM MPHDLVNAKP VNAVVKEFFG SSQLSQFMDQ
TNPLSEITHK RRLSALGPGG LTRDRAGFEV RDVHPTHYGR ICPIETPEGP NIGLIASLAT
YARINNYGFI ETPYRKVEAG QVSKDINYLS ALEEAGHHIA PAARDEAGNK AIQTATTITR
RDGEYEIVDK DKVALMDVSP SQLVSIAASL IPFLEHDDAN RALMGSNMQR QAVPLLRSRA
PLVGTGVERL VARDSGTSIV AQNDGIVEEV DASRIVIRRF AKGGELGANV DIYNLTKYQR
TNQNTCFNQK PIVTVGDKIS KGDIIADGPS TELGELALGQ NILVAFTPWQ GYNFEDSILI
SERLLKDDVY TSIHIEEFEC VARDTKLGKE EITRDIANVG EEALKDLDSS GIIRIGAEVR
PGFILVGKVT PKGETQLSPE EKLLRAIFGE KAGDVRDTSL RAPSGVYGTV IDAQVYSREG
ADRDERLSLI IEEKKRKLEK DLAVEQNVIK NNAISKLRDI LVGKITTGVL LNEDGSQKLL
NKGQEITEAD LETVPFELLN YIPLEQDLEF QVNKIIDNAR NQLDAVKLVF NEKIDRLRKG
DELPPGVIKM VKVYVAIKRK MQVGDKFAGR HGNKGVVSKV LPAEDMPYLA DGTPVDMVLN
PLGVPSRMNI GQILEVHLGW AAHNLGKQLG AHLEKWNAEN ARTEMKDIFS DSDISAKLDK
ADDASLKSMV QRMKHGIHVG TPVFDGARES DVKGLLAKAQ VPSSGKSVLF DGRTGEPFTN
PVTVGIMYML KLHHLVEEKI HARSIGPYSL VSQQPLGGKA QFGGQRLGEM EVWAIEAYGA
AYSLQEFLTV KSDDVAGRTR MYESIVKGEN ILEPGLPESF NVLVKELQSL ALNVELMESD
ILRDQDEDFG DEEVIEVEPV APVASKDEPE QH
