RPOB_BLOFL
ID RPOB_BLOFL Reviewed; 1342 AA.
AC Q7VRP7;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Bfl557;
OS Blochmannia floridanus.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; ant endosymbionts; Candidatus Blochmannia.
OX NCBI_TaxID=203907;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12886019; DOI=10.1073/pnas.1533499100;
RA Gil R., Silva F.J., Zientz E., Delmotte F., Gonzalez-Candelas F.,
RA Latorre A., Rausell C., Kamerbeek J., Gadau J., Hoelldobler B.,
RA van Ham R.C.H.J., Gross R., Moya A.;
RT "The genome sequence of Blochmannia floridanus: comparative analysis of
RT reduced genomes.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:9388-9393(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX248583; CAD83239.1; -; Genomic_DNA.
DR RefSeq; WP_011126815.1; NC_005061.1.
DR AlphaFoldDB; Q7VRP7; -.
DR SMR; Q7VRP7; -.
DR STRING; 203907.Bfl557; -.
DR PRIDE; Q7VRP7; -.
DR EnsemblBacteria; CAD83239; CAD83239; Bfl557.
DR KEGG; bfl:Bfl557; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_6; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000002192; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 2.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 2.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1342
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047876"
SQ SEQUENCE 1342 AA; 151237 MW; A408FF1861473A06 CRC64;
MVFSYTEKKR IRKDFGKRPQ VLDIPYLLSI QLGSFQKFVK KDPEGQNGLE SAFRSVFPIK
SYNGNAELRY LDYKLGEPVF DVKECQIRGM TFAAPLRVQL CLVIYERDGS AYNTIKRTQQ
QEVYMGEIPL MTDNGTFIIN GIERVVVSQL HRSPGVFFDS DKGKTHSSGK ILYTARIIPY
RGSWLDFEFD LKDNLFVRID RRRKLPVTVL LRALDYTNDQ ILNTFFNTVV YEFKDDLLFM
NLVPERLRGE TVSFDIKAND IIYVERGRRI TSKHINKLKA DNIIKVEVPL NYLMGKVIIK
DYLDIKNGEL IVAANTEISM DILHNLVKSG FNIIETLFSN DLDYGNYISE TLRIDSTVTR
FDALVEIYRV MRPGEPPTKE AAEYLFESLF FLEERYDLSD VGRMKFNRSL QRDDISGSGV
LTKNDIIDVI KKLIDIRNGK GDVDDIDHLG NRRIRSVGEM AENQFRIGLV RVERAVKERL
SLGDLDVLTP QDLINAKPIS AAVREFFNSS QLSQFMDQNN PLSEITHKRR ISALGPGGLT
RERAGFEVRD VHPTHYGRVC PIETPEGPNI GLINSLSVYA RTNKYGFLET PYRRVCDGKV
SNDIHYLSAI EEGDFIIAQA NTNLNKDGQF IDDLITCRNK GESGLFRKDQ IDYMDVSTQQ
IVSVAASLIP FLEHDDANRA LMGANMQRQA VPVIRSEKPL VGTGMERSVA VDSGVTVVAK
RGGMVKYVDA SRIVIHVNVN EISVEEAGID IYNLKKYVRS NQNTCINQRP CVELGELIKK
GDVIADGPST DLGELALGQN MRIAFMPWNG YNFEDSMLVS ERVVQEDRFT SIHIQELSCI
SRDTKLGPEE ITSDIPNIGE TALSKLDEVG VVYIGAEVVG GDILVGKITP KGETQLTPEE
KLLRAIFGEK ASDVKDSSLR VPNGICGTVI DVQIFTRDGI KKDKRALNIE LTKLNQIKKD
LGEELKIFES ALFDRVYALL ISSGVKKESL LDMNRCAWLN LKLKDITKQN QLLQLSHQYL
DLKRIFEEKI SIQYQKITQG DELAPGILKV VKVYLAVKRQ IQPGDKMAGR HGNKGVISKI
NPVEDMPYDE YGVPVDIVLN PLGVPSRMNI GQILETHLGM AAKGIGDQIN LMLQQHQEIN
KLRKFIQKAY KLGVGVRQNI DLNTFSDLEV LRLAKNLKSG MPIATPVFDG ATEQEIKELL
QLSGLPTSGQ ITLFDGCTGE VFERKVTVGY MYMLKLNHLV DDKMHARSTG SYSLVTQQPL
GGKAQFGGQR FGEMEVWALE AYGASYTLQE MLTVKSDDVN GRTKMYKNII DGNHMMEPGM
PESFNVLLKE IRSLAINIEL DD
