ID   RPOB_BRADU              Reviewed;        1372 AA.
AC   Q89J74;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=bll5410;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; BA000040; BAC50675.1; -; Genomic_DNA.
DR   RefSeq; NP_772050.1; NC_004463.1.
DR   RefSeq; WP_011088159.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89J74; -.
DR   SMR; Q89J74; -.
DR   STRING; 224911.27353685; -.
DR   EnsemblBacteria; BAC50675; BAC50675; BAC50675.
DR   GeneID; 64025174; -.
DR   KEGG; bja:bll5410; -.
DR   PATRIC; fig|224911.44.peg.5310; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   InParanoid; Q89J74; -.
DR   OMA; FMTWEGY; -.
DR   PhylomeDB; Q89J74; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1372
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047869"
SQ   SEQUENCE   1372 AA;  153276 MW;  2F30842AC18F1B7E CRC64;
     MAQQTFTGRK RVRKFFGHIK EVAEMPNLIE VQKASYDQFL MVDEPQGGRL DEGLQAVFRS
     VFPISDFSGT SMLEFVRYEF EQPKYDVDEC RQRGMTFAAP LKVTLRLIVF DIDEETGAKS
     VKDIKEQDVY MGDIPLMTMN GTFIVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA
     ARVIPYRGSW LDIEFDAKDI VYARIDRRRK IPVTSLMFAL GLDGEAILST FYKKILYKRT
     KEGWRVPFDA NRFRGYSTIN DLIDADTGKV VLEAGKKLTV RAARQLQEKG LKALRLSDEE
     LVGNYLAEDL VNPKTGEIHA EAGEEITDKN MKALNEQGYK ELPLLDIDHV NVGAYIRNTL
     SADKNMTRED ALFDIYRVMR PGEPPTLDSA QAMFQSLFFD AERYDLSAVG RVKMNMRLDL
     DAPDTQRTLR KEDILSVIKT LVDLRDGKGE IDDIDHLGNR RVRSVGELME NQYRIGLLRM
     ERAIKERMSS VDIDTVMPQD LINAKPAAAA VREFFGSSQL SQFMDQTNPL SEITHKRRLS
     ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL INSLATFARV NKYGFVETPY
     RKVKDGRVTD EVVYLSAMEE GRYTVAQANV PLDPKGRFTE DLVVCRHAGE VLPVTPDKVD
     YMDVSPKQLV SVAAALIPFL ENDDANRALM GSNMQRQAVP LVRAEAPFVG TGMEGVVARD
     SGAAIAARRS GVIDQIDATR VVIRATEDLD PTKSGVDIYR LMKYQRSNQS TCINQRPLVK
     VGDIVKKGDI IADGPSTDLG ELALGRNVLV AFMPWNGYNF EDSILLSERI VKEDVFTSIH
     IEEFEVMARD TKLGPEEITR DIPNVSEEAL KNLDEAGIVY IGAEVRAGDI LVGKITPKGE
     SPMTPEEKLL RAIFGEKASD VRDTSLRVPP GVQGTIVEVR VFNRHGVDKD ERALAIEREE
     IERLAKDRDD EQAILDRNVY NRLAELLEGR QGIAGPKGFK KDTKITRAVL EEYPKSQWWL
     FASPNDKLMA EIEAMRKQYD ESKKGLEQRF LDKVEKLQRG DELPPGVMKM VKVFVAVKRK
     IQPGDKMAGR HGNKGVVSKI VPIEDMPFLE DGTHADIVLN PLGVPSRMNV GQILETHLGW
     ACAGLGKRIG QTVDAYLSKQ DIKPLKETLK KVYGEDETIK SLNDNELIEL GHNLSRGVPI
     ATPVFDGAKE ADIEEMLKLA GLDASGQSTV YDGRTGDPFD RKVTVGYIYM LKLHHLVDDK
     IHARSIGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEMLT VKSDDVAGRT
     KVYEAIVRGD DTFEAGIPES FNVLVKEMRS LGLNVDLHNS KMGPAPTSEA AE