RPOB_BRASB
ID RPOB_BRASB Reviewed; 1372 AA.
AC A5ELN7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BBta_5081;
OS Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX NCBI_TaxID=288000;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BTAi1 / ATCC BAA-1182;
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000494; ABQ37081.1; -; Genomic_DNA.
DR RefSeq; WP_012045052.1; NC_009485.1.
DR AlphaFoldDB; A5ELN7; -.
DR SMR; A5ELN7; -.
DR STRING; 288000.BBta_5081; -.
DR PRIDE; A5ELN7; -.
DR EnsemblBacteria; ABQ37081; ABQ37081; BBta_5081.
DR KEGG; bbt:BBta_5081; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000246; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1372
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300286"
SQ SEQUENCE 1372 AA; 153408 MW; FA9851F39BBD4F55 CRC64;
MAQQTFTGRK RVRKFFGHIK EVAEMPNLIE VQKASYDQFL MVDEPTGGRP DEGLQAVFRS
VFPISDFSGT SMLEFVRYEF EPPKYDVDEC RQRGMTFAAP LKVTLRLIVF DIDEETGAKS
VKDIKEQDVY MGDIPLMTMN GTFIVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA
ARVIPYRGSW LDIEFDAKDI VFARIDRRRK IPVTSLMFAL GLDGEQILST FYKKLIYKRI
KEGWRVPFDA NRFRGYSTVN DLIDADTGKV VLEAGKKLTV RTARQLQEKG LKALRMSDEE
LLGNYIAEDL VNPKTGEIYA EAGEEITDKL FKVLNEQGYK DLPLLDIDHV NVGPYIRNTL
SADKNMTRED ALFDIYRVMR PGEPPTLDSA QAMFQSLFFD AERYDLSAVG RVKMNMRLEL
DAPDTQRTLR KEDILAVIKT LVDLRDGKGE IDDIDHLGNR RVRSVGELME NQYRIGLLRM
ERAIKERMSS VDIDTVMPQD LINAKPAAAA VREFFGSSQL SQFMDQTNPL SEITHKRRLS
ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL INSLATFARV NKYGFVETPY
RKVRDGRVTD EVVYLSAMEE GRYRVAQANV PLDAKGRFTE DLVVCRHAGE VVPMTPDKVD
YMDVSPKQLV SVAAALIPFL ENDDANRALM GSNMQRQAVP LVRAEAPFVG TGMEGVVARD
SGAAIAARRS GVVDQIDATR VVIRATEDLD PTKSGVDIYR LMKFQRSNQS TCINQRPLVK
VGDIVKKGDI IADGPSTDLG ELALGRNVLV AFMPWNGYNF EDSILLSERI VKEDVFTSIH
IEEFEVMARD TKLGPEEITR DIPNVSEEAL KNLDEAGIVY IGAEVRAGDI LVGKITPKGE
SPMTPEEKLL RAIFGEKASD VRDTSLRVPP GVQGTIVEVR VFNRHGVDKD ERALAIEREE
IERLAKDRDD EQAILDRNVY SRLADMLDGR QGISGPKGFK KDTKITRAVL DEYPKSQWWL
FASANDKLMA EIEAMRKQYD ESKKGLEQRF LDKVEKLQRG DELPPGVMKM VKVFVAVKRK
IQPGDKMAGR HGNKGVVSKI VPIEDMPFLE DGTHADIVLN PLGVPSRMNV GQILETHLGW
ACAGLGKRIA QTVDAYLSKQ DVKPLKETLK RIYGEDETIK SLNDNELLEL GHNLSRGVPI
ATPVFDGAKE ADIEEMLKLA GMDASGQSTV YDGRTGDPFD RKVTVGYIYM LKLHHLVDDK
IHARSIGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEMLT VKSDDVAGRT
KVYEAIVRGD DTFEAGIPES FNVLVKEMRS LGLNVDLHNS KLGPAPTSEA AE
