RPOB_BRASO
ID RPOB_BRASO Reviewed; 1372 AA.
AC A4YSI1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BRADO3054;
OS Bradyrhizobium sp. (strain ORS 278).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX NCBI_TaxID=114615;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ORS 278;
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CU234118; CAL76857.1; -; Genomic_DNA.
DR RefSeq; WP_011926045.1; NC_009445.1.
DR AlphaFoldDB; A4YSI1; -.
DR SMR; A4YSI1; -.
DR STRING; 114615.BRADO3054; -.
DR EnsemblBacteria; CAL76857; CAL76857; BRADO3054.
DR KEGG; bra:BRADO3054; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR BioCyc; BSP114615:BRADO_RS14310-MON; -.
DR Proteomes; UP000001994; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1372
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000300287"
SQ SEQUENCE 1372 AA; 153378 MW; 96FC0005273C9C32 CRC64;
MAQQTFTGRK RVRKFFGHIK EVAEMPNLIE VQKASYDQFL MVDEPTGGRP DEGLQAVFRS
VFPISDFSGT SMLEFVRYEF EPPKYDVDEC RQRGMTFAAP LKVTLRLIVF DIDEETGAKS
VKDIKEQDVY MGDIPLMTMN GTFIVNGTER VIVSQMHRSP GVFFDHDKGK THSSGKLLFA
ARVIPYRGSW LDIEFDAKDI VFARIDRRRK LPVTSLMFAL GLDGEQILAT FYKKLIYKRI
KEGWRVPFDA NRFRGYSTVN DLIDADTGKV VLEAGKKLTV RAARQLQEKG LKALRMSDEE
LLGNYIAEDL VNPKTGEIYA EAGEEITDKL FKVLNEQGYK DLPLLDIDHV NVGPYIRNTL
SADKNMTRED ALFDIYRVMR PGEPPTLDSA QAMFQSLFFD AERYDLSAVG RVKMNMRLEL
DAPDTQRTLR KEDILAVIKT LVDLRDGKGE IDDIDHLGNR RVRSVGELME NQYRIGLLRM
ERAIKERMSS VDIDTVMPQD LINAKPAAAA VREFFGSSQL SQFMDQTNPL SEITHKRRLS
ALGPGGLTRE RAGFEVRDVH PTHYGRICPI ETPEGPNIGL INSLATFARV NKYGFVETPY
RKVKDGRVTD EVVYLSAMEE GRYRVAQANV PLDAKGRFTE DLVVCRHAGE VVPMTPDKVD
YMDVSPKQLV SVAAALIPFL ENDDANRALM GSNMQRQAVP LVRAEAPFVG TGMEGVVARD
SGAAIAARRS GVIDQIDATR VVIRATEDLD PTKSGVDIYR LMKFQRSNQS TCINQRPLVK
VGDIVKKGDI IADGPSTDLG ELALGRNVLV AFMPWNGYNF EDSILLSERI VKEDVFTSIH
IEEFEVMARD TKLGPEEITR DIPNVSEEAL KNLDEAGIVY IGAEVRAGDI LVGKITPKGE
SPMTPEEKLL RAIFGEKASD VRDTSLRVPP GVQGTIVEVR VFNRHGVDKD ERALAIEREE
IERLAKDRDD EQAILDRNVY SRLADMLDGR QGISGPKGFK KDTKITRAVL DEYPKSQWWL
FAASNDKLMA EIEAMRKQYD ESKKGLEQRF LDKVEKLQRG DELPPGVMKM VKVFVAVKRK
IQPGDKMAGR HGNKGVVSKI VPIEDMPFLE DGTHADIVLN PLGVPSRMNV GQILETHLGW
ACAGLGKRIA QTVDAYLSKQ DVKPLKETLK RIYGEDETIK TLNDNELLEL GHNLSRGVPI
ATPVFDGAKE SDIEEMLKLA GMDASGQSTV YDGRTGDPFD RKVTVGYIYM LKLHHLVDDK
IHARSIGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEMLT VKSDDVAGRT
KVYEAIVRGD DTFEAGIPES FNVLVKEMRS LGLNVDLHNS KLGPAPTSEA AE