RPOB_BRUA4
ID RPOB_BRUA4 Reviewed; 1377 AA.
AC A6X0A9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Oant_1947;
OS Brucella anthropi (strain ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 /
OS LMG 3331 / NBRC 15819 / NCTC 12168 / Alc 37) (Ochrobactrum anthropi).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=439375;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49188 / DSM 6882 / CCUG 24695 / JCM 21032 / LMG 3331 / NBRC
RC 15819 / NCTC 12168 / Alc 37;
RX PubMed=21685287; DOI=10.1128/jb.05335-11;
RA Chain P.S., Lang D.M., Comerci D.J., Malfatti S.A., Vergez L.M., Shin M.,
RA Ugalde R.A., Garcia E., Tolmasky M.E.;
RT "Genome of Ochrobactrum anthropi ATCC 49188 T, a versatile opportunistic
RT pathogen and symbiont of several eukaryotic hosts.";
RL J. Bacteriol. 193:4274-4275(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000758; ABS14663.1; -; Genomic_DNA.
DR RefSeq; WP_010659907.1; NC_009667.1.
DR AlphaFoldDB; A6X0A9; -.
DR SMR; A6X0A9; -.
DR STRING; 439375.Oant_1947; -.
DR EnsemblBacteria; ABS14663; ABS14663; Oant_1947.
DR GeneID; 61317594; -.
DR KEGG; oan:Oant_1947; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR PhylomeDB; A6X0A9; -.
DR Proteomes; UP000002301; Chromosome 1.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1377
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000051972"
SQ SEQUENCE 1377 AA; 153536 MW; 5499CC9C6979D6C1 CRC64;
MAQTHSFNGR KRVRKFFGKI PEVAEMPNLI EVQKASYDQF LMVEEPSGGR SDEGLQAVFK
SVFPIQDFSG ASMLEFVRYE FDAPKFDVDE CRQRDLTYSA PLKVTLRLIV FDIDEDTGAK
SIKDIKEQDV YMGDMPLMTD NGTFIVNGTE RVIVSQMHRS PGVFFDHDKG KTHSSGKLLF
AARVIPYRGS WLDIEFDSKD IVYARIDRRR KLPATTLLMA LGMDGEDILS TFYKTVTYTR
DGDNWRIPYS ADRFKGMKII SDLIDADTGE VVLEAGKKLT ARSAKQLAEK GLKAIKATED
DLFGSYLAED VVNYATGEIY LEAGDEIDEK VLKTLIDTGE TEINVLDIDH INIGGYIRNT
LAVDKNESRQ EALFDIYRVM RPGEPPTMDS AEAMFNSLFF DAERYDLSAV GRVKMNMRLD
LDAEDTVRVL RKEDILAVVK MLVELRDGRG EIDDIDNLGN RRVRSVGELM ENQYRVGLLR
MERAIKERMS SIEIDTVMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP LSEITHKRRL
SALGPGGLTR ERAGFEVRDV HPTHYGRICP IETPEGPNIG LINSLATFAR VNKYGFIESP
YRKVVDGKVT NDVVYLSAME EAKHSVAQAN VELDERGGFV DEFVICRHAG EVMMAPRENV
DLMDVSPKQL VSVAAALIPF LENDDANRAL MGSNMQRQAV PLVRAEAPFV GTGMEAVVAR
DSGAAIAARR GGVVDQVDAT RIVIRATEDL DPSKSGVDIY RLMKFQRSNQ NTCINQRPLV
RVGDPIQKGD IIADGPSTDL GDLALGRNVL VAFMPWNGYN YEDSILLSEK IVSDDVFTSI
HIEEFEVAAR DTKLGPEEIT RDIPNVSEEA LKNLDEAGIV YIGAEVHPGD ILVGKITPKG
ESPMTPEEKL LRAIFGEKAS DVRDTSMRMP PGTYGTVVEV RVFNRHGVEK DERAMAIERE
EIERLAKDRD DEQAILDRNV YGRLADMIDG KVAAAGPKGF KKGSTITREL MTEYPRSQWW
QFAVDDEKLQ GELEALRGQY DESKKLLEAR FMDKVEKVQR GDEMPPGVMK MVKVFVAVKR
KIQPGDKMAG RHGNKGVVSR ILPVEDMPFL EDGTHADIVL NPLGVPSRMN VGQILETHLG
WACAGMGKKI GELIDVYRKT ANIEPLRDTL EHIYPDNDRN EPVRSYDDDS VLMLANQVKR
GVSIATPVFD GAVEADINAM LTDAGLATSG QSTLYDGRTG EPFDRQVTMG YIYMLKLHHL
VDDKIHARSI GPYSLVTQQP LGGKAQFGGQ RFGEMEVWAL EAYGAAYTLQ EMLTVKSDDV
AGRTKVYEAI VRGDDTFEAG IPESFNVLVK EMRSLGLNVE LDDTRDEQQQ ALPDAAE
