ID   RPOB_BUCAP              Reviewed;        1342 AA.
AC   P41184;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=BUsg_035;
OS   Buchnera aphidicola subsp. Schizaphis graminum (strain Sg).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=198804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1369199; DOI=10.1007/bf01575863;
RA   Clark M.A., Baumann L., Baumann P.;
RT   "Sequence analysis of an aphid endosymbiont DNA fragment containing rpoB
RT   (beta-subunit of RNA polymerase) and portions of rplL and rpoC.";
RL   Curr. Microbiol. 25:283-290(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sg;
RX   PubMed=12089438; DOI=10.1126/science.1071278;
RA   Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA   Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT   "50 million years of genomic stasis in endosymbiotic bacteria.";
RL   Science 296:2376-2379(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z11913; CAA77970.1; -; Genomic_DNA.
DR   EMBL; AE013218; AAM67606.1; -; Genomic_DNA.
DR   PIR; S32680; S32680.
DR   RefSeq; WP_011053572.1; NC_004061.1.
DR   AlphaFoldDB; P41184; -.
DR   SMR; P41184; -.
DR   STRING; 198804.BUsg_035; -.
DR   PRIDE; P41184; -.
DR   EnsemblBacteria; AAM67606; AAM67606; BUsg_035.
DR   KEGG; bas:BUsg_035; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_3_6; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000000416; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1342
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047873"
SQ   SEQUENCE   1342 AA;  152061 MW;  7E30BC9E013D31ED CRC64;
     MVYSYTEKKR IRKDFGKRPQ VLDIPYLLSI QLDSFKKFIK IDPEGLHGLE AAFRSVFPIC
     GYNGNSELQY VSYRLGDAIF DVKECQIRGA TYSAPLRVRL RLVIYERDVL EPTVKDIKEQ
     EVYMGEIPLM TNNGTFIING TERVVVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR
     GSWLDFEFDP KDNLFVRIDR RRKLPVTIIL RALNYNTEEI LNLFFEKNIF NINNNKIQLE
     LVSERLRGET ASFDIKKNGK IYVKKGRRIT AKHIQELKKD KINSITVPVE YILGRIVSKN
     YLDPKTGETI ILANTELSLE ILTKLKNSSF FSIETLFTND LDHGPYISET LRIDSSHDRI
     SALMEIYRVM RPGEPLTKEA TENLFENLFF SEDRYDLSSV GRMKFNRSLL RKKIEGVSTL
     NKEDIIDVIK KLIDIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRIGLVR VERAVKERLS
     VGDLDTLMPQ DMINAKPISA AIKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGLGGLTR
     ERAGFEVRDV HPTHYGRVCP IETPEGPNIG LINSLSVYAR TNSYGFLETP YRKVHNRLVT
     DEIHYLSAIE EGNYVIAQAN TNIDKNNYFI DDLVTCRHKG ESSLFNCNQV DYMDVSTQQI
     VSVGASLIPF LEHDDANRAL MGANMQRQAV PTLKTDKPLV GTGMERAVAV DSGVTVVAKR
     GGIIQYIDAS RIIIKVNEEE TYTGEAGIDI YNLTKYTRSN QNTCINQKPC VKLREKINKN
     DVLADGPSTD LGELALGQNM RVAFMPWNGY NFEDSILVSE KVVQEDRFTT IHIQELSCIS
     RDTKLGPEEI SSDIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK
     LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQIFTRDGVK KDKRALEIEN MQLKKAKKDL
     TEEFKIFELS LFSRIKKTLV SFNIKEDFLN KLPYEKWFKI DIQDRNKKKE IEKLLQQHNQ
     LKKEFEKKIE VKRRKITQGD DLAPGVLKIV KVYLAVKRQI QPGDKMAGRH GNKGVISKIN
     PVEDMPYDEN GIPVDIVLNP LGVPSRMNIG QILETHLGMA AKGIGNKINN MLKKQEKISN
     LKKFIQKAFD LGENLRQKVN LDDFSNEEIL DLAKNLKKGM PISTPVFDGA QENEIKQMLK
     FSNLPTSGQI SLFDGRTGEK FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG
     GKAQFGGQRF GEMEVWALEA YGASYTLQEM LTVKSDDVNG RTKMYKNIVD GNHQMEPGMP
     ESFNVLLKEI RSLGINIELE NE