RPOB_BUCBP
ID RPOB_BUCBP Reviewed; 1343 AA.
AC Q89B20;
DT 30-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2003, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=bbp_035;
OS Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Erwiniaceae; Buchnera.
OX NCBI_TaxID=224915;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bp;
RX PubMed=12522265; DOI=10.1073/pnas.0235981100;
RA van Ham R.C.H.J., Kamerbeek J., Palacios C., Rausell C., Abascal F.,
RA Bastolla U., Fernandez J.M., Jimenez L., Postigo M., Silva F.J.,
RA Tamames J., Viguera E., Latorre A., Valencia A., Moran F., Moya A.;
RT "Reductive genome evolution in Buchnera aphidicola.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:581-586(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AE016826; AAO26778.1; -; Genomic_DNA.
DR RefSeq; WP_011091179.1; NC_004545.1.
DR AlphaFoldDB; Q89B20; -.
DR SMR; Q89B20; -.
DR STRING; 224915.bbp_035; -.
DR PRIDE; Q89B20; -.
DR EnsemblBacteria; AAO26778; AAO26778; bbp_035.
DR GeneID; 56470579; -.
DR KEGG; bab:bbp_035; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_3_6; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000601; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1343
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047874"
SQ SEQUENCE 1343 AA; 151550 MW; 35A7FF57BC09F121 CRC64;
MVVYSSTEKK RIRKDFGKRP KVLDIPYLLS IQLNSFQKFI KHDPNGIHGL ESAFRSIFPI
RSYNGNSELQ YINYKLGQPI FNVKECQTRG MTYSSPLRVK LRLVIYEKET LDITVKDIKE
QEVYMGEIPL MTTNGTFIIN GTERVVVSQL HRSPGVFFDS DKGKTHSSGK VLYNARIIPY
RGSWLDFEFD PKDNLFFRID RRRKLPISII LKALNYNTEQ ILDTFFKKNI FKFTENSILM
KLVPHRLRGE TASFDICIDN KTYIKKGQRI TARHINILKE KKIHTIKVPT EYLIGKISAK
NYFNKKTNEI IVSANTELNI NIIKELKNNE YTNIETIFTN DLDHGPYISE TLNIDPTHDK
LSALVEIYRM MRPGEPPTRE AAENLFENLF FSEERYDLSS VGRMKFNRSL QKQNITERGV
LNQSDIISVI KKLIDIRNGH GEIDDIDHLG NRRVRSVGEM AENQFRIGLI RVERAVKDRL
SLGDLDMLMP QDMINAKPIS AAIKEFFGSS QLSQFMDQNN PLSEITHKRR ISALGLGGLT
RERAGFEVRD VHPTHYGRVC PIETPEGPNI GLINSLSVYA QTNSYGFLET PYRKIHNGKV
TNEIKYLSAI EEGNFIIAQA NTNLDETGAF VEDLITCRYK GESSLFNKNQ INYMDVSTQQ
IVSVGASLIP FLEHDDANRA LMGANMQRQA VPTLRSEKPL VGTGMERAVA VDSGVTIIAK
RGGIVQYVDA SRIIIKVKKN EINSEEAGID IYNLKKYTRS NQNTCINQIP CISLGENVEK
GNVLADGPST DLGELALGQN MRVAFMPWNG YNFEDSILIS EKVVQEDRFT TIHIQELACI
SRDTKLGSEE ITSDIPNVGE AALSKLDESG IVYIGAEVNG GDILVGKVTP KGETQLTPEE
KLLRAIFGEK ASDVKDSSLR VPNGVLGTVI DVQIFTRDGV KKDKRALEIE EMQLKQAKKD
LTEEQKVFEL GLFTQIKSTL INSGMNIDQL NKIPKSELFK VKVTNNLYQK KIHKLFLKYE
ELKKCFNKKL ETTRNKITQG DDLAPGVLKI VKVYLAVKRH IQPGDKMAGR HGNKGVISKI
NPIEDMPYDQ NGLPVDVVLN PLGVPSRMNI GQILETHLGL ASKGLGNIID TMLKQQKSIL
NIRKFIQKAF NLGNNTRQKT NLNNFSDDEI LSLAKNFKNG IPISTPVFDG AQESEIKALL
KLADLPSSGQ ITLFDGRTGE KFERPVTVGY MYMLKLNHLV DDKMHARSTG SYSLVTQQPL
GGKAQFGGQR FGEMEVWALE AYGAAYTLQE MLTVKSDDVN GRTKMYKNIV DGNYKMEPGM
PESFNVLLKE IRSLAINIEL ENE
