RPOB_CALBD
ID RPOB_CALBD Reviewed; 1228 AA.
AC B9MQG5;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Athe_0804;
OS Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320)
OS (Anaerocellum thermophilum).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor.
OX NCBI_TaxID=521460;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1888 / DSM 6725 / Z-1320;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C.,
RA Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Kataeva I., Adams M.W.W.;
RT "Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725.";
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP001393; ACM59919.1; -; Genomic_DNA.
DR AlphaFoldDB; B9MQG5; -.
DR SMR; B9MQG5; -.
DR STRING; 521460.Athe_0804; -.
DR EnsemblBacteria; ACM59919; ACM59919; Athe_0804.
DR KEGG; ate:Athe_0804; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_9; -.
DR OMA; FMTWEGY; -.
DR Proteomes; UP000007723; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1228
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000165785"
FT REGION 1175..1204
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1228 AA; 138420 MW; 8841FCE9DAD6875D CRC64;
MALPRPVQYG KVQRMSYGKV KEVLDLPYLL EIQKKSFQWF LDEGLREVLR EISPIKDYSE
TLLLEFVDYY FDGPPKYSEQ ECKERDATYA RPLKVKVRLI NKETGEIKEQ DIYMGEFPIM
TETGTFIING AERVIVSQLI RSPGCYFASS IDKQGRKIFS GTLIPNRGAW LEFETDASEL
LSVRLDRTRK VSLTTLLKAF GLYNQQLIFE KFGEDERLKA SLEKEANKGE LGNPVENALL
EVYRRLRPGE PPNVENARNL LYSMFFDPRR YDLAKVGRYK FNKKLSLWKR IFNKRAAQDV
VDPKTGEILV KEGELITREI ATQIQDAGVN EVWVYADEER PFKVVGNNTV KLDRYVEFDV
SDINIKELVY RPVLDDILNT TNDVLEIKRL IKERERELVP YCLTIDDIFA ATSYFLGLKY
GIGTTDDIDH LGNRRVRAVG ELLQNQFRIG LARMERVIRE RMNIHDIDTV TPQTLINIRP
VTAAIKEFFG SSPLSQFMDQ VNPLAALTNK RRLSALGPGG LSRDRAGFEV RDVHHSHYGR
MCPIETPEGP NIGLITSLAT YARVNEYGFL ETPYRKVDKK EARVTNEVVY LTADEEDTYK
IAQATEPVDE EGRFINQRVT VRFGEEIIEV DKHEVDLIDI SPKQIVSVST SLIPFLENDD
ANRALMGSNM QRQAVPLLMT ESPIIGTGVE YRAAVDSGVC ILAKKDGVVE NVSADEIVIR
NNDGTKDVYH LLKFKRTNQG TCFNQRPIVR KGQEVKAGEV IADGPSTDHG ELALGKNVLV
AFMPWEGYNY EDAILISERL VKEDVYTSIH IEEYECEARD TKLGPEEITR DIPNVGEDAI
KDLDERGIIR IGAEVKSGDI LVGKVTPKGE TELTAEERLL RAIFGEKARE TRDTSLRVPH
GEGGIVVDVK VFSRDKGDEL PPGVNQLVRV YVAQKRKISV GDKMAGRHGN KGVISRILPV
EDMPFLPDGT PVDIVLNPLG VPSRMNIGQI LETHLGYAAK ALGWKVATPV FDGAKEEDIE
EALKLAGLNP TGKTILYDGR TGEPFDNEVT VGYMYMLKLV HLVDDKIHAR STGPYSLVTQ
QPLGGKAQFG GQRFGEMEVW ALEAYGAAYT LQELLTVKSD DVTGRVKTYE AIVKGENIPE
PGIPESFKVL VKELQSLCLD VKLLSEDNKE IELKESVDED EQPQGLGAFE IGGDEIEEDK
EDDKEKFYED LMNATQEDDQ GEIDDIDE
