RPOB_CALS4
ID RPOB_CALS4 Reviewed; 1234 AA.
AC Q8R7U6;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=TTE2301;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; AE008691; AAM25443.1; -; Genomic_DNA.
DR RefSeq; WP_011026346.1; NC_003869.1.
DR AlphaFoldDB; Q8R7U6; -.
DR SMR; Q8R7U6; -.
DR STRING; 273068.TTE2301; -.
DR PRIDE; Q8R7U6; -.
DR EnsemblBacteria; AAM25443; AAM25443; TTE2301.
DR KEGG; tte:TTE2301; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_9; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..1234
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000047986"
FT REGION 1187..1234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1192..1234
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1234 AA; 138644 MW; D8B893E2FC4CABCB CRC64;
MVRPVQAGSR TRMSFAKIDE VLEIPDLIEV QKKSYQWFLE EGLKEVFQEI SPIESFTGNL
ALEFVDYRLE NNPKYSVEEC KDRDTTYAAP LKVKVRLTNR ETGEIKESEV FMGDLPLMTD
KGTFIINGAE RVIVSQLVRS PGVYYDQQFD KFGKKLIFAT VIPNRGAWLE YEEDSNDVIY
VRIDRTRKVP ITVLLRAIGY NTDIQILDLL GEEEKLKATL DKDTTKSEEE ALIEIYKRLR
PGEPPTVESA RSLFNALFFD PKRYDLAKVG RYKFNKKLAL KARIANHKSA KRIVNPITGE
VLVEEGEKIT REKAEEIQNC GINEVEILVD GKVVKVVGNN TVDINKYPMP YDVSSLNIKE
LVNVRVLREI LDNFSDEEAV LNEIKNRMDE LVPKHITKDD IVATISYQLN LTHGVGFVDD
IDHLGNRRVR SVGELLQNQF RIGLARLERV VKERMTIQDV NEVTPQNLIN IRPVVAAIRE
FFGSSQLSQF MDQTNPLSEL THKRRVSALG PGGLSRERAG FEVRDVHYSH YGRICPIETP
EGPNIGLITS LTTYARVNEY GFIETPYRKV DKATGTVTDE IVYMTADEED EYIIAQANEP
LDENNRFIND RVVCRLREEI ISVPPTEVDF MDVSPKQIVS VSASLIPFLE NDDANRALMG
SNMQRQAVPL LKPEAPIVGT GMEYKAAVDS GAVILAKNDG IVEKVTADKV VIRTKDGKRD
EYHLLKFKRS NQGTCINQRP IVNEGDEVKK GQVICDGPST DHGELALGKN VLVGFMPWEG
YNYEDAILIS EELVMDDSLT SIHIEEYDAE ARDTKLGPEE ITREIPNVGE DALKDLDERG
IIRIGAEVRA GDILVGKVTP KGETELTAEE RLLRAIFGEK AREVRDTSLR VPHGESGIVV
DVKVYSRENG DELPPGVNQM VRVFVAQKRK ISVGDKMAGR HGNKGVISRI LPVEDMPFLP
DGTPLQICLN PLGVPSRMNI GQVLETHLGL VAKALGWYIA SPVFDGATEK DIEELLAKSG
FSPDGKVQLY DGRTGEPFDN KVTVGYMYML KLHHLVDDKM HARSTGPYSL VTQQPLGGKA
QFGGQRFGEM EVWALEAYGA AHTLQEMLTV KSDDVTGRVK AYEAIVKGEN IPEPGIPESF
KVLVKELQSL ALDVKVITED GQEIPIKEFE DDDDDVPDAT LNINIEGRED APPEEVYEEE
YEEEPEELPE DIDFEPDNFD IDSEDLFMDD DYDG
