RPOB_CAUSK
ID RPOB_CAUSK Reviewed; 1360 AA.
AC B0SUP7;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Caul_0794;
OS Caulobacter sp. (strain K31).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC Caulobacteraceae; Caulobacter; unclassified Caulobacter.
OX NCBI_TaxID=366602;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K31;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Thompson L.S., Brettin T.,
RA Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L.,
RA Kyrpides N., Kim E., Stephens C., Richardson P.;
RT "Complete sequence of chromosome of Caulobacter sp. K31.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000927; ABZ69925.1; -; Genomic_DNA.
DR RefSeq; WP_012284864.1; NC_010338.1.
DR AlphaFoldDB; B0SUP7; -.
DR SMR; B0SUP7; -.
DR STRING; 366602.Caul_0794; -.
DR PRIDE; B0SUP7; -.
DR EnsemblBacteria; ABZ69925; ABZ69925; Caul_0794.
DR KEGG; cak:Caul_0794; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1360
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000086364"
SQ SEQUENCE 1360 AA; 151127 MW; 6A5531F6D601A849 CRC64;
MAQSFTGKKR IRKSFGRIPE AVQMPNLIEV QRSSYEQFLQ REVRPGVRKD EGIEAVFKSV
FPIKDFNERA VLEYVSYEFE EPKYDVEECI QRDMTFAAPL KVKLRLIVFE TEEETGARSV
KDIKEQDVYM GDIPLMTDKG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HASGKLLFAA
RVIPYRGSWL DFEFDAKDVV YVRIDRRRKL PATTFLYALG MDGEEILTTF YDVVPFEKRT
AAGAEGWATP YKPERWRGVK PEFALIDADS GEEVAAAGTK ITARQAKKLA DNGLKTLLLA
PEALTGRYLA RDAVNFETGE IYAEAGDELD VTSIEALAAQ GFSTIDVLDI DHVTVGAYMR
NTLRVDKNAV REDALFDIYR VMRPGEPPTV EAAEAMFKSL FFDAERYDLS AVGRVKMNMR
LELDASDEMR VLRKEDVLAV LKLLVGLRDG RGEIDDIDNL GNRRVRSVGE LLENQYRVGL
LRMERAIKER MSSVDIDTVM PHDLINAKPA AASVREFFGS SQLSQFMDQT NPLSEITHKR
RLSALGPGGL TRERAGFEVR DVHPTHYGRI CPIETPEGPN IGLINSLATH ARVNKYGFIE
SPYRRVADGK PLEEVVYMSA MEESKHVIAQ SNIKVLNGEI VDDLVPGRIN GEPTLLQKEQ
VDLMDVSPRQ VVSVAAALIP FLENDDANRA LMGSNMQRQA VPLVQSDAPL VGTGMEAVVA
RDSGAVVIAK RTGVVEQIDG TRIVVRATEE TDAARSGVDI YRLSKFQRSN QSTCINQRPL
VKVGDKISAG DIIADGPSTE LGELALGRNA LVAFMPWNGY NFEDSILISE RIVRDDVFTS
IHIEEFEVMA RDTKLGPEEI TRDIPNVGEE ALRNLDEAGI VAIGAEVQPG DILVGKVTPK
GESPMTPEEK LLRAIFGEKA SDVRDTSLRL PPGVAGTIVD VRVFNRHGVD KDERALAIER
AEIDRLGKDR DDEFAILNRN ISGRLRELLI GNVALSGPKG LSRGQITAEG LAEVQPGLWW
QIALEDEKAM GELESLRRLF DENRKRLDRR FEDKVDKLQR GDELPPGVMK MVKVFVAVKR
KLQPGDKMAG RHGNKGVISR ILPIEDMPFL ADGTHVDVVL NPLGVPSRMN VGQIFETHLG
WACAGLGKQI TTLLEDWQAG GQKQALIDRL TEIYGPDEEL PETEEELVEL ARNLGKGVPI
ATPVFDGARI GDIEDHLRMA GLDPSGQSIL YDGQTGEQFK RPVTVGYIYM LKLHHLVDDK
IHARSIGPYS LVTQQPLGGK AQFGGQRFGE MEVWALEAYG AAYTLQEMLT VKSDDVAGRT
KVYESIVRGD DTFEAGIPES FNVLVKEMRS LGLNVELENS
