ID   RPOB_CAUVC              Reviewed;        1356 AA.
AC   Q9AAU2;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CC_0502;
OS   Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=190650;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19089 / CB15;
RX   PubMed=11259647; DOI=10.1073/pnas.061029298;
RA   Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E.,
RA   Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R.,
RA   Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B.,
RA   DeBoy R.T., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F.,
RA   Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R.,
RA   Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O.,
RA   Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M.;
RT   "Complete genome sequence of Caulobacter crescentus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE005673; AAK22489.1; -; Genomic_DNA.
DR   PIR; E87311; E87311.
DR   RefSeq; NP_419321.1; NC_002696.2.
DR   RefSeq; WP_010918390.1; NC_002696.2.
DR   AlphaFoldDB; Q9AAU2; -.
DR   SMR; Q9AAU2; -.
DR   STRING; 190650.CC_0502; -.
DR   EnsemblBacteria; AAK22489; AAK22489; CC_0502.
DR   KEGG; ccr:CC_0502; -.
DR   PATRIC; fig|190650.5.peg.512; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   BioCyc; CAULO:CC0502-MON; -.
DR   Proteomes; UP000001816; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1356
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047877"
SQ   SEQUENCE   1356 AA;  150890 MW;  BA113BA0EB7F6B51 CRC64;
     MAQSFTGKKR IRKSFGRIPE AVQMPNLIEV QRSSYEQFLQ RETRPGLRRD EGVEAVFKSV
     FPIKDFNERA VLEYVSYEFE EPKYDVEECI QRDMTFAAPL KVKLRLIVFE TEEETGARSV
     KDIKEQDVYM GDIPLMTDKG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HASGKLLFAA
     RVIPYRGSWL DFEFDAKDIV YVRIDRRRKL PATTFLYALG MDGEEILTTF YDVVPFEKRS
     GGWATPYKPE RWRGVKPEFP LVDADTGEEV APAGTKITAR QAKKFADGGL KTLLLAPEAL
     TGRYLARDAV NMATGEIYAE AGDELDVTSI QALADQGFST IDVLDIDHVT VGAYMRNTLR
     VDKNAIREDA LFDIYRVMRP GEPPTVEAAE AMFKSLFFDA ERYDLSSVGR VKMNMRLEQD
     VSDEVRILRK EDVLAVLKVL VGLRDGRGEI DDIDNLGNRR VRSVGELLEN QYRVGLLRME
     RAIKERMSSV DIDTVMPHDL INAKPAAAAV REFFGSSQLS QFMDQTNPLS EITHKRRLSA
     LGPGGLTRER AGFEVRDVHP THYGRICPIE TPEGPNIGLI NSLATHARVN KYGFIESPYR
     RVKDGKPQDE VVYMSAMEES KHVIAQSNIK VAEGEIVEDL VPGRINGEPT LLQKETVDLM
     DVSPRQVVSV AAALIPFLEN DDANRALMGS NMQRQAVPLV QSDAPLVGTG MEAVVARDSG
     AVVIAKRTGV VEQIDGTRIV IRATEETDPA RSGVDIYRMS KFQRSNQSTC INQRPLVKVG
     DRIVAGDIIA DGPSTELGEL ALGRNALVAF MPWNGYNFED SILISERIVR DDVFTSIHIE
     EFEVMARDTK LGPEEITRDI PNVGEEALRN LDEAGIVAIG AEVQPGDILV GKVTPKGESP
     MTPEEKLLRA IFGEKASDVR DTSLRLPPGV AGTIVDVRVF NRHGVDKDER ALAIERAEID
     RLGKDRDDEF AILNRNISGR LKELLIGKVA LSGPKGLSRG EITAEGLAQV ASGLWWQIAL
     EDEKAMGELE SLRRLFDENR KRLDRRFEDK VDKLQRGDEL PPGVMKMVKV FVAVKRKLQP
     GDKMAGRHGN KGVISRILPI EDMPFLADGT HVDVVLNPLG VPSRMNVGQI FETHLGWACA
     NLGKQITNLL EDWQQGGQKQ ALVERLTEIY GPDEELPDTE EGLVELARNL GKGVPIATPV
     FDGARMDDIE GHLEMAGVNK SGQSILFDGL TGEQFKRPVT VGYIYMLKLH HLVDDKIHAR
     SIGPYSLVTQ QPLGGKAQFG GQRFGEMEVW ALEAYGAAYT LQEMLTVKSD DVAGRTKVYE
     SIVRGDDTFE AGIPESFNVL VKEMRSLGLN VELENS