ID   RPOB_CERDE              Reviewed;        1070 AA.
AC   A8SE86;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Ceratophyllum demersum (Rigid hornwort) (Coontail).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ceratophyllales; Ceratophyllaceae;
OC   Ceratophyllum.
OX   NCBI_TaxID=4428;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=18048334; DOI=10.1073/pnas.0708072104;
RA   Moore M.J., Bell C.D., Soltis P.S., Soltis D.E.;
RT   "Using plastid genome-scale data to resolve enigmatic relationships among
RT   basal angiosperms.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:19363-19368(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; EF614270; ABQ81442.1; -; Genomic_DNA.
DR   RefSeq; YP_001542439.1; NC_009962.1.
DR   AlphaFoldDB; A8SE86; -.
DR   SMR; A8SE86; -.
DR   GeneID; 5729428; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000329198"
SQ   SEQUENCE   1070 AA;  120554 MW;  80AB8769A647239E CRC64;
     MLRDRNEGMS TIPGFSQIQF EGFCRFLDQG LTEELHKFPK IEDTDQEIEF QLFVETYQLA
     EPLIKEKDAI YESLTYSSEL YVPAGLIWKN GRDMQEQTVF IGNIPLMNSL GTSIVNGIYR
     IVINQILQSP GIYYRSELDH NGISVYTGTI ISDWGGRSEL EIDRKARIWA RVSRKQKISI
     LVLSSAMGSN LREILDNVCY PEIFLSFLND KDRKKIGSKE NAILEFYQQF ACVGGDPVFS
     ESLCKELQKK FFQQRCELGR IGRRNMNQRL NLNIPQNNTF LLPRDVLAAA DHLIGMKFGM
     GTLDDMNHLK NKRIRSVADL LQDQFGLALV RLENAIRGTI SGAIKHKLIP TPQNLVTSTP
     LTTTFESFFG LHPLSQVLDR TNPLTQIVHG RKSSYLGPGG VTGRTASFRI RDIHPSHYGR
     ICPIDTSEGI NVGLIGSLAI HAKIGFWGSI ETPFYEISEK SKEAQIVYLP PSRDEYSMVA
     AGNSLALNRG IQEEQVVPAR YRQEFLTIAW EKIHLRSIFP FQYFSIGASL IPFIEHNDAN
     RALMSSNMQR QAVPLFRSEK CIVGTGLERQ VALDSGVSAI AEHEGKILYT DTDKIVLSAN
     GDTISIPLVM YQRSNKNTCM HQKPQVQRNK CIKKGQILAD GAATVGGELA LGKNVLVAYM
     PWEGYNSEDA VLISERMVYE DIFTSFHIRK YEIQTHVTSQ GPERITNEIP HLEAHLLRNL
     DRNGIVRLGS WIETGDILVG KLTPQTAKES SYAPEDRLLR AILGVQVSTA KETCLKLPIG
     GRGRVIDVRW IQKKGGSSYN PETIRVYISQ KREIKVGDKV AGRHGNKGII SKILARQDMP
     YLQDGTPIDM VFNPLGVPSR MNVGQIFECS LGLAGDLLDR HYRIAPFDER YEQEASRKLV
     FPELYEASKQ TSNPWVFEPE YPGKSRIFDG RTGDPFEQPV IIGKSYILKL IHQVDDKIHG
     RSSGHYALVT QQPLRGRAKQ GGQRVGEMEV WALEGFGVAH ISQEMLTYKS DHIRARQEVL
     GTTIIGGTIP NPEDAPESFR LLVRELRSLA LELNHFLISD KNFQINRKEA