ID   RPOB_CERS1              Reviewed;        1377 AA.
AC   A3PGI9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB1 {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=Rsph17029_0339;
GN   and
GN   Name=rpoB2 {ECO:0000255|HAMAP-Rule:MF_01321};
GN   OrderedLocusNames=Rsph17029_0353;
OS   Cereibacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) (Rhodobacter
OS   sphaeroides).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Cereibacter.
OX   NCBI_TaxID=349101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17029 / ATH 2.4.9;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.,
RA   Mackenzie C., Choudhary M., Donohue T.J., Kaplan S.;
RT   "Complete sequence of chromosome 1 of Rhodobacter sphaeroides ATCC 17029.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP000577; ABN75455.1; -; Genomic_DNA.
DR   EMBL; CP000577; ABN75469.1; -; Genomic_DNA.
DR   RefSeq; WP_002722478.1; NC_009049.1.
DR   AlphaFoldDB; A3PGI9; -.
DR   SMR; A3PGI9; -.
DR   EnsemblBacteria; ABN75455; ABN75455; Rsph17029_0339.
DR   EnsemblBacteria; ABN75469; ABN75469; Rsph17029_0353.
DR   GeneID; 57469046; -.
DR   KEGG; rsh:Rsph17029_0339; -.
DR   KEGG; rsh:Rsph17029_0353; -.
DR   HOGENOM; CLU_000524_4_0_5; -.
DR   OMA; FMTWEGY; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1377
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000300384"
SQ   SEQUENCE   1377 AA;  153818 MW;  43FBB838B235C6F5 CRC64;
     MAQSYVGQKR IRRYYGKIRE VLEMPNLIEV QKSSYDLFLK SGDGPKAADG EGIQGVFQSV
     FPIKDFNETA VLEFVKYELE KPKYDVDECQ QRDMTYAAPL KVTLRLIVFD VDETTGARSV
     KDIKEQDVYM GDMPLMTANG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAC
     RIIPYRGSWL DFEFDAKDIV FARIDRRRKL PVTTLLYALG MDQEGIMDAY YETVNFKHQK
     NRGWVTRFFP ERVRGTRPTY DLVDAATGEV ILKAGEKATP RMVKKWIDEA QITELLVPFD
     HIVGRYVAQD IINEETGEIW VEAGDELTME YDRDGEVKGG TLKLLLDQGI TDIPVLDIDN
     VNVGPYIRNT MAADKNMGRD TALMDIYRVM RPGEPPTVEA ASNLFDTLFF DSERYDLSAV
     GRVKMNMRLD LGKPDTQRTL DRDDIIACIK ALTELRDGKG EIDDIDHLGN RRVRSVGELM
     ENQYRVGLLR MERAIKERMS SVEIDTIMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP
     LSEVTHKRRL SALGPGGLTR ERAGFEVRDV HPTHYGRMCP IETPEGQNIG LINSLATFAR
     VNKYGFIETP YRKVVEGAVT DDVVYMSATE EMRHTVAQAN AQLDEEGRFV SDLISSRKAG
     EFMLNPPDAI DLIDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLLQSDAPFV
     GTGIEAVVAR DSGAAIMARR AGVIDQVDAT RIVVRATEML EPGEPGVDIY RLRKFKRSNQ
     SSCINQRPLV KVGDVVHRGE VVADGPCTDM GELALGRNVI VAFMPWNGYN YEDSILISER
     ILRDDVYTSI HIEEYEVAAR DTKLGPEEIT RDIPNVGEEA LRNLDEAGIV YIGAEVQPGD
     ILVGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSLRLP PGAYGTIVEV RVFNRHGVDK
     DERALQIERE EVERLARDRD DELAILERNI YSRLRTLIMG KTAVKGPKGI RAGSEINEDL
     LSTLSRGQWW QLALGEEADA KEVEALHEQF EAQKRALDHR FEDKVEKVRR GDDLPPGVMK
     MVKVFVAVKR KLQPGDKMAG RHGNKGVISK VVPIEDMPFL ADGTHVDLVL NPLGVPSRMN
     VGQILETHMG WAARGLGIKI DEALQDYRRS GDLTPVKEAM RLAYGDETYE GAFGDREDED
     LVEMAGRVTK GVPIATPVFD GAKEPDVNDA LRRAGFDQSG QSIVFDGRTG EQFARPVTVG
     VKYMLKLHHL VDDKLHARST GPYSLVTQQP LGGKAQFGGQ RLGEMEVWAL EAYGAAYTLQ
     EMLTVKSDDV AGRTKMYESI VKGEDNFEAG VPESFNVLVK EVRGLGLNME LLDADEE