RPOB_CERS5
ID RPOB_CERS5 Reviewed; 1377 AA.
AC A4WVL6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
GN OrderedLocusNames=Rsph17025_2542;
OS Cereibacter sphaeroides (strain ATCC 17025 / ATH 2.4.3) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=349102;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17025 / ATH 2.4.3;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Richardson P., Mackenzie C., Choudhary M.,
RA Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome of Rhodobacter sphaeroides ATCC 17025.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC and 1 omega subunit. When a sigma factor is associated with the core
CC the holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR EMBL; CP000661; ABP71430.1; -; Genomic_DNA.
DR AlphaFoldDB; A4WVL6; -.
DR SMR; A4WVL6; -.
DR STRING; 349102.Rsph17025_2542; -.
DR PRIDE; A4WVL6; -.
DR EnsemblBacteria; ABP71430; ABP71430; Rsph17025_2542.
DR KEGG; rsq:Rsph17025_2542; -.
DR eggNOG; COG0085; Bacteria.
DR HOGENOM; CLU_000524_4_0_5; -.
DR OMA; FMTWEGY; -.
DR OrthoDB; 9601at2; -.
DR BioCyc; RSPH349102:G1G8M-2620-MON; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR02013; rpoB; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW Transferase.
FT CHAIN 1..1377
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_1000051976"
SQ SEQUENCE 1377 AA; 153802 MW; FBD14C2E2A426FE3 CRC64;
MAQSYVGQKR IRRYYGKIRE VLEMPNLIEV QKSSYDLFLK SGEGPKAADG EGIQGVFQSV
FPIKDFNETA VLEFVKYELE KPKYDVDECQ QRDMTYAAPL KVTLRLIVFD VDETTGARSV
KDIKEQDVYM GDMPLMTANG TFIVNGTERV IVSQMHRSPG VFFDHDKGKT HSSGKLLFAC
RIIPYRGSWL DFEFDAKDIV FARIDRRRKL PVTTLLYALG MDQEGIMDAY YDTVDFKYQK
NRGWVTRFFP ERVRGTRPTY DLVDAATGEV ILKAGEKATP RMVKKWIDDA QITELLVPFD
HIVGRYVARD IINEETGEIW VEAGDELTME YDRDGEVKGG TLKLLLDQGI TDIPVLDIDN
VNVGPYIRNT MAADKNMGRD TALMDIYRVM RPGEPPTVEA AAALFDTLFF DSERYDLSAV
GRVKMNMRLD LGKPDTQRTL DRDDIIACIK ALTELRDGKG EIDDIDHLGN RRVRSVGELM
ENQYRVGLLR MERAIKERMS SVEIDTIMPQ DLINAKPAAA AVREFFGSSQ LSQFMDQTNP
LSEVTHKRRL SALGPGGLTR ERAGFEVRDV HPTHYGRMCP IETPEGQNIG LINSLATFAR
VNKYGFIETP YRKVIEGQVT DDVVYMSATE EMRHTVAQAN ATLDEEGRFV SDLISSRKAG
EFMLNPPDAI DLIDVSPKQL VSVAASLIPF LENDDANRAL MGSNMQRQAV PLLQSDAPFV
GTGIEAVVAR DSGAAIMARR AGVIDQVDAT RIVVRATEML EPGEPGVDIY RLRKFKRSNQ
SSCINQRPLV KVGDVVHRGE VVADGPCTDM GELALGRNVV VAFMPWNGYN YEDSILISER
ILRDDVYTSI HIEEYEVAAR DTKLGPEEIT RDIPNVGEEA LRNLDEAGIV YIGAEVQPGD
ILVGKITPKG ESPMTPEEKL LRAIFGEKAS DVRDTSLRLP PGAYGTIVEV RVFNRHGVDK
DERALQIERE EVERLARDRD DELAILERNI YSRLKTLIMG KVAVKGPKGI KAGSEINDEL
LSTLSRGQWW QLALGEEADA KEVEALHEQF EAQKRALDHR FEDKVEKVRR GDDLPPGVMK
MVKVFVAVKR KLQPGDKMAG RHGNKGVISK VVPIEDMPFL ADGTHVDLVL NPLGVPSRMN
VGQILETHMG WAARGLGIQI DEALQDYRRS GDLTPVKEAM RLAYGDETYE GAFADRDDED
LVEMASRVTK GVPIATPVFD GAKEPDVNDA LRRAGFDQSG QSIVFDGRTG EQFARPVTVG
VKYMLKLHHL VDDKLHARST GPYSLVTQQP LGGKAQFGGQ RLGEMEVWAL EAYGAAYTLQ
EMLTVKSDDV AGRTKMYESI VKGEDNFEAG VPESFNVLVK EVRGLGLNME LLDADEE
