ID   RPOB_CHAGL              Reviewed;        1070 AA.
AC   Q8MA12;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Chaetosphaeridium globosum (Charophycean green alga) (Herposteiron
OS   globosum).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Coleochaetophyceae; Coleochaetales;
OC   Chaetosphaeridiaceae; Chaetosphaeridium.
OX   NCBI_TaxID=96477;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M1311;
RX   PubMed=12161560; DOI=10.1073/pnas.162203299;
RA   Turmel M., Otis C., Lemieux C.;
RT   "The chloroplast and mitochondrial genome sequences of the charophyte
RT   Chaetosphaeridium globosum: insights into the timing of the events that
RT   restructured organelle DNAs within the green algal lineage that led to land
RT   plants.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:11275-11280(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AF494278; AAM96566.1; -; Genomic_DNA.
DR   RefSeq; NP_683774.1; NC_004115.1.
DR   AlphaFoldDB; Q8MA12; -.
DR   SMR; Q8MA12; -.
DR   PRIDE; Q8MA12; -.
DR   GeneID; 860722; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 3.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1070
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048015"
SQ   SEQUENCE   1070 AA;  121062 MW;  37B1F2D77619314B CRC64;
     MTLQTNQTEK FTLPDLGKIQ LQNFYNFLTK IIYKELKAFP KIYDSEHNFE FKIYPEEFFL
     VEPILNERES IYKSITYSTE LYVTAELNCI KNQTKQKQKV LLGHIPLITS NGSFIINGIS
     RAVVSQILRS PGIYFGSQLD SNSKCIYTAT IISESGSRLK LEIDDRKLIW ARISKKRKVS
     AIVLLLSMGI KLSDITRSIE HPAILDNILK KKNVLSNQKD AILEIYKQLF SAGGDPNFSD
     NILSELQRTF FQQRCEIGLI GRSNLNKKLG LNIPDEEIFL LPEDILATIN YLIKLSLGIG
     SLDDIDHLKN RRVKSISDFL SEQLKNALNK IENTIEQSIK NISKRKRILT PKSLINSGIL
     LAFFKDFFGS HPLSQFLDQT NSLTELVHKR RISSLGPGGL TRRTASFHVR DIHPSHYGRI
     CPIETSEGMN AGLVASLASN ATINTMGFIE SPFYQLNDSS LFDKPIFLSA REDENFRIAL
     GNRLAIDQEI QEKQITPARY KQEFINTPWS LANLRSIFPW QYFSIGASLI PFLEHDDANR
     ALMGSNMQRQ AVPLLITERP IVGTGFEANI ARDSGAPLIS NIDGKIQYLD SEFIEILGVD
     KKTYKLKLSN YQRSNNNTCI HHRAIVQLNQ WVRKGQILAD GATTIRGELS LGRNILVAYM
     PWEGYNFEDA VLISDRLIYD DLYTSIHIQK YELEIKINHQ GQDKITKDIP HIDKYFLRHL
     DENGLVTLGS WVEAGDVLIG KLSSQEREDS LKVPEGKLLQ AIFGLQIGNS RDTSLKLPAG
     GEGRVIDIQW IFQDEGGIDS NNFVNVYVLQ KRRIQVGDKI AGRHGNKGIV SQILSRHDMP
     YLQNGTSIDM VLSPLGVPSR MNVGQILECL LGFAGKFLNK NYRITPFDER YEREASRKLV
     LSELHKAKKL TGYPWLLEPE NAGKSRLFDG RTGETFNQPV TVGSAYILKL IHQVDDKIHA
     RSTGPYSLVT QQPVRGRSRG GGQRVGEMEV WALEGFGAAY ILQEMLTVKS DHIHSRYDVL
     GAIVTGTTIK TPTTTPESFR LLARELRCLG IQINDIEIYH ETLQKKEIEI