RPOB_CHAVU
ID RPOB_CHAVU Reviewed; 1074 AA.
AC Q1ACN6;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS Chara vulgaris (Common stonewort).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae;
OC Chara.
OX NCBI_TaxID=55564;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16611644; DOI=10.1093/molbev/msk018;
RA Turmel M., Otis C., Lemieux C.;
RT "The chloroplast genome sequence of Chara vulgaris sheds new light into the
RT closest green algal relatives of land plants.";
RL Mol. Biol. Evol. 23:1324-1338(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000255|HAMAP-Rule:MF_01321}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABA61980.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; DQ229107; ABA61980.1; ALT_INIT; Genomic_DNA.
DR RefSeq; YP_635711.1; NC_008097.1.
DR AlphaFoldDB; Q1ACN6; -.
DR SMR; Q1ACN6; -.
DR GeneID; 4100250; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.30.150.10; -; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR010243; RNA_pol_bsu_bac.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1074
FT /note="DNA-directed RNA polymerase subunit beta"
FT /id="PRO_0000276583"
SQ SEQUENCE 1074 AA; 122226 MW; 60728D2CB5C891A5 CRC64;
MKFEKNTDIF ILPDFIRIQL ESFHRFIEKG LLEEFQRFPV IRDSSGELSY FLNGKRYKLQ
EPSFNEQEAI YQASTYSSEL YVPAQITHTK SEKTKSEIIL IGNIPIMTAR GNFIINGASR
VIVNQILRSP GLYYGSELDA QANITYSCTF ISEWGGRLKL EMDNKRRIWV RLSKKRKIPV
LLLLLAMGLN WKDLEKLISR PSLLLSYLSK EKRTPYSPEE AIIELYRHSC SVSGEISFSE
NIRKELYHKF FKIRCEIGSI GRLNINKKLN LDIPEEERFL VPQDLIAAIN SLIEKSFKIV
NLDDIDHLKN RRVHLIADML QSQVRIALNR LKRMIAEVMR GINKRKNLPN PQSLVTPKPL
VATFQEFFGS HPLCQFMDQT NPLAEITHKR RISSLGPGGL TQKTATFNVR DIHPSHYGKI
CPIETPEGQN AGLVSSLAIL ANINQNGFIE SPFLKLHKNE YKQLTGSEHL CSEQEEYYRI
TTGEHSRETA EKKGKPTPIR YRQEFSATAF HFLHFRDILP IQYFSIATSL IPFLEHDDAN
RALMGSNMQR QAVPLLRPEK PIVGTGLEGQ VALDSAALVL SKIEGKVDFV DGRNINIRSN
IVINEFFSFQ LINYERSNQN TCLHQKPIVK KDDYVKKGEL LADASSTLGG EISLGNNILV
AYMPWEGYNF EDAVLINERL IYEDIYTSIH IEKYEIEVNM TSLGPEKITK KIPHLSEYLL
RNLDVNGLIL LGSWVKTGDV LVGKLTPREP GESLRLPEGR LLHAIFGIKA SSFFETCLKV
APGAEGRVID VRWVESANKL TQKYIKTVNI YILQKRKIQV GDKIAGRHGN KGVISRILPR
EDMPYIQDGT PIDMVLSPLG VPSRMNLGQI FECLLGLAGE SLKKHYRIMP FDERHEREAS
RKLAFSELYQ AKKFTGHSWL FEPDTPGKSQ LFDGRTGQTF ENAITVGKAY ILKLIHQVDD
KIHARSTGPY SMVTQQPLGG KARRGGQRMG EMEVWALEGF GAAYTLQELL TRKSDDMKGR
NEALGAIVTG NLIPKPNTTP ESFKLLMREL RCLCLNIHHC SLFQENLSMK NIEF
