ID   RPOB_CHLSY              Reviewed;        1227 AA.
AC   B9LL91;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=Chy400_0949;
OS   Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl).
OC   Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC   Chloroflexaceae; Chloroflexus.
OX   NCBI_TaxID=480224;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29364 / DSM 637 / Y-400-fl;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Kiss H., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ovchinnikova G., Bryant D.A., Richardson P.;
RT   "Complete sequence of Chloroflexus sp. Y-400-fl.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; CP001364; ACM52372.1; -; Genomic_DNA.
DR   RefSeq; WP_012256763.1; NC_012032.1.
DR   AlphaFoldDB; B9LL91; -.
DR   SMR; B9LL91; -.
DR   KEGG; chl:Chy400_0949; -.
DR   HOGENOM; CLU_000524_4_1_0; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 1.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Transcription;
KW   Transferase.
FT   CHAIN           1..1227
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_1000165799"
SQ   SEQUENCE   1227 AA;  137772 MW;  48929BEF18908EE5 CRC64;
     MPPLIESVVL QPLIAPIDPG ADGRRSRRIE RRSFARIKDA IDLPLLIETQ LKSFEWFKRE
     GLRELFDEIS PITDFTGKNL ELHFRDYTFG EPRYDEFECR ERDLTYAAPL RVRVELRILT
     TGEIKESEIF LGDFPIMTDN GTFVYNGAER VVVSQLIRSP GVYFKDEKEP TSGRSLHTAK
     LIPNRGAWLE FETNKRDVIS VKVDRKRKIP VTILLRAITA WIAEENGNGR WVPDNELDKF
     GHNDQIIELF RHVDTVPEHP YIHATLDKDP SRNAKEALLE LYKRLRPGDP PTLENARSLI
     ESLLFSPRRY DLAKVGRYKL NKNLWERDVR RDGAKAPDLS VRVLLPRDIF RIVEQLILLN
     NGHGRPDDID HLGNRRVRTV GELIQQQFRV GLLRLERVVK ERMSLQDPAS ATPNGLINIR
     PVVAAMREFF GGSQLSQFMD QTNPLAELTN KRRLSALGPG GLSRDRAGFE VRDVHHSHYG
     RICPVETPEG PNIGLIGTMS TFARVNEMGF LETPYRKVYN SVDNVQVWKE KGILLRDVRD
     LRTGDLIAAK GTRVNDEIAR QITIGLLRGQ ILREDIVDPD TDELIAEAGT EINRALAERI
     VELPIKHIKI RPVVSQEVDY LSADEEDRFV IVQANAPLDQ HNRFLDTIVS CRFGEDFVSE
     RVERVDYMDV SPKQVVSVST SLIPFLEHDD ANRALMGSNM QRQAVPLLRP DAPIVGTGME
     YRAARDSGQV IVARRDGVVV STTSERIVIE EDDGNQTEYR LRKFMRSNQD TCINQRPAVV
     RGQRVKAGDV IADSSSTDQG ELALGQNVLV AYMPWEGGNF EDAILVSERL VREDIFTSIH
     IEKYEVEARD TKLGPEEITR DIPNVGQESL RNLDERGIIY IGAEVQPNDI LVGKITPKGE
     TDLTAEERLL RAIFGEKARE VKDSSLRVPN GVRGKVIDVK VFSRSEGAEL PVGVNQTVRV
     LLCQKRKISA GDKMAGRHGN KGVVSRVLPI EDMPFLPDGR PVDIILNPIG VPSRMNIGQI
     LETHLGWAAA RLGFRVATPV FDGAHEDQIK DLLVQAGLPA DGKVTLYDGR TGERFDNPVT
     VGYAYMLKLA HLVEDKIHAR STGPYSLVTQ QPLGGKAQFG GQRFGEMEVW ALEAYGAAYT
     LQEMLTVKSD DVVGRVKTYE AIVKGEPIQE AGVPESFKVL IKELQSLGLS VEVLSADEKP
     VELSDDLDSD IGALEGINLS GMERGEF