ID   RPOB_CHLTE              Reviewed;        1303 AA.
AC   Q8KG15;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNAP subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Transcriptase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321}; OrderedLocusNames=CT0155;
OS   Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
OS   (Chlorobium tepidum).
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobaculum.
OX   NCBI_TaxID=194439;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49652 / DSM 12025 / NBRC 103806 / TLS;
RX   PubMed=12093901; DOI=10.1073/pnas.132181499;
RA   Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J.,
RA   DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D.,
RA   Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M.,
RA   Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V.,
RA   Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M.,
RA   White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A.,
RA   Fraser C.M.;
RT   "The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic,
RT   anaerobic, green-sulfur bacterium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta'
CC       and 1 omega subunit. When a sigma factor is associated with the core
CC       the holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AE006470; AAM71403.1; -; Genomic_DNA.
DR   RefSeq; NP_661061.1; NC_002932.3.
DR   AlphaFoldDB; Q8KG15; -.
DR   SMR; Q8KG15; -.
DR   STRING; 194439.CT0155; -.
DR   EnsemblBacteria; AAM71403; AAM71403; CT0155.
DR   KEGG; cte:CT0155; -.
DR   PATRIC; fig|194439.7.peg.152; -.
DR   eggNOG; COG0085; Bacteria.
DR   HOGENOM; CLU_000524_4_0_10; -.
DR   OMA; FMTWEGY; -.
DR   OrthoDB; 9601at2; -.
DR   Proteomes; UP000001007; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 2.40.50.150; -; 1.
DR   Gene3D; 3.90.1110.10; -; 2.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 3.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   TIGRFAMs; TIGR02013; rpoB; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           1..1303
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000047881"
SQ   SEQUENCE   1303 AA;  145469 MW;  365DF70F97A2042C CRC64;
     MKVADATPTP CIDFSKIQSI INPPDLLKVQ LDSFHNFIQD SVPLEKRKDQ GLEKVLRSAF
     PITDTRGLYL LEYISYSFDK PKYTVEECIE RGLTYDVSLK IKLKLSYKDE ADEPDWKETI
     QQEVYLGRIP YMTDRGTFII NGAERVVVAQ LHRSPGVVFS EAVHPNGKKM YSAKIVPTRG
     SWIEFQTDIN NQIFVYIDQK KNFLVTALLR AIGFAKDEDI LGLFDLVEEV EVSSKSSKRE
     QLLGQYLASD IIDMTTGEVV PARAAITEEI IDQIVAAGYK TVKVMKTTSP EKGVDKSVII
     NTILNDSSAT EEEALEIVYE ELRSNEAPDI DAARSFLERT FFNQKKYDLG DVGRYRIQKK
     LQNELAELSA YLEKRPELKE LSDAIYERIL QTISTYSEEP IGEDILVLTH YDIIAVINYL
     IKLINGMAEV DDVDHLANRR VRSVGEQLAA QFVIGLARMG KNVREKLNSR DTDKIAPADL
     INARTVSSVV SSFFATSQLS QFMDQTNPLA EMTNKRRVSA LGPGGLTRER AGFEVRDVHY
     THYGRLCPIE TPEGPNIGLI SSLSVYAEIN DKGFIQTPYR VVENGQVTDK VVMLSAEDEE
     NKITVPVSIE LDENNRIAAE SVQARTKGDY PLVLAEEVNY MDVSPVQIVS AAAALIPFLE
     HDDGNRALMG ANMQRQAVPL LVSEAPIVGT GMEAKVARDS RAVIVAEGPG VVQCVTADRI
     EVRYDLDPEN NTVSLLDPDE GVKVYKLIKF KRSNQDTCIS QRPLVHNGQR VNAGDVLADS
     SSTDNGELAL GKNVLVAFMP WRGYNFEDAI VLSERLVYDD VFTSIHVHEF ESSVRDTKRG
     EEQFTRDIYN VSEDALRNLD ENGIVRIGAE VKERDILVGK ITPKGESDPT PEEKLLRAIF
     GDKSSDVKDA SMHVPAGMKG IVIKTKLFSR KKKIGMDVKE RMEAIDKQFD LKEADLRSRF
     AKWMKQYLNG KKSVAITSDK GKVLVPEGTV IDEALLAKFN GLPFLESIDL SKGIVSGAKT
     NENVVRLIRE YRLKLKDLSD ERENEKYKIN VGDELPPGIE ELAKVYIAQK RKIQVGDKMA
     GRHGNKGVVG KILPIEDMPF MEDGTPVDIV LNPLGVPSRM NIGQLYETSL GWAGKKLGVK
     FKTPIFSGAT YTEVQEYLEK AGLPGHGKVK LFDGRTGEQF HDEVTVGYIY MLKLSHLVDD
     KIHARSIGPY SLITQQPLGG KAQFGGQRFG EMEVWALEAY GAANILREML TVKSDDVIGR
     NKTYEAIVKG QNLPEPGTPE SFNVLIRELQ GLGLEIRIDD RVP