ID   RPOB_CHLVU              Reviewed;        1301 AA.
AC   P56299;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01321};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
DE            Short=RNA polymerase subunit beta {ECO:0000255|HAMAP-Rule:MF_01321};
GN   Name=rpoB {ECO:0000255|HAMAP-Rule:MF_01321};
OS   Chlorella vulgaris (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC   Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX   NCBI_TaxID=3077;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAM C-27 / Tamiya;
RX   PubMed=9159184; DOI=10.1073/pnas.94.11.5967;
RA   Wakasugi T., Nagai T., Kapoor M., Sugita M., Ito M., Ito S., Tsudzuki J.,
RA   Nakashima K., Tsudzuki T., Suzuki Y., Hamada A., Ohta T., Inamura A.,
RA   Yoshinaga K., Sugiura M.;
RT   "Complete nucleotide sequence of the chloroplast genome from the green alga
RT   Chlorella vulgaris: the existence of genes possibly involved in chloroplast
RT   division.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:5967-5972(1997).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01321};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000255|HAMAP-Rule:MF_01321}.
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DR   EMBL; AB001684; BAA57969.1; -; Genomic_DNA.
DR   PIR; T07321; T07321.
DR   RefSeq; NP_045893.1; NC_001865.1.
DR   AlphaFoldDB; P56299; -.
DR   SMR; P56299; -.
DR   PRIDE; P56299; -.
DR   GeneID; 809111; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   CDD; cd00653; RNA_pol_B_RPB2; 1.
DR   Gene3D; 2.40.270.10; -; 2.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   HAMAP; MF_01321; RNApol_bact_RpoB; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR   InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR   InterPro; IPR010243; RNA_pol_bsu_bac.
DR   InterPro; IPR007121; RNA_pol_bsu_CS.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   InterPro; IPR007641; RNA_pol_Rpb2_7.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR   Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR   PROSITE; PS01166; RNA_POL_BETA; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1301
FT                   /note="DNA-directed RNA polymerase subunit beta"
FT                   /id="PRO_0000048018"
SQ   SEQUENCE   1301 AA;  148422 MW;  EC0AA9401815E178 CRC64;
     MSYKKTLISK NFFFIKALNC SVSAGYVEKT GCKLPVQFSM EIPNLIDVQR QSFYSFIEYG
     LKEAFQKPLR FSTTKHDLEI RFFPEGIQFR KPDFTQKQAV VLGKIYGSAV YIPVGIHSKK
     SSTFRIEWLF VGILPIMTRQ GHFVVNGVSR VVLHQMVRNP GIYTLPIHPR TKIGTLRIVP
     EKGGWIHITV DKKHRVWFLT RSLRRKVSLL IFLQAVGIPF HDIFLRLEHS KILQNSFVAE
     LGEGSARHDD VLERAGLYAH PATQEEAWQY LYSHFKEYSP YAHKNLAAKE QTAREFFYST
     LWNKDKLILG HIGRQQFREK IGSIEPLENT SLTREDLLEA TQALLSLMHK KRVVDEIDSL
     TQKRIRGCDE FLLEHLATGM KEFELFFRRK VTFLPATKSI TGAENPFRSL WRQNKAVFSK
     TVSKSWKRFF TSGTLGQFMD QTNSLAETTH KRRLTVLGPG GISGKQTTIQ IRGIHPTYYG
     RLCPIETPEG KNAGLVNSFT VLSVLSKYGA RTLTTPFYQV YKGQVQKTLP PLRVSPRQEY
     KLIEAPADIQ LTAWNVLPKT HLPVRKELNF HSDVATRITT QSVGILQNIS VATSLIPFLE
     HNDANRALMG SNMQRQAVPL LKPQAPLVGT GLESRVIGDV NHSMQASKTG FITKVSSTKI
     QVLSPRKTKA QVSVFSHVLF SLNKKKKKSL LNSEKQSFSK NGIFFIKTLQ QKSNLKNIFF
     SAQKALYQEN SSFDLKFEAQ NRLFIPKMTF SLAQNFSRKI PFLSFFLKKK KERPRNPKKF
     FRSSDSFVNW KHKKLHSEDF RKNKVEITTT YSLIQYQRTN QSTCFSERPI LPENEWVEKG
     DLLADGASSS QGKLSIGQNV LVAYLPWEGY NFEDAILISQ RLVDQEIFTS LHMDHYDIAV
     QNTQYGLERI TNLIPLKISD STRDIYKMGN LDSRGLVEIG SWVEPGDYLV GKMSPLDPKS
     PPLQSPHEKL YNVILQREKT SFRNTSLRVP KGVQGFVLGV QILPSQEPDV AALAEKDEIL
     RVRVLLLQRR KIQVGDKMAG RHGNKGIVSL ILPRQDMPYL PDGTPVDIVL NPLGVPSRMN
     VGQILECLLG LAGHFLHERY TTYLFDEQYG AEASRSLVYS KLYQASLKTR NPWVFEPHHP
     GKIRVFDGRT GLTFDQPITA GYAYILKLVH LVDDKIHARP TGPYSAITQQ PVKGRARNGG
     QRLGEMEVWA LQAYGAAHTL HEFFTVKSDD LDGRQQAVLN IYANKPVTTG NPESFKLILR
     ELQALCFNFQ VYEKYSYYSE YTQKTRVSIF PISFIKLEDV L